Summary
Actin microfilaments in isolated root-tip cells from wheat (Triticum aestivum L. cv. Kite) were visualized by immunofluorescence microscopy using two different antiactin monoclonal antibodies. Cells in interphase contain predominantly subcortical bundles of microfilaments, as described in many cell types, but in preprophase and prophase cells, immunodetectable actin is organized solely in ordered arrays of cortical microfilaments that cover the entire surface of the cell, transverse on lateral faces, random on end walls. Intermediate stages with random and transverse microfilaments are also seen on lateral faces. The cell cycle stage-dependent transverse cortical microfilaments described here are previously unreported in higher plant cells.
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Abbreviations
- Ig:
-
immunoglobulin
- MF:
-
microfilament
References
Clayton L, Lloyd CW (1985) Actin organization during the cell cycle in meristematic plant cells. Exp Cell Res 156: 231–238
Kakimoto T, Shibaoka H (1987) Actin filaments and microtubules in the preprophase band and phragmoplast of tobacco cells. Protoplasma 140: 151–156
Lessard JA (1988) Two monoclonal antibodies to actin: one muscle selective and one generally reactive. Cell Motil 10: 349–362
Marc J, Gunning BES (1988) Monoclonal antibodies to a fern spermatozoid detect novel components of the mitotic and cytokinetic apparatus in higher plant cells. Protoplasma 142: 15–24
Palevitz BA (1987) Actin in the preprophase band ofAllium cepa. J Cell Biol 104: 1515–1519
Parthasarathy MV, Perdue TD, Witztum A, Alvernaz J (1985) Actin network as a normal component of the cytoskeleton in many vascular plant cells. Am J Bot 72: 1318–1323
Schmit AC, Lambert AM (1987) Characterization and dynamics of cytoplasmic F-actin in higher plant endosperm cells during interphase, mitosis, and cytokinesis. J Cell Biol 105: 2157–2166
Seagull RW, Falconer MM, Weerdenburg CA (1987) Microfilaments: dynamic arrays in higher plant cells. J Cell Biol 104: 995–1004
Traas JA, Doonan JH, Rawlins DJ, Shaw PJ, Watts J, Lloyd CW (1987) An actin network is present in the cytoplasm throughout the cell cycle of carrot cells and associated with the dividing nucleus. J Cell Biol 105: 387–395
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McCurdy, D.W., Sammut, M. & Gunning, B.E.S. Immunofluorescent visualization of arrays of transverse cortical actin microfilaments in wheat root-tip cells. Protoplasma 147, 204–206 (1988). https://doi.org/10.1007/BF01403349
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DOI: https://doi.org/10.1007/BF01403349