Summary
A modification to the DNA extraction method “preferential lysis” (Gill et al. 1985) is proposed which can be applied to DNA mixtures of vaginal cells and spermatozoa. In mixtures with a low sperm content the further loss of sperm DNA caused by the extraction can be avoided by using “mild preferential lysis”. Amplification by PCR (polymerase chain reaction) then yields sufficient DNA to be able to identify both components in the mixture.
Zusammenfassung
Vorgestellt wird eine Modifikation der DNA-Extraktionsmethode “Preferentielle Lyse” (Gill et al. 1985) die üblicherweise bei Vorliegen von Vaginalzell/Sperma-Mischungen angewendet wird. Bei Mischungen mit nur geringem Spermienanteil sollen extraktionsbedingte Verluste von Sperma-DNA durch Anwendung einer “milden preferentiellen Lyse” vermieden werden. Der Nachweis erfolgte durch PCR-Amplifikation der extrahierten DNA-Proben.
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Wiegand, P., Schürenkamp, M. & Schütte, U. DNA extraction from mixtures of body fluid using mild preferential lysis. Int J Leg Med 104, 359–360 (1992). https://doi.org/10.1007/BF01369558
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DOI: https://doi.org/10.1007/BF01369558