Summary
We analyzed the distribution of fibronectin in routinely embedded tissue specimens from 53 skin wounds and 6 postmortem wounds. In postmortem wounds a faint but focal positive staining was exclusively found at the margin of the specimens which dit not extend into the adjacent stroma. Vital wounds were classified into 3 groups. The first comprising lesions with wound ages ranging from a few seconds to 30 min, the second comprising those with wound ages upt to 3 weeks, and the third group with lesions more than 3 weeks old. Ten out of 17 lesions with a wound age up to 30 min showed a clear positive reaction within the wound area. Three specimens in this group were completely negative, while in 4 additional cases the result was not significantly different from postmortem lesions. These 7 cases were characterized by acute death with extremely short survival times (only seconds). In wounds up to 3 weeks old fibronectin formed a distinct network containing an increasing number of inflammatory cells corresponding to the wound age. In 2 cases with a survival time of 17 days and in all wounds older than 3 weeks fibronectin was restricted to the surface of fibroblasts and to parallel arranged fibers in the granulation tissue without any network structures. We present evidence that fibronectin is a useful marker for vital wounds with a survival time of more than a few minutes. Fibronectin appears before neutrophilic granulocytes migrate into the wound area. Since a faint positive fibronectin staining is seen in postmortem lesions and bleedings, we propose that only those wounds which show strong positive fibronectin staining also extending into the adjacent stroma should be regarded as vital.
Zusammenfassung
Es wurden 53 vitale Hautwunden mit einem Wundalter von wenigen Sekunden/Minuten
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References
Alitalo K, Hovi T, Vaheri A (1980) Fibronectin is produced by human macrophages. J Exp Med 147:1779–1791
Berg S, Ditt J, Friedrich D, Bonte W (1968) Möglichkeiten der biochemischen Wundaltersbestimmung. Dtsch Z Gerichtl Med 63:183–198
Berg S, Elbel R (1969) Altersbestimmung subcutaner Blutungen. Muench Med Wochenschr 111:1185–1190
Berg S (1975) Vitale Reaktionen und Zeitschätzungen. In: Mueller B (eds) Gerichtliche Medizin, vol 1. Springer, Berlin Heidelberg New York, pp 326–340
Betz P, Penning R, Eisenmenger W (1991) Lipophagen der Haut als zusatzlicher Parameter für die histologische Wundalterschatzung. Rechtsmedizin 1:139–144
Boehm E, Tschomakov M (1973) Ein Sekundenphänomen der vitalen Reaktion. Beitr Gerichtl Med 31:221–229
Clark RAF, DellaPelle P, Manseau E, Lanigan JM, Dvorak HF, Colvin RB (1982) Blood vessel fibronectin increases in conjunction with endothelial cell proliferation and capillary ingrowth during wound healing. J Invest Dermatol 79:269–276
Clark RAF, Lanigan JM, DellaPelle P, Manseau E, Dvorak HF, Colvin RB (1982) Fibronectin and fibrin provide a provisional matrix for epidermal cell migration during wound reepithelialization. J Invest Dermatol 79:264–269
Davison P, Karasek M (1980) Serial cultivation of human dermal vessel endothelium: role of serum and fibronectin. Clin Res 28:566a
Eisenmenger W, Nerlich A, Gluck G (1988) Die Bedeutung des Kollagens bei der Wundaltersbestimmung. Z Rechtsmed 100:79–100
Fechner G, Hernandez M, Bajanowski T, Sepulchre MA, Brinkmann B (1992) Immunohistochemical alterations after muscle trauma. Int J Leg Med (in press)
Grinnell F, Feld M, Minter D (1980) Fibroblast adhesion to fibrinogen and fibrin substrata: requirement for cold-insoluble globulin (plasma fibronectin) Cell 19:517–525
Grinnell F, Billingham RE, Burgess L (1981) Distribution of fibronectin during wound healing in vivo. J Invest Dermatol 76:181–189
Hormann H, Seidl M (1980) Affinity chromatography on immobilized fibrin monomer, III. The fibrin affinity center of fibronectin. Hoppe Seylers Z Physiol Chem 361:1449–1452
Hormann H (1982) Fibronectin-mediator between cells and connective tissue. Klin Wochenschr 60:1265–1277
