Summary
The giant coenocytic green algaCaulerpa is well known for its large scale amyloplast transport. The majority of chloroplasts, however, is immobilized in the cortex of the cell. By applying UV-irradiation to localized areas of the cortex chloroplasts can be induced to slowly move towards and aggregate around the irradiated spot. Chloroplast movement is blocked by cytochalasin D, but not by colchicine or the microtubule herbicide cremart. The dynein inhibitor erythro-9-[3-(2-hydroxynonyl)] adenine (EHNA) also has no effect on chloroplast movement. However, both microtubule- and dynein-specific inhibitors block movement of amyloplasts. Using the previously developed technique of microdissection followed by immunofluorescence microscopy it can be shown that, concomitant with changes in motile behavior of chloroplasts upon irradiation, actin filaments form and rearrange around the irradiation spot. It is concluded that in contrast to amyloplast movement, immobilization and movement of chloroplasts are dependent on actin but not on microtubules. Therefore, two individual motile mechanisms appear to have evolved for independent positioning and motility of the two populations of plastids in the giant coenocyteCaulerpa.
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Abbreviations
- EHNA:
-
erythro-9-[3-(2-hydroxynonyl)] adenine
- DMSO:
-
dimethylsulfoxide
- MT:
-
microtubule
- NEM:
-
N-ethylmaleimide
References
Allen RD, Weiss DG, Hayden JH, Brown DT, Fujiwake H, Simpson M (1985) Gliding movement of and bidirectional transport along single native microtubules from squid axoplasm: evidence for an active role of microtubules in cytoplasmic transport. J Cell Biol 100: 1736–1752
Blatt MR, Weisensee MH, Haupt W (1981) A light-dependent current associated with chloroplast aggregation in the algaVaucheria sessilis. Planta 152: 513–526
Calvert HE (1976) Culture studies on some Florida species ofCaulerpa: morphological response to reduced illumination. Br Phycol J 11: 203–214
Cande WZ (1986) Reactivation of mitosis in vitro. TIBS 11: 447–449
Doree M, Picard A (1980) Release of Ca2+ from intracellular pools stops cytoplasmic streaming inTradescantia staminal hairs. Experientia 36: 1291–1292
Dostal R (1929) Untersuchungen über Protoplasmamobilisation beiCaulerpa prolifera. Jahrb Wiss Bot 71: 596–667
Hahne G, Hoffmann F (1984) The effect of laser microsurgery on cytoplasmic strands and cytoplasmic streaming in isolated protoplasts. Eur J Cell Biol 33: 175–179
Ishizaki Y, Mikawa T, Ebashi S, Yokota E, Hosoya H, Kuroda K (1988) Preparation of tubulin fromCaulerpa, a marine green alga, using casein as a protective agent against proteolytic degradation. J Biochem 104: 329–332
Janse JM (1890) Die Bewegungen des Protoplasmas vonCaulerpa prolifera. Pringsheim Jahrb Wiss Bot 21: 163–284
Kamiya N (1986) Cytoplasmic streaming in giant algal cells: a historical survey of experimental approaches. Bot Mag 99: 444–467
Koonce MP, Euteneuer U, McDonald KL, Menzel D, Schliwa M (1986) Cytoskeletal architecture and motility in a giant freshwater ameba,Reticulomyxa. Cell Motil Cytoskel 6: 521–533
Kreimer G, Surek B, Heimann K, Buchert M, Lukow L, Holtum JAM, Woodrow IE, Melkonian M, Latzko E (1987) Calcium metabolism in chloroplasts and protoplasts. In: Biggins J (ed) Progress in photosynthesis research, vol 3. Martinus Nijoff, Dordrecht, pp 4.345–4.357
Kuroda K, Manabe E (1984) Microtubules associated cytoplasmic streaming inCaulerpa. Proc Japan Acad [B] 59: 131–134
Küster E (1956) Die Pflanzenzelle. Bewegungserscheinungen des lebendigen Cytoplasmas. VEB G Fischer, Jena, pp 62–73
La Claire II, JW (1982) Wound-healing motility in the green algaErnodesmis: calcium ions and metabolic energy are required. Planta 156: 466–474
McKerracher LJ, Heath IB (1986 a) Fungal nuclear behavior analysed by ultraviolet microbeam irradiation. Cell Motil Cytoskel 6: 35–47
— — (1986 b) Polarized cytoplasmic movement and inhibition of saltations induced by calcium-mediated effects of microbeams in fungal hyphae. Cell Motil Cytoskel 6: 136–145
Manabe E, Kuroda K (1984) Ultrastructural basis of the microtubule-associated cytoplasmic streaming inCaulerpa. Proc Japan Acad [B] 60: 118–121
Menzel D (1985) Fine structure study on the association of the caulerpalean plastid with microtubule bundles in the siphonalean green algaChlorodesmis fastigiata Ducker (Udoteaceae). Protoplasma 125: 103–110
— (1986) Visualization of cytoskeletal changes through the life cycle inAcetabularia. Protoplasma 134: 30–42
— (1987) The cytoskeleton of the giant coenocytic green algaCaulerpa visualized by immunocytochemistry. Protoplasma 139: 71–76
— (1988) How do giant plant cells cope with injury. The wound response in siphonous green algae. Protoplasma 144: 73–91
- Elsner-Menzel C (1989) Induction of actin-based cytoplasmic contraction in the siphonous green algaAcetabularia (Chlorophyceae) by locally restricted calcium influx. Botanica Acta 102, in press
Mita T, Shibaoka H (1984) Gibberellin stabilizes microtubules in onion leaf sheath cells. Protoplasma 119: 100–109
Moore AL, Proudlove MO, Ackerman KEO (1986) The role of intracellular organelles in the regulation of cytosolic calcium levels. In: Trewavas AJ (ed) Molecular and cellular aspects of calcium in plant development. Plenum Press, New York London
Pemrick S, Weber A (1976) Mechanism of inhibition of relaxation by N-ethylmaleimide treatment of myosin. Biochemistry 15: 5193–5198
Sabnis DD, Jacobs WP (1967) Cytoplasmic streaming and microtubules in the coenocytic marine algaCaulerpa prolifera. J Cell Sci 2: 465–472
Sheetz MP, Spudich JA (1983) Movement of myosin-coated fluorescent beads on actin cables in vitro. Nature 303: 31–35
Takagi S, Nagai R (1986) Intracellular Ca2+ concentration and cytoplasmic streaming inVallisneria mesophyll cells. Plant Cell Physiol 27: 953–959
Vale RD, Schnapp BJ, Reese TS, Sheetz MP (1985) Movement of organelles along filaments dissociated from the axoplasm of the squid giant axon. Cell 40: 449–454
Weidinger M, Ruppel HG (1985) Ca2+-requirement for a blue-light-induced chloroplast translocation inEremosphera viridis. Protoplasma 124: 184–187
Witztum A, Parthasarathy MV (1985) Role of actin in chloroplast clustering and banding in leaves ofEgeria, Elodea andHydrilla. Eur J Cell Biol 39: 21–26
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Menzel, D., Elsner-Menzel, C. Actin-based chloroplast rearrangements in the cortex of the giant coenocytic green algaCaulerpa . Protoplasma 150, 1–8 (1989). https://doi.org/10.1007/BF01352915
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DOI: https://doi.org/10.1007/BF01352915