Summary
Changes in the organization of cortical actin microfilaments during phytochrome-mediated and blue light-induced photoorientation of chloroplasts were investigated by rhodamine-phalloidin staining in protonemal cells of the fernAdiantum capillusveneris. Low- and high-fluence rate responses were induced by partial irradiation of individual cells with a microbeam of 20 μm in width. In the low-fluence rate responses to red and blue light, a circular structure composed of microfilaments was induced on the chloroplast concentrated in the irradiated region, on the side facing the plasma membrane, as already reported in the case of the low-fluence rate response induced by polarized red or blue light. Such a structure was not observed on the chloroplasts located far from the microbeam. Time-course studies revealed that the structure was induced after the chloroplasts gathered in the illuminated region and that the structure disappeared before chloroplasts moved out of this region when the microbeam was turned off. In the high-fluence rate response to blue light, chloroplasts avoided the irradiated site but accumulated in the shaded area adjacent the edges of microbeam. The circular structure made of microfilaments was also observed on the chloroplasts gathered in the area and it showed the same behavior with respect to its appearance and disappearance during a light/dark regime as in the case of the low-fluence rate response. However, no such circular structure was observed in the high-fluence rate response to red light, in which case the chloroplasts also avoided the illuminated region but no accumulation in the adjacent areas was induced. These results indicate that the circular structure composed of microfilaments may play a role in the anchorage of the chloroplast during intracellular photo-orientation.
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References
Blatt MR, Briggs WR (1980) Blue-light-induced cortical fiber reticulation concomitant with chloroplast aggregation in the algaVaucheria sessilis. Planta 147: 355–362
—, Wessells NK, Briggs WR (1980) Actin and cortical fiber reticulation in the siphonaceous algaVaucheria sessilis. Planta 147: 363–375
Grolig F, Williamson RE, Parke J, Miller C, Anderson BH (1988) Myosin and Ca2+-sensitive streaming in the algaChara: detection of two polypeptides reacting with a monoclonal anti-myosin and their localization in the streaming endoplasm. Eur J Cell Biol 47: 22–31
Haupt W (1959) Die Chloroplasten-Drehung beiMougeotia. I. Über den quantitativen und qualitativen Lichtbedarf der Schwachlichtbewegung. Planta 53: 484–501
— (1982) Light-mediated movement of chloroplasts. Annu Rev Plant Physiol 33: 205–233
— (1987) Phytochrome control of intracellular movement. In: Furuya M (ed) Phytochrome and photoregulation in plants. Academic Press, Tokyo, pp 225–237
— Thiele R (1961) Chloroplastenbewegung beiMesotaenium. Planta 56: 388–401
Heslop-Harrison J, Heslop-Harrison Y (1990) Myosin associated with the surfaces of organelles, vegetative nuclei and generative cells in angiosperm pollen grains and tubes. J Cell Sci 94: 319–325
Izutani Y, Takagi S, Nagai R (1990) Orientation movements of chloroplasts inVallisneria epidermal cells: different effects of light at low- and high-fluence rate. Photochem Photobiol 51: 105–111
Kadota A, Wada M (1989a) Photoinduction of circular F-actin on chloroplast in a fern protonemal cell. Protoplasma 151: 171–174
— — (1989b) Enzymatic isolation of protoplasts from fern protonemal cells stainable with a fluorescent brightener. Plant Cell Physiol 30: 1107–1113
—, Koyama M, Wada M, Furuya M (1984) Action spectra for polarotropism and phototropism in protonemata of the fernAdiantum capillus-veneris. Physiol Plant 61: 327–330
— Murata T, Wada M (1986) Photoorientation of chloroplasts inAdiantum gametophytes as analyzed by video-tracking system. In: Proceedings of the XVI Yamada Conference on Phytochrome and Plant Photomorphogenesis, Okazaki, pp 147
—, Kohyama I, Wada M (1989) Polarotropism and photomovement of chloroplasts in the protonemata of the fernsPteris andAdiantum: evidence for the possible lack of dichroic phytochrome in Pteris. Plant Cell Physiol 30: 523–531
Schönbohm E (1980) Phytochrome and non-phytochrome dependent blue light effects on intracellular movements in fresh-water algae. In: Senger H (ed) The blue light syndrome. Springer, Berlin Heidelberg New York, pp 69–96
Sonobe S, Shibaoka H (1989) Cortical fine actin filaments in higher plant cells visualized by rhodamine-phalloidin after pretreatment withm-maleimidobenzoyl N-hydroxysuccinimide ester. Protoplasma 148: 80–86
Tang X, Hepler PK, Scordilis SP (1989) Immunochemical and immunocytochemical identification of a myosin heavy chain polypeptide inNicotiana pollen tubes. J Cell Sci 92: 569–574
Wada M, Kadota A, Furuya M (1983) Intracellular localization and dichroic orientation of phytochrome in plasma membrane and/ or ectoplasm of a centrifuged protonema of fernAdiantum capillus-veneris L. Plant Cell Physiol 24: 1441–1447
—, Kadota A, Wada M (1985) Blue- and red-light action in photoorientation of chloroplasts inAdiantum protonemata. Planta 165: 43–50
Yatsuhashi H, Wada M (1990) High-fluence rate responses in the light-oriented chloroplast movement inAdiantum protonemata. Plant Sci 68: 87–94
—, Hashimoto T, Wada M (1987) Dichroic orientation of photoreceptors of chloroplast movement inAdiantum protonemata. Non-helical orientation. Plant Sci 51: 165–170
Zurzycki J (1980) Blue light-induced intracellular movements. In: Senger H (ed) The blue light syndrome. Springer, Berlin Heidelberg New York, pp 50–68
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Kadota, A., Wada, M. Photoinduction of formation of circular structures by microfilaments on chloroplasts during intracellular orientation in protonemal cells of the fernAdiantum capillus-veneris . Protoplasma 167, 97–107 (1992). https://doi.org/10.1007/BF01353585
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DOI: https://doi.org/10.1007/BF01353585