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Radiation effects on cell membranes

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Summary

The recent developments in the field of membrane biology of eukaryotic cells result in revival of relevant radiobiological studies. The spatial relations and chemical nature of membrane components provide rather sensitive targets. Experimental data are presented concerning the effects of relatively low doses of X-irradiation and low concentration of tritiated water (HTO) on variou receptor function - concanavalin A, cationized ferritin, poliovirus - of plasma membranes of animal and human cells which point to early and temporary disturbances of the composite structures and functions of membranes. References are given to the multifold roles of radiation-induced membrane phenomena on the development and regeneration of radiation injuries. Invited plenary lecture presented at 16th Annual Meeting of the European Society for Radiation Biology, Krakow, Poland, 7–10 September 1981

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References

  1. Alper T (1979) Cellular radiobiology. Cambridge Univ. Press, Cambridge

    Google Scholar 

  2. Bartosz G, Leyko W, Kedziora J, Jeske J (1980) Superoxide dismutase and radiation-induced haemolysis: no benefit of its increased content in red cells. Int J Biol 38: 187–192

    Google Scholar 

  3. Brauer B, Booz J (1981) Primary biological effects of ionizing radiation and their consequences: a theoretical approach Abstr., 16th Ann. Meeting of ESRB, Krakow, Poland

    Google Scholar 

  4. Bell GI (1978) Models for the specific adhesion of cells to cells Science 200: 618–627

    Google Scholar 

  5. Boldyrev AA (1981) Role of lipids in the functioning of Na, K-ATPase. Stud Biophys Berlin 84: 153–160

    Google Scholar 

  6. DePierre JW, Dallner G (1975) Structural aspects of the membrane of the EPR. Biochim Biophys Acta 415: 411–472

    Google Scholar 

  7. Feinendegen LE, Mühlensiepen H, Porschen W, Booz J (1981) Acute non-stochastic effect of very low dose wholebody exposure, a thymidine equivalent serum factor Int J Radiat Biol (in press)

  8. Ferber E (1978) Die Rolle der Membran-Phospholipide bei der Stimulation von Lymphocyten. Blut 36: 255–261

    Google Scholar 

  9. Ferber E, Kröner E, Schmidt B, Fischer H, Peskar BA, Anders C (1980) Dynamics of membrane fatty acids during lymphocyte sitmulation by mitogens. In “Membrane fluidity” ed. by M. Kates and A. Kuksis, The Humana Press Clifton N.J., pp 239–263.

  10. Floyd RA (1980) Free radical events in carcinogenesis. Radiat Res 83: 442

    Google Scholar 

  11. Fridovich I (1972) Superoxide radical and superoxide dismutase. Abst Chem Res 5: 321

    Google Scholar 

  12. George KC, Srinivasan VT, Singh BB (1980) Cytotoxic effect of chlorpromazine and its interaction with radiation on a mouse fibrosarcoma. Int J Radiat Biol 38: 661–666

    Google Scholar 

  13. Goetzl EJ (1980) Vitamin E modulates the lipoxygenation of arachidonic acid in leukocytes. Nature (London) 288: 183–185

    Google Scholar 

  14. Grzelinska E, Bartosz G, Gwozdzinski K, Leyko W (1979) A spin-label study of the effect of gamma radiation on erythrocyte membrane. Influence of lipid peroxidation on membrane structure. Int J Radiat Biol 36: 325–344

    Google Scholar 

  15. Hirata F, Axelrod J (1980) Phospholipid methylation and biological signal transmission. Science 209: 1082–1090

    Google Scholar 

  16. Hrycay EG, O'Brien PJ (1971) Cytochrome P-450 as a microsomal peroxide utilizing a lipid peroxide substrate. Arch Biochem Biophys 147: 14–27

    Google Scholar 

  17. Ivanov IF (1976) On the role played by tocopherol (vitamin E) in the additional harmful action produced by postirradiation hyperoxia Radiobiologiya 16: 773–776

    Google Scholar 

  18. Kagan VE, Prilipko LL, Savov VM, Pisarev VA, Eluashvili IE, Kozlov Yu P (1979) Participation of oxygen activated species in enzymatic lipid peroxidation in biomembranes Biochimiya 44: 482–489

