Abstract
7-Hydroxycoumarin (7-HC) is a naturally occuring substance with a variety of biological activities, e.g. antitumoral and immunomodulatory action. the potential mechanisms of 7-HC action on tumor cells have been investigated in a model of mouse fibroblasts transformed by distinet retroviral vectors carrying the oncogenesmyc andras. Treatment of the different oncogenic transformants with 7-HC in non-cytotoxic concentrations causes a strong inhibition of their proliferative capacity as measured by growth curves. Furthermore, incubation of theras andmyc transformants with 7-HC abrogated soft agar colony formation when compared to untreated cultures. These changes in cell growth characteristics of the various oncogene-transformed mouse fibroblasts were transient, since removal of 7-HC from the cultures caused a reappearance of the transformed properties of these cells within 6 days. The 7-HC-mediated inhibition of the proliferative capacity of the transformants was preceded by a selective down-regulation of oncogene expression. These data suggest that 7-HC causes a reversible inhibition ofras- andmyc-induced neoplastic properties in transformed fibroblasts by selective down-regulation of retroviral RNA levels.
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Abbreviations
- 7-HC:
-
7 hydroxycoumarin
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Seliger, R., Pettersson, H. 7-Hydroxycoumarin inhibits oncogene-induced transformation of murine fibroblasts. J Cancer Res Clin Oncol 120 (Suppl 1), S23–S27 (1994). https://doi.org/10.1007/BF01377119
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DOI: https://doi.org/10.1007/BF01377119