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The formation of aplastidic abscission (tmema) cells and protonemal disruption in the mossBryum tenuisetum Limpr. is associated with transverse arrays of microtubules and microfilaments

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Summary

When grown on nutrient agar, protonemata ofBryum tenuisetum produce aerial filaments containing several abscission or tmema cells (TC). Basipetal migration of the nucleus and some of the chloroplasts signals the onset of TC formation. This is followed by the creation of a plastid-free zone at the base of the mother cell. The ensuing cytokinesis produces a very short aplastidic TC. This expands without the deposition of new wall material. Eventually the wall ruptures around the equator thus disrupting the protonemal filament. The site of wall breakdown is marked by a narrow band of cortical cytoplasm containing colocalized circumferential rings of actin filaments and microtubules. A transverse band of microtubules appears at the extreme basal end of the tmema mother cell. This band, which is not colocalized with actin filaments, migrates distally over the surface of the nucleus. Intimate spatial and developmental correlations suggest that this transverse array of the microtubules has a key role in excluding plastids from the TC. It is therefore considered not to be homologous with a preprophase band.

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Abbreviations

NC:

proximally-situated non-sister neighbour cell of a tmema cell

MT:

microtubules

PPB:

preprophase band

SC:

distally situated sister cell of a tmema cell

TC:

tmema cell

TMC:

tmema mother cell

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Author notes

  1. F. Alfano

    Present address: Dipartimento di Biologia Vegetale, Università di Napoli, Napoli, Italy

Authors and Affiliations

  1. Department of Biology, Royal Holloway and Bedford New College, Egham, Surrey

    J. A. Goode & A. D. Stead

  2. School of Biological Sciences, Queen Mary and Westfield College, Mile End Road, El 4NS, London, UK

    F. Alfano & J. G. Duckett

Authors
  1. J. A. Goode
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  2. F. Alfano
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  3. A. D. Stead
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  4. J. G. Duckett
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Goode, J.A., Alfano, F., Stead, A.D. et al. The formation of aplastidic abscission (tmema) cells and protonemal disruption in the mossBryum tenuisetum Limpr. is associated with transverse arrays of microtubules and microfilaments. Protoplasma 174, 158–172 (1993). https://doi.org/10.1007/BF01379048

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  • DOI: https://doi.org/10.1007/BF01379048

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