Hsu SM, Raine L, Fanger HC (1981) A comparative study of the peroxidase-antiperoxidase method and an avidin-biotin complex method for studying polypeptide hormones with radio immunoassay antibodies. Am J Clin Pathol 75:734–739
Jaffee EA, Mosher DF (1978) Synthesis of fibronectin by cultured human endothelial cells. J Exp Med 147:1779–1791
Jarecki R, Arndt U, Schultz C, Klein H (1969) Zur Unterscheidung vitaler und postmortaler Wunden durch Bestimmung des Esterasemusters der Haut. Dtsch Z Ges Gerichtl Med 66:161–169
Jilek F, Hörmann H (1979) Fibronectin (cold insoluble globulin), VI. Influence of heparin and hyaluronic acid on the binding of native collagen. Hoppe Seylers Z Physiol Chem 360: 597–603
Kurkinen M, Vaheri A, Roberts PJ, Steinman S (1980) Sequential appearance of fibronectin and collagen in experimental granulation tissue. Lab Invest 43:47–57
Linder E, Stenman S, Lehto V-P, Vaheri A (1978) Distribution of fibronectin in human tissues and relationship to other connective tissue components. Ann NY Acad Sci 312:151–159
Mosher DF (1975) Cross linking of cold insoluble globulin by fibrin stabilizing factor. J Biol Chem 250:6614–6621
Mosesson MW, Umfleet RA (1970) The cold-insoluble globulin of human plasma. 1. Purification, primary characterization, and relationship to fibrinogen and other cold-insoluble fraction components. J Biol Chem 245:5728–5736
Nerlich A, Berndt R, Schleicher E (1991) Differential basement membrane composition in multiple epitheloid haeman gioendotheliomas of liver and lung. Histopathology 18:303–307
Niewiarowski St, Regoezi E, Mustard JF (1972) Adhesion of fibroblasts to polymerizing fibrin and retraction of fibrin induced by fibroblasts. Proc Soc Exp Biol Med 140:199–204
Oehmichen M, Schmidt V, Stuka K (1989) Freisetzung von Proteinase-Inhibitoren als vitale Reaktion im frühen posttraumatischen Intervall. Z Rechtsmed 102:461–472
Oehmichen M (1990) Die Wundheilung. Springer, Berlin Heidelberg New York
Postlethwaite AE, Keshi-Oja J, Balian G, Kang AH (1981) Induction of fibroblast chemotaxis by fibronectin. J Exp Med 153:494–499
Raekallio J (1965) Die Altersbestimmung mechanisch bedingter Hautwunden mit enzymhistochemischen Methoden. Schmidt Romhild, Löbeck
Raekallio J (1972) Determination of the age of wounds by histochemical and biochemical methods. Forensic Sci 1:3–6
Repesh LA, Fitzgerald TJ, Furcht LT (1982) Fibronectin involvement in granulation tissue and wound healing in rabbits. J Histochem Cytochem 30:351–358
Ruoslahti E, Vaheri A (1974) Novel human serum protein from fibroblast plasma membrane. Nature 248:789–791
Ruoslahti E, Vaheri A (1975) Interaction of soluble fibroblast surface antigen with fibrnogen and fibrin. Identity with coldinsoluble globulin of human plasma. J Exp Med 141:497–501
Schneider V (1974) Über rasterelektronenmikroskopische Untersuchungen an vital und postmortal entstandenen “Thromben”. Z Rechtsmed 74:47–54
Stenman S, Vaheri A (1978) Distribution of a major connective tissue protein, fibronectin, in normal human tissues. J Exp Med 147:1054–1064
Vaheri A, Ruoslahti R, Westermark B, Pontén J (1976) A common cell type specific surface antigen in cultured human glial cells and fibroblasts: loss in malignant cells. J Exp Med 143:64–72
Viljanto J, Penttinen R, Raekallio J (1981) Fibronectin in early phases of wound healing in children. Acta Chir Scand 147: 7–13
Yamada KM, Olden K (1978) Fibronectins: Adhesive glycoproteins of cell surface and blood. Nature 275:179–184
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This study was supported by a grant from the “Deutsche Forschungsgemeinschaft” (grant Ei 209/3-1) and by a grant from the “Friedrich-Baur-Stiftung” University of Munich
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Betz, P., Nerlich, A., Wilskel, J. et al. Immunohistochemical localization of fibronectin as a tool for the age determination of human skin wounds. Int J Leg Med 105, 21–26 (1992). https://doi.org/10.1007/BF01371232
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DOI: https://doi.org/10.1007/BF01371232