Summary
The purpose of the present study was to determine if the type of dietary fat can modify the fatty acid composition and arachidonic acid levels in the alveolar bone phospholipids. Three groups of rats were fed nutritionally adequate semipurified diets containing different lipids: 10% corn oil (control, group 1, rich in n-6 fatty acids); 9% butter + 1% corn oil (experimental, group 11, rich in saturated fatty acids); and 9% ethyl ester concentrate of n-3 fatty acids + 1% corn oil (experimental, group 111, rich in n-3 fatty acids). After 10 weeks of feeding the various diets, rats were killed, maxillae and mandibles were dissected out, and the soft tissue was removed. Bone was frozen in liquid nitrogen and pulverized. Powdered bone was extracted for total lipids, and phospholipids were isolated by column chromatography. The fatty acid composition and arachidonic acid concentrations were determined in total phospholipids after the addition of an internal standard, octadecatetraenoic acid (18: 4n-3), and subsequent gas chromatography. The type of dietary lipids had a profound influence on the fatty acid composition of bone lipids. Arachidonic acid concentrations were significantly lower in total phospholipids of mandibles and maxillae of rats fed the experimental diets than in those fed the control diet. Because arachidonic acid is a precursor of prostaglandin E2 and leukotriene C4, a significant reduction in its concentration may result in reduced levels of these eicosanoids in the alveolar bone.
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References
Das SK, Scott MT, Adhikary PK (1975) Effect of the nature and amount of dietary energy on lipid composition of rat bone marrow. Lipids 10:584–590
Watkins BA, Whitehead CC, Duff SR (1991) Hydrogenated oil decreases tissue concentrations of n-6 polyunsaturated fatty acids and many contribute to dyschondroplasia in broilers. Br Poult Sci 32:1109–1119
American Institute of Nutrition (1977) Report on the AIN Ad Hoc Committee on Standards for Nutritional Studies. J Nutr 107:1340–1348
American Institute of Nutrition (1980) Second Report of the Ad Hoc Committee on Standards for Nutritional Studies. J Nutr 110:1726
Mularchuck P, Boskey A (1990) Lipids in bone: optimal conditions for tissue storage prior to lipid analyses. Calcif Tissue Int 46:57–59
Folch J, Lees M, Sloane Stanley GH (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226:497–509
Morrison WR, Smith LM (1964) Preparation of fatty acid methyl esters and dimethylacetals from lipids with boron fluoridemethanol. J Lipid Res 5:600–608
Alam SQ, Bergens BM, Alam BS (1991) Arachidonic acid, prostaglandin E2 and leukotriene C4 levels in gingiva and salivary glands of rats fed diets containing n-3 fatty acids. Lipids 26:895–900
Cook HW (1991) Fatty acid desaturation and chain elongation in eucaryotes. In: Vance DE, Vance J (eds) Biochemistry of lipids, Lipoproteins and Membranes. Elsevier, Amsterdam, p 160
Kokkinos PP, Shaye R, Alam BS, Alam SQ (1993) Dietary lipids, prostaglandin E2 levels and tooth movement in alveolar bone of rats. Calcif Tissue Int 53:333–337
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Alam, S.Q., Kokkinos, P.P. & Alam, B.S. Fatty acid composition and arachidonic acid concentrations in alveolar bone of rats fed diets with different lipids. Calcif Tissue Int 53, 330–332 (1993). https://doi.org/10.1007/BF01351838
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DOI: https://doi.org/10.1007/BF01351838