Summary
The total activities of lactate dehydrogenase (LD) and the LD isoenzyme distribution were determined on homogenates from 61 benign and malignant intracranial tumours, and in serum and cerebrospinal fluid from 45 and 28 of the tumour patients respectively.
The LD activity and the LD isoenzyme distribution in serum were not changed in patients with intracranial tumours. The LD activity in the cerebrospinal fluid was raised in about 50% of the tumour patients, but without any significant differences between patients with benign and malignant tumours.
Except for very low activities in acoustic schwannomas and in pituitary adenomas, the tumour tissue did not differ significantly from normal brain in total LD activity. The LD isoenzyme distribution showed a pronounced cathodal shift in the grade 3–4 astrocytomas and the metastatic carcinomas, but an anodal shift in the grade 1–2 astrocytomas and the oligodendrogliomas.
The meningiomas and the acoustic schwannomas showed a midzone isoenzyme pattern with a dominant LD3 fraction.
Knowledge of the LD isoenzyme distribution can thus sometimes be a complement to the histological examination in the classification of brain tumours.
Similar content being viewed by others
References
Braun, H., Ley, H., Quantitative Bestimmung der GPT, GOT und LDH im Liquor. Münch. med. Wschr.32 (1966), 1562–1566.
Buckell, M. M., Crompton, R., Robertson, M. C., Barnes, G. K., Lactate dehydrogenase in cerebral cyst fluids. J. Neurosurg.32 (1970), 545–552.
Davies-Jones, G. A. B., Lactate dehydrogenase and glutamic oxalacetic transaminase of the cerebrospinal fluid in tumours of the central nervous system. J. Neurol. Neurosurg. Psychiat.32 (1969), 324–327.
Delank, H. W., Engelmann, G., Die LDH und deren Isoenzyme im Liquor cerebrospinalis. Dtsch. Z. Nervenheilk.187 (1965), 256–264.
Edebo, L., A new press for the disruption of micro-organisms and other cells. J. Biochem. Microbiol. Technol. Eng.2 (1960), 453–479.
Fleisher, G. A., Wakim, K. G., Goldstein, N. P., Glutamic-oxalacetic transaminase and lactic dehydrogenase in serum and cerebrospinal fluid of patients with neurologic disorders. Staff Meet. Mayo Clin.32 (1957), 188–197.
Gerhardt, W., Clausen, J., Christensen, E., Riishede, J., Changes of LDH-isoenzymes, esterases, acid phosphatases and proteins in malignant and benign human brain tumours. Acta Neurol. Scand.39 (1963), 85–111.
— — — —, Lactate dehydrogenase isoenzymes in the diagnosis of human benign and malignant brain tumours. J. Nat. Cancer Inst.38 (1967), 343–357.
—, Petri, C., Distribution of lactate dehydrogenase isozymes of human brain tissue. Acta Neurol. Scand.41, suppl. 13 (1965), 609.
Gobiet, W., Der diagnostische Wert der LDH-Bestimmung und ihrer Isoenzyme bei intrakraniellen Prozessen. Z. Neurol.202 (1972), 247–250.
Goldman, R. D., Kaplan, N. O., Hall, T., Lactic dehydrogenase in human neoplastic tissues. Cancer Res.24 (1964), 389–398.
Green, J. B., Oldewurtel, H. A., Forster, F. M., Glutamic oxalacetic transaminase (GOT) and lactic dehydrogenase (LDH) activities. Neurology (Minneap.)32 (1959), 540–544.
Haglid, K., Carlsson, C.-A., Thulin, C.-A., Lactate dehydrogenase isoenzymes and proteins in human gliomas. Neurochirurgia13 (1970), 19–27.
Heller, W., Oldenkott, P., Klimsch-chemische Diagnostik bei Hirntumoren und Hirnverletzungen. Deutsch. Med. Wochschr.96 (1971), 1222–1224.
Heller, W., Oldenkott, P., Driessen, W., Elies, W., Blankenhorn, H., Klinischchemische Untersuchungen zur Früherfassung von bösartigen Hirntumoren. Ärztl. Forsch.25 (1971), 44–47.
Hildebrand, J., Levin, S., Enzymatic activities in cerebrospinal fluid in patients with neurological diseases. Acta Neurol. Belg.73 (1973), 229–240.
Kernohan, J. W., Sayre, G. P., Tumors of the central nervous system. AFIP atlas of tumor pathology. 1st series, fascicle 35, Armed Forces Institute of Pathology, Washington D.C., 1952.
Lindblom, U., Vrethammar, T., Aberg, B., Isoenzymer av mjölksyredehydrogenas vid hjärnskada. Nord. Med.77 (1967), 337–340.
Lowry, O. H., Rosenbrough, N. J., Farr, A. L., Randall, R. J., Protein measurement with the folin phenol reagent. J. Biol. Chem.193 (1951), 265–275.
Löwenthal, A., van Sande, M., Karcher, D., Heterogenity of lactic and malic dehydrogenase in serum, cerebrospinal fluid, and brain extracts in man and sheep. Ann. N.Y. Acad. Sci.94 (1961), 988–995.
Magnusson, K. E.,Physical studies on the freeze-pressing of biological material. Diss., Linköping 1975.
Pfleiderer, G., Wachsmuth, E. D., Alters- und funktionsabhängige Differenzierung der Lactatdehydrogenase menschlicher Organe. Biochem. Zschr.334 (1961), 185–198.
Rabow, L., Isoenzymes of lactic dehydrogenase as a prognosticon in patients with contusio cerebri. Scand. J. Rehab. Med.4 (1972), 90–92.
- Lactate dehydrogenase, hydroxybutyrate dehydrogenase and LD-isoenzymes in brain tissues. Acta Neurochir., in press.
- Tibbling, G., Hydroxybutyrate dehydrogenase (HBP), lactate dehydrogenase (LD), and LD isoenzymes in serum after cranio-cerebral injury. Acta Neurochir., in press.
Rubinstein, L. J., Tumors of the central nervous system. AFIP atlas of tumor pathology. 2nd series, fascicle 6, Armed Forces Institute of Pathology, Washington D.C., 1972.
Sherwin, A. L., le Blanc, F. E., McCann, W. P., Altered LDH-isozymes in brain tumours. Arch. Neurol.18 (1968), 311–315.
Spolter, H., Thompson, H. G., Jr., Factors affecting lactic dehydrogenase and glutamic oxalacetic transaminase activities in cerebrospinal fluid. Neurology (Minneap.)12 (1962), 53–59.
van der Helm, H. J., Isoenzymes in different parts of the brain. J. Neurochem.9 (1962), 325.
Vara-Lopez, R., Vara-Thorbeck, R., Modification in the activity of some enzymes of the cerebrospinal fluid in patients with intracranial tumors. J. Neurosurg.34 (1971), 749–752.
Warburg, O., On the origin of cancer cells. Science123 (1956), 309.
Wróblewski, F., Decker, B., Wróblewski, R., The clinical implications of spinal fluid lactic dehydrogenase activity. N. Eng. J. Med.258 (1958) 635–639.
—, La Due, J. S., Lactic dehydrogenase activity in blood. Proc. Soc. Exp. Biol. Med.90 (1955), 210–213.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rabow, L., Kristensson, K. Changes in lactate dehydrogenase isoenzyme patterns in patients with tumours of the central nervous system. Acta neurochir 36, 71–81 (1977). https://doi.org/10.1007/BF01405988
Issue Date:
DOI: https://doi.org/10.1007/BF01405988