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Selenium deficiency in Crustacea

An ultrastructural approach to antennal damage inDaphnia magna Straus

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Summary

The effect of selenium deprivation onDaphnia magna was examined under controlled rearing conditions in a synthetic culture medium. After three generations, fertility was significantly reduced in deprived (Se−) animals. Growth and mortality of parent daphnids and development of parthenogenetic eggs were not affected during this period. In the fourth generation Se− daphnids rejected parts of their second antennae. At the ultrastructural level antennal muscle tissue was severely affected. Animals deprived of selenium had mitochondria and sarcoplasmic reticulum with myelin-like alterations. Giant lysosomes were present and complete lysis of muscle fibrils was observed in antennal muscle cells. These alterations are characteristic features of peroxidic damage in tissues. This interpretation is consistent with the function of selenium as a constituent of the enzyme glutathione peroxidase which protects cells from peroxidation. Selenium should be included in synthetic culture media for daphnids.

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Abbreviations

GSH-Px:

selenium dependent glutathione peroxidase

Se+/Se− :

selenium supplemented (control)/selenium deprived animals

SOD:

Superoxide dismutase

SR:

sarcoplasmic reticulum

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  1. Zoology Department I (Morphology/Ecology), University of Heidelberg, Heidelberg

    B. -P. Elendt

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Elendt, B.P. Selenium deficiency in Crustacea. Protoplasma 154, 25–33 (1990). https://doi.org/10.1007/BF01349532

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  • DOI: https://doi.org/10.1007/BF01349532

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