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Direct gene transfer via polyethylene glycol

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Journal of tissue culture methods

Summary

Polyethylene glycol can be used to induce DNA uptake into plant protoplasts. Procedures for isolation, culture and transformation ofN. tabacum protoplasts are described and can be adapted for other dicot and monocot species. Criteria for proof of transformation are discussed.

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Authors and Affiliations

  1. Agricultural Biotechnology Research Unit, Ciba-Geigy Corporation, Research Triangle Park, 27709, North Carolina

    C. M. Johnson, G. K. Carswell & R. D. Shillito

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  1. C. M. Johnson
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  2. G. K. Carswell
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  3. R. D. Shillito
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Johnson, C.M., Carswell, G.K. & Shillito, R.D. Direct gene transfer via polyethylene glycol. Journal of Tissue Culture Methods 12, 127–133 (1989). https://doi.org/10.1007/BF01404438

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