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Detection by immunogold labelling of P75 readthrough protein near an extremity of beet necrotic yellow vein virus particles

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Summary

RNA 2 of beet necrotic yellow vein virus carries the cistron for the 21 kd coat protein at its 5′-extremity. During translation, the coat protein cistron termination codon is suppressed about 10% of the time so that translation continues into the adjacent open reading frame to produce a 75 kd species, known as P75, which contains the coat protein sequence at its N-terminus. Immunoblotting experiments with a P75-specific antiserum showed that P75 is present in only trace amounts in purified virus preparations. Electron microscopic visualization of immunogold-labelled virions in crude tissue extracts has provided evidence for an association between P75 and at least a fraction of the BNYVV particles, with P75 being predominantly located near one end of the rod-shaped virions. This finding is discussed in the context of the current model for the role of P75 in virus assembly and vector transmission.

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Authors and Affiliations

  1. Station Grandes Cultures, Laboratoire de Pathologie Végétale, INRA, Colmar Cedex

    Anne-Marie Haeberlé

  2. Institut de Biologie Moléculaire des Plantes du Centre National de la Recherche Scientifique et de l'Université Louis Pasteur, Strasbourg Cedex

    Christiane Stussi-Garaud, Corinne Schmitt, K. E. Richards, H. Guilley & G. Jonard

  3. INSERM, Strasbourg, France

    J. -C. Garaud

Authors
  1. Anne-Marie Haeberlé
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  2. Christiane Stussi-Garaud
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  3. Corinne Schmitt
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  4. J. -C. Garaud
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  5. K. E. Richards
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  6. H. Guilley
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  7. G. Jonard
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Haeberlé, AM., Stussi-Garaud, C., Schmitt, C. et al. Detection by immunogold labelling of P75 readthrough protein near an extremity of beet necrotic yellow vein virus particles. Archives of Virology 134, 195–203 (1994). https://doi.org/10.1007/BF01379118

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  • DOI: https://doi.org/10.1007/BF01379118

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