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Role of cucumber mosaic virus and its satellite RNA in the etiology of tomato fruit necrosis in Italy

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Summary

A cucumber mosaic virus (CMV) isolate supporting a natural 390-ribonucleotide satellite was used to reproduce under experimental conditions a disease of processing tomatoes called fruit necrosis. The virus induced incomplete differentiation of the vascular tissue of fruit stalks, which was the likely cause of the disease. On the other hand, the satellite RNA attenuated viral symptoms on tomato leaves reproducing the disease pattern typically observed in the field. The biological properties of this seemingly new variant of cucumoviral satellite RNAs were determined.

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References

  1. Benetti MP (1988) La necrosi dei frutti di pomodoro. Inf Fitopatol 39: 21–24

    Google Scholar 

  2. Blancard D (1988) Maladies de la tomate: Observer, Identifier, Lutter. Rev Hort INRA

  3. Collmer CW, Howell SH (1992) Role of satellite RNA in the expression of symptoms caused by plant viruses. Annu Rev Phytopathol 30: 419–442

    Google Scholar 

  4. Crescenzi A, Barbarossa L, Gallitelli D, Martelli GP (1993) Cucumber mosaic cucumovirus populations in Italy under natural epidemic conditions and after a satellite-mediated protection test. Plant Dis 77: 28–33

    Google Scholar 

  5. Crescenzi A, Grieco F, Gallitelli D (1992 a) Nucleotide sequence of a satellite RNA of a strain of cucumber mosaic virus associated with tomato fruit necrosis. Nucleic Acids Res 20: 2886

    PubMed  Google Scholar 

  6. Devic M, Jaegle M, Boulcombe D (1990) Cucumber mosaic virus satellite RNA (strain Y): analysis of sequences which affect systemic necrosis on tomato. J Gen Virol 71: 1443–1449

    PubMed  Google Scholar 

  7. Gallitelli D, Di Franco A, Vovlas C, Kaper JM (1988) Infezioni miste del virus del mosaico del cetriolo (CMV) e di potyvirus in colture ortive di Puglia e Basilicata. Inf Fitopatol 38: 57–64

    Google Scholar 

  8. Grieco F, Cillo F, Barbarossa L, Gallitelli D (1992) Nucleotide sequence of a cucumber mosaic virus satellite RNA associated with a tomato top stunting. Nucleic Acids Res 20: 6733

    PubMed  Google Scholar 

  9. Horns T, Jeske H (1991) Localization of abutilon mosaic virus (AbMV) DNA within leaf tissue by in situ hybridization. Virology 181: 580–588

    PubMed  Google Scholar 

  10. Johansen DA (1940) Plant microtechnique. McGraw-Hill, New York

    Google Scholar 

  11. Jordà C, Alfaro A, Aranda MA, Moriones E, Garcia-Arenal F (1992) Epidemic of cucumber mosaic virus plus satellite RNA in tomatoes in Eastern Spain. Plant Dis 76: 363–366

    Google Scholar 

  12. Kaper JM (1992) Satellite-induced viral symptom modulation in plants: a case of nested parasitic nucleic acids competing for genetic expression. Res Virol 143: 5–10

    PubMed  Google Scholar 

  13. Kaper JM, Tousignant ME, Thompson SM (1981) Cucumber mosaic virus associated-RNA 5. VIII. Identification and partial characterization of a CARNA 5 incapable of inducing tomato necrosis. Virology 114: 526–533

    Google Scholar 

  14. Kaper JM, Waterworth HE (1981) Cucumoviruses. In: Kurstak E (ed) Handbook of plant virus infections and comparative diagnosis. Elsevier, Amsterdam pp 257–332

    Google Scholar 

  15. Lot H, Marrou J, Quiot JB, Esvan CH (1972) Contribution à l'étude du virus de la mosaique du concombre (CBV). II. Méthode rapide de purification du virus. Ann Phytopathol 4: 25–38

    Google Scholar 

  16. Martelli GP, Russo M (1985) Virus-Host relationships. Symptomatological and ultrastructural aspects. In: Francki RIB (ed) Polyhedral virions with tripartite genomes. Plenum Press, New York (The Plant Viruses, vol 1, pp 163–205)

    Google Scholar 

  17. Owen J, Palukaitis P (1988) Characterization of cucumber mosaic virus I. Molecular heterogeneity mapping of RNA 3 in eight CMV strains. Virology 166: 495–502

    PubMed  Google Scholar 

  18. Palukaitis P, Roossinck MJ, Dietzgen RG, Francki RIB (1992) Cucumber mosaic virus. Adv Virus Res 41: 281–348

    PubMed  Google Scholar 

  19. Piazzolla P, Diaz-Ruiz JR, Kaper JM (1979) Nucleic acid homologies of eighteen cucumber mosaic virus isolates determined by competition hybridization. J Gen Virol 45: 361–369

    Google Scholar 

  20. Roossinck MJ, Sleat D, Palukaitis P (1992) Satellite RNAs of plant viruses: structure and biological effects. Microbiol Rev 56: 265–279

    PubMed  Google Scholar 

  21. Rosenbaum V, Riesner D (1987) Temperature-gradient gel electrophoresis. Thermodynamic analysis of nucleic acids and proteins in purified form and in cellular extracts. Biophys Chem 26: 235–246

    PubMed  Google Scholar 

  22. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: A laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor

    Google Scholar 

  23. Seed B (1982) Diazotizable arylamine cellulose paper for the coupling and hybridization of nucleic acid. Nucleic Acids Res 10: 1779–1810

    Google Scholar 

  24. Tien P, Steger G, Rosenbaum V, Kaper JM, Riesner D (1987) Double-stranded cucumovirus associated RNA 5: experimental analysis of necrogenic and non-necrogenic variants by temperature-gradient gel electrophoresis. Nucleic Acids Res 15: 5069–5083

    PubMed  Google Scholar 

  25. White JL, Kaper JM (1989) A simple method for detection of viral satellite RNAs in small plant tissue sample. J Virol Methods 23: 83–94

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Istituto di Patologia Vegetale, Portici

    A. Crescenzi

  2. Dipartimento di Protezione delle Piante dalle Malattie and Centro di Studio del CNR sui virus e le virosi delle colture mediterranee, Bari

    L. Barbarossa, A. Di Franco & D. Gallitelli

  3. Tecnogen SCpA, Parco Scientifico, Piana di Monte Verna

    F. Cillo

  4. Laboratorio di Nematologia Agraria del C.N.R., Bari, Italy

    N. Vovlas

Authors
  1. A. Crescenzi
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  2. L. Barbarossa
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  3. F. Cillo
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  4. A. Di Franco
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  5. N. Vovlas
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  6. D. Gallitelli
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Crescenzi, A., Barbarossa, L., Cillo, F. et al. Role of cucumber mosaic virus and its satellite RNA in the etiology of tomato fruit necrosis in Italy. Archives of Virology 131, 321–333 (1993). https://doi.org/10.1007/BF01378635

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  • DOI: https://doi.org/10.1007/BF01378635

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