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Ultrastructure of P-protein inHevea brasiliensis during sieve-tube development and after wounding

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Summary

P-protein and the changes it undergoes after wounding of sieve tubes of secondary phloem in one- to two-year old shoots ofHevea brasiliensis has been studied using electron microscopy. The P-protein in the form of tubules with a diameter of 8–9 nm and a lumen of 2–2.5 nm occurred in differentiating sieve elements and appeared as compact bodies which consisted of small aggregates of the tubules. As the sieve elements matured, these P-protein bodies dispersed with a disaggregation of the tubules before they turned into striated fibrils, 10–11 nm in diameter. In wounding experiments, as the mature sieve elements collapsed after cutting, their striated P-protein converted into tubules. These tubules were the same in ultrastructure as the tubules in differentiating sieve elements and they often were arranged in crystalline aggregates.

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References

  • Audley BG (1966) The isolation and composition of helical protein microfibrils fromHevea brasiliensis latex. Biochem J 98: 335–341

    Google Scholar 

  • Cronshaw J (1975) P-proteins. In: Aronoff S, Dainty J, Gorham PR, Srivastava LM, Swanson CA (eds) Phloem transport. Plenum, New York, pp 79–115

    Google Scholar 

  • — (1981) Phloem structure and function. Ann Rev Plant Physiol 32: 465–484

    Google Scholar 

  • —, Esau K (1967) Tubular and fibrillar components of mature and differentiating sieve elements. J Cell Biol 34: 801–815

    Google Scholar 

  • —, Gilder J, Stone D (1973) Fine structural studies of P-protein inCucurbita, Cucumis, andNicotiana. J Ultrastruct Res 45: 192–205

    Google Scholar 

  • Dickenson PB (1965) The ultrastructure of the latex vessel ofHevea brasiliensis. In: Mullins L (ed) Proc Nat Rubb Prod Res Ass Jubilee Conf Cambridge 1964. Maclaren & Sons, London, pp 52–64

    Google Scholar 

  • Evert RF (1977) Phloem structure and histochemistry. Ann Rev Plant Physiol 28: 199–222

    Google Scholar 

  • — (1984) Comparative structure of phloem. In: What RA, Dickinson WC (eds) Contemporary problems in plant anatomy. Academic Press, London, pp 145–234

    Google Scholar 

  • Gomez JB, Yip E (1975) Microhelices inHevea latex. J Ultrastruct Res 52: 76–84

    Google Scholar 

  • Palevitz BA, Newcomb EH (1971) The ultrastructure and development of tubular and crystalline P-protein in the sieve elements of certain papilionaceous legumes. Protoplasma 72: 399–426

    Google Scholar 

  • Parthasarathy MV, Muhlethaler K (1969) Ultrastructure of protein tubules in differentiating sieve elements. Cytobiologie 1: 17–36

    Google Scholar 

  • Read SM, Northcote DH (1983) Chemical and immunological similarities between the phloem proteins of three genera of the Cucurbitaceae. Planta 158: 119–127

    Google Scholar 

  • Sabnis DD, Hart JW (1982) Microtubule proteins and P-protein. In: Bouter D, Parthier B (eds) Nucleic acids and proteins in plants I. Springer, Berlin Heidelberg New York, pp 401–437 [Pirson A, Zimmermann MH (eds) Encyclopedia of plant physiology, new series, vol 14A]

    Google Scholar 

  • Stone DL, Cronshaw J (1973) Fine structure of P-protein filaments fromRicinus communis. Planta 113: 193–206

    Google Scholar 

  • Tata SJ, Gomez JB (1980) Isolation and characterizaton of microhelices from luloids ofHevea latex. J Rubber Res Inst Malaysia 28: 67–76

    Google Scholar 

  • Thorsch J, Esau K (1988) Ultrastructural aspects of primary phloem. Sieve elements in poinsettia (Euphorbia pulcherrima, Euphorbiaceae). Int Assoc Wood Anat Bull 9: 363–373

    Google Scholar 

  • Wergin WP, Newcomb EH (1970) Formation and dispersal of crystalline P-protein in sieve elements of soybean (Glycine max L.). Protoplasma 71: 365–388

    Google Scholar 

  • Wu J, Hao B (1987 a) Protein-storing cells in secondary phloem ofHevea brasiliensis. Kexue Tongbao 32: 118–121

    Google Scholar 

  • — — (1987 b) Ultrastructure and differentiation of protein-storing cells in secondary phloem ofHevea brasiliensis stem. Ann Bot 60: 505–512

    Google Scholar 

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Wu, Jl., Hao, Bz. Ultrastructure of P-protein inHevea brasiliensis during sieve-tube development and after wounding. Protoplasma 153, 186–192 (1990). https://doi.org/10.1007/BF01354003

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  • DOI: https://doi.org/10.1007/BF01354003

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