    Google Scholar 

  19. Kimura A, Fujimura K, Kuramoto A (1979) Superoxide dismutase of human platelets. Its activity in myeloproliferative disorders and the effect of fluoride, aggregating agents and irradiation. Ketsucki Myakkan 10: 449–452 (ref. INIS 548599)

    Google Scholar 

  20. Konings AWT, Drijver EB (1979) Radiation effects on membranes. I. vitamin E deficiency and lipid peroxidation. Radiat res 80: 494–501

    Google Scholar 

  21. Konings AWT (1979) Dose-rate effects in biomembranes J Radiat Res 20: 259–263

    Google Scholar 

  22. Konings AWT, Oosterloo SK (1980) Radiation effects on membranes. II. Comparison of the effects of x-irradiation and ozone exposure with respect to the relation of antioxidant concentration and the capacity for lipid peroxidation Radiat Res 81: 200–207

    Google Scholar 

  23. Konings AWT (1981) Radiation-induced efflux of potassium ions and haemoglobin in bovine erythrocytes at low doses and low dose-rates Int J Radiat Biol 40: 441–444

    Google Scholar 

  24. Konings AWT (1981) Dose-rate effects on lymphocyte survival J Radiat Res 22: 282–285

    Google Scholar 

  25. Konings AWT (1981) Membrane involvement in hyperthermic cell damage and heat potentiated radiation-induced cell killing Strahlentherapie 157: 613–624

    Google Scholar 

  26. Konings AWT (1981) Dose-rate effects on membrane functions Abstr., 16th Ann. Meeting of ESRB, Krakow, Poland

  27. Kozhokaru AF, Zaslavskii Yu A, Akoev IG, Alekseeva LV (1980) The stabilizing effect of antioxidants on membranes affected by ionizing radiation Radiobiologiya 20: 902–905

    Google Scholar 

  28. Köteles GJ, Kubasova T, Varga L (1976) H-3-concanavalin A binding of x-irradiated human fibroblasts. Nature (London) 259: 507–508

    Google Scholar 

  29. Köteles GJ (1979) New aspects of cell membrane radiobiology and their impact on radiation protection. At En Rev 17: 3–30

    Google Scholar 

  30. Köteles GJ, Kubasova T, Horváth L, Somosy Z (1981) Functional receptor alterations in radiation perturbed plasma membranes. 16th Ann. Meeting ESRB, Krakow, Poland

  31. Krasichkova ZI, Strazhevskaya NB (1981) Role of lipid component in the mechanism of postirradiation degradation and repair of supramolecular DNA of the rat thymus and liver. Radiobiologiya 21: 172–179

    Google Scholar 

  32. Kratochvilova V, Krizala J, Ledvina M (1981) Activity of superoxide dismutase in erythrocytes of irradiated rats. Studia Biophys Berlin 82: 121–126

    Google Scholar 

  33. Krizala J, Ledina M (1980) The activity of superoxide dismutase in the liver and bone marrow of gamma-irradiated rats. Int J Radiat Biol 37: 459–462

    Google Scholar 

  34. Kubasova T, Varga L, Köteles GJ (1975) Effect of irradiation on H-3-glucosamine uptake by human embryo fibroblasts. Int J Radiat Biol 27: 325–335

    Google Scholar 

  35. Kubasova T, Varga L, Köteles GJ (1976) Studies on the radiosensitivity of the function of the Golgi complex. Int J Radiat Biol 29: 533–540

    Google Scholar 

  36. Kubasova T, Csáky L, Köteles GJ, Varga L, Sztanyik LB (1977) Early detection of radiation-induced cell membrane alterations by lectin-binding. Proc. 4th Int. Congr. IRPA, Paris

    Google Scholar 

  37. Kubasova T, Köteles GJ (1977) On the radiation biological relation between the metabolism of Golgi complex and plasma membrane. Izotoptechnika (Budapest) 20: 450–456

    Google Scholar 

  38. Kubasova T, Varga LP, Köteles GJ (1981) Surface alterations of mammalian cells upon ionizing radiation as detected by a lectin-binding technique. I. Binding of concanavalin A by blood cells of x-irradiated mice. Int J Radiat Biol 40: 175–186

    Google Scholar 

  39. Kubasova T, Köteles GJ, Varga LP (1981) Surface alterations of mammalian cells upon ionizing radiation as detected by lectin-binding technique. II. Binding of concanavalin A by human blood cells x-irradiated in vitro. Int J Radiat Biol 40: 187–194

