Summary
To compare the Ki-67 and Proliferating Cell Nuclear Antigen (PCNA), markers of cell proliferation, paraffin embedded surgical specimens from 56 human astrocytic tumours (8 pilocytic tumours, grade I; 9 low (II) grade and 9 anaplastic (grade III) astrocytomas and 30 glioblastomas, grade IV) were immunolabelled with the anti-PCNA (PC 10, DAKO) and anti-Ki-67 (DAKO) antibodies. For the latter immunostaining the microwave oven processing was performed. The Ki-67 and PCNA labelling indices (LIs) were statistically compared. For the majority of cases, PCNA LI was higher than that obtained with anti-Ki-67 antibody and the intensity of staining for PCNA was more variable. The statistically significant difference of the percentage of PCNA LIs was found only between low grade (I and II) and high grade (III and IV) tumours, while Ki-67 LIs discriminates each group of glial tumours; thus this latter marker is more sensitive and specific.
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Allegranza A, Girlando S, Arrigoni GL, Veronese S, Mauri FA, Gambacorta M, Pollo B, Dalla Palma P, Barbareschi M (1991) Proliferating cell nuclear antigen expression in central nervous system neoplasms. Virchows Arch 419: 417–423
Bravo R, Frank R, Blundell PA, Macdonald-Bravo H (1987) Cyclin/PCNA is the auxiliary protein of DNA polymerase-delta. Nature 326: 515–517
Bravo R, Macdonald-Bravo H (1987) Existence of two populations of cyclin proliferating cell nuclear antigen during the cell cycle: association with DNA replication sites. J Cell Biol 105: 1549–1554
Gerdes J, Lemke H, Baisch H, Wacker HH, Schwab U, Stein H (1984) Cell cycle analysis of a proliferation associated human nuclear antigen defined by the monoclonal antibody Ki-67. J Immunol 133: 710–715
Golick ML, Rice M (1992) Optimum staining of PCNA in paraffin sections is dependent on fixation, drying, and intensification. J Histotechnol 5: 39–41
Hall PA, Levison DA, Woods AL, Yu CC-W, Kellock DB, Watkins JA, Barnes DM, Gillett CE, Camplejon R, Dover R, Waseem NH, Lane DP (1990) Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections: an index of cell proliferation with evidence of deregulated expression in some neoplasms. J Pathol 162: 285–294
Haapasalo H, Isola J, Sallinen P, Kalimo H, Helin H, Rantala I (1993) Aberrant p53 expression in astrocytic neoplasms of the brain: association with proliferation. Am J Pathol 142: 1347–1351
Hoshimo T, Rodriquez LA, Cho KG, Lee KS, Wilson CB, Edwards MSB, Levin VA, Davis RL (1988) Prognostic implications of the proliferative potential of low-grade astrocytomas. J Neurosurg 69: 839–842
Hoshino T, Prados M, Wilson CB, Cho KG, Lee KS, Davis RL (1989) Prognostic implications of the bromodeoxyuridine labeling index in human gliomas. J Neurosurg 71: 335–341
Jaros E, Perry RH, Adam L, Kelly PJ, Crawford PJ, Kalbag RM, Mendelow AD, Sengupta RP, Pearson ADJ (1992) Prognostic implications of p53 protein, epidermal growth factor receptor, and Ki-67 labelling in brain tumours. Br J Cancer 66: 373–385
Kamb A, Gruis NA, Weaver-Feldhaus J, Liu Q, Harshman K, Tavtigian SV, Stockert E, Day RS, Johnson BE, Skolnick MH (1994) A cell cycle regulator potentially involved in genesis of many tumor types. Science 264: 436–440
Karamitopoulou E, Perentes E, Melachrinou M, Maraziotis T (1993) Proliferating cell nuclear antigen immunoreactivity in human central nervous system neoplasms. Acta Neuropathol (Berl) 85: 316–322
Karamitopoulou E, Perentes E, Diamantis I, Maraziotis T (1994) Ki-67 immunoreactivity in human central nervous system tumors: a study with MIB 1 monoclonal antibody on archival material. Acta Neuropathol (Berl) 87: 47–54
Khoshyomn S, Maier H, Morimura T, Kitz K, Budka H (1993) Immunostaining for proliferating cell nuclear antigen: its role in determination of proliferation in routinely processed human brain tumor specimens. Acta Neuropathol (Berl) 86: 582–589
Korkolopoulou P, Christodoulou P, Papanikolaou A, Thomas-Tsagli E (1993) Proliferating cell nuclear antigen and nucleolar organizer regions in CNS tumors: correlation with histological type and tumor grade. Am J Surg Pathol 17: 912–919
Kurki P, Vanderlaan M, Dolbeare F, Gray J, Tan EM (1986) Expression of proliferating cell nuclear antigen (PCNA)/cyclin during the cell cycle. Exp Cell Res 166: 209–219
Louis DN, Edgerton S, Thor AD, Hedley-Whyte ET (1991) Proliferating cell nuclear antigen and Ki-67 immunohistochemistry in brain tumors: a comparative study. Acta Neuropathol (Berl) 81: 675–679
Onda K, Davis RL, Wilson CB (1994) Regional differences in bromodeoxyuridine uptake, expression of Ki-67 protein, and nucleolar organizer region counts in glioblastoma multiforme. Acta Neuropathol (Berl) 87: 586–593
Schiffer D, Chio A, Giordana MT, Pezzulo T, Vigliani MC (1993) Proliferating cell nuclear antigen expression in brain tumors, and its prognostic role in ependymomas: an immunohistochemical study. Acta Neuropathol (Berl) 85: 495–502
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Kordek, R., Biernat, W., Alwasiak, J. et al. Proliferating cell nuclear antigen (PCNA) and Ki-67 immunopositivity in human astrocytic tumours. Acta neurochir 138, 509–513 (1996). https://doi.org/10.1007/BF01411168
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DOI: https://doi.org/10.1007/BF01411168