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Expression of cALLa/NEP on gliomas: A possible marker of malignancy

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Summary

First described on pre-B leukemia cells, the common acute lymphoblastic leukemia antigen (cALLa) is also expressed on glioma cellsin vitro. Its identity to neutral endopeptidase (NEP) (E.C.3.24.11) was corroborated by our finding that cALLa positive glioma cells had NEP activity. To study cALLa/NEP distribution on glial tumours in vivo, we examined 76 brain tumour biopsies by immunostaining techniques on frozen tissue sections using anticALLa (FAH99) and anti-NEP (135 A 3) monoclonal antibodies. We found that 96% of grade 4 gliomas (25/26) expressed NEP. Whereas only 45% (4/9) of grade 3 or anaplastic astrocytomas did. In low grade gliomas, we found 2 positive tumours out of 21 tested (10%). Double immunostaining procedures revealed that NEP was co-expressed with GFAP. However no NEP could be detected on non-glial brain tumours nor on reactive astrocytes. These results suggest that cALLa/NEP expression could be linked to malignant progression of gliomas.

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Authors and Affiliations

  1. Neurosurgical Department, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland

    L. Monod, M. F. Hamou & N. de Tribolet

  2. Hopital Tenon, Institut National de la Santé et de la Recherche Médicale U 64, Paris, France

    P. Ronco & P. Verroust

Authors
  1. L. Monod
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  2. M. F. Hamou
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  3. P. Ronco
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  4. P. Verroust
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  5. N. de Tribolet
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Monod, L., Hamou, M.F., Ronco, P. et al. Expression of cALLa/NEP on gliomas: A possible marker of malignancy. Acta neurochir 114, 3–7 (1992). https://doi.org/10.1007/BF01401105

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