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Different release of cytokines into the cerebrospinal fluid following surgery for intra- and extra-axial brain tumours

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Summary

To elucidate the role of cytokines in brain repair processes and in local inflammation after neurosurgical procedures, cerebrospinal fluid (CSF) samples from 8 patients with intra-axial tumours and 8 patients with extra-axial tumours were analysed for interleukin (IL)-1beta, IL-1 receptor antagonist (IL-1ra), IL-6, IL-8, IL-10, and tumour necrosis factor (TNF)-alpha at the beginning and after surgery. Levels of IL-6 and IL-8 increased dramatically in all patients just hours after surgery and fell during subsequent days. IL-1beta was found only in low amounts in the CSF of both patient groups. Other cytokines demonstrated different courses. In patients with intra-axial tumours IL-1ra peaked two to four hours after surgery with a subsequent decrease. In patients with extra-axial tumours there was a continuous low-level IL-1ra release into the CSF without a peak. TNF-alpha was not present in detectable levels in the CSF after surgery for extra-axial tumours but was found to peak two to four hours after surgery for intra-axial tumours. IL-10 was detected in the CSF of both patient groups, but a higher peak was seen after surgery for extra-axial tumours. These results suggest different requirements for the cytokine response and an involvement of different cell types in cytokine release. However, the analysis of the CSF from both patient groups showed no differences in cell counts and populations, with a mild pleocytosis being present in both patient groups after surgery. Therefore, we conclude that after surgery for extra-axial tumours cytokines were predominately produced by non-immune cells stimulated through hypoxia or mechanical irritation. After surgery for intra-axial tumours with a significant brain injury immune cells — activated by necrotic material —seem to be involved in the process of cytokine synthesis. In these cases an additional IL-1ra and TNF-alpha peak was found and these cytokines may be markers for cerebral injury.

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Authors and Affiliations

  1. Department of Neurosurgery, Virchow-Klinikum, Humboldt University Medical School, Berlin, Federal Republic of Germany

    C. Woiciechowsky M.D., D. Nestler, F. Glöckner, S. Vogel & W. R. Lanksch

  2. Department of Dermatology, Humboldt University Medical School, Berlin, Federal Republic of Germany

    K. Asadullah

  3. Department of Paediatrics, Humboldt University Medical School, Berlin, Federal Republic of Germany

    P. N. Robinson

  4. Institute of Medical Immunology, Charité, Humboldt University Medical School, Berlin, Federal Republic of Germany

    H. -D. Volk

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  1. C. Woiciechowsky M.D.
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  2. K. Asadullah
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  3. D. Nestler
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Woiciechowsky, C., Asadullah, K., Nestler, D. et al. Different release of cytokines into the cerebrospinal fluid following surgery for intra- and extra-axial brain tumours. Acta neurochir 139, 619–624 (1997). https://doi.org/10.1007/BF01411996

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