    Google Scholar 

  40. Lipecka K, Lipinski St, Kanski M (1978) The influence of gamma irradiation and cysteamine on superoxide dismutase activity in rabbit liver Studia. Biophys Berlin 68: 25–30

    Google Scholar 

  41. Lipinski St, Lipecka K, Donec Ya, Kanski M (1976) Action of ionizing radiation of the activity of superoxidedismutase as measured by the chemiluminescent method. Radiobiologiya 16: 665–670

    Google Scholar 

  42. Matkovich B (1977) Superoxide and superoxide dismutases Michelson AM, Acad. Press, London, pp 501–515

    Google Scholar 

  43. Miller GG, Raleigh JA (1981) Low dose rate studies and the mechanism of interphase cell death. Radiat Res 87: 401

    Google Scholar 

  44. Mirakhmedov AK, Muradillaev A, Khamidov D kh (1981) The effect of ionizing radiation on membrane-bound enzymes from liver mitochondria. 16th Ann. Meeting of ESRB, Krakow, Poland

  45. Mooibroek J, Konings AWT (1980) Structural dependency of membrane lipids with regard to radiosensitivity. Radiat Environ Biophys 17: 301

    Google Scholar 

  46. Myers DK (1970) Some aspects of radiation effects on cell membranes. Adv Biol Med Phys 13: 219–233

    Google Scholar 

  47. Nolan WT, Thompson JE, Lepock JR, Kruuv J (1981) Effect of membrane lipid perturbers on the temperature dependence of repair of sublethal and potentially lethal radiation damage. Int J Radiat Biol 39: 195–205

    Google Scholar 

  48. Packer JE, Slater TF, Willson RL (1979) Direct observation of a free radical interaction between vitamin E and vitamin C. Nature (London) 278: 737–738

    Google Scholar 

  49. Patrick G (1977) The effects of radiation on cell membranes. In: Jamieson GA, Robinson DM (eds) Mammalian cell membranes. Vol 5, Butterworths, London, p 72

    Google Scholar 

  50. Petkau A, Chelack WS (1976) Radioprotective effect of superoxide dismutase on model phospholipid membranes. Biochem Biophys Acta 433: 445–456

    Google Scholar 

  51. Petkau A (1978) Radiation protection by superoxide dismutase. Photochem Photobiol 28: 765–774

    Google Scholar 

  52. Petrone WF, English DK, Wong K, McCord JM (1980) Free radicals and inflammation: superoxide-dependent activation of a neutrophil chemotactic factor in plasma. Proc Natl Acad Sci USA 77: 1159–1163

    Google Scholar 

  53. Purohit SC, Bisby RH, Cundall RB (1980) Structural modification of human erythrocyte membranes following gamma-irradiation. Int J Radiat Biol 38: 147–158

    Google Scholar 

  54. Purohit SC, Bisby RH, Cundall RB (1980) Chemical damage in gamma-irradiated human eryhtrocyte membranes. Int J Radiat Biol 38: 159–166

    Google Scholar 

  55. Raeva NF (1980) Role of malondialdehyde and similar compounds in the formation of cross-links in gamma-irradiated DNA. Radiobiologiya 20: 664–670

    Google Scholar 

  56. Rigas DA, Eginitis-Rigas C, Head C (1979) Biphasic toxicity of diethyldithiocarbamate, a metal chelator, to T lymphocytes and polymorphonuclear granulocytes: reversal by zinc and copper. Biochem Biophys Res Commun 88: 373–379

    Google Scholar 

  57. Rigas DA, Eginitis-Rigas C, Bigley RH, Stankova L, Head C (1980) Biphasic radiosensitization of human lymphocytes by diethyldithiocarbamate: possible involvement of superoxide dismutase. Int J Radiat Biol 38: 257–266

    Google Scholar 

  58. Sato C, Kojima K (1974) Change in electrophoretic mobility of cultured cells after x-irradiation and their modification by SH-blocking agents and hemagglutinin. Radiat Res 60: 506–515

    Google Scholar 

  59. Sato C, Nishizawa K, Kojima K (1979) Calcium-dependent process in reduction of cell surface charge after x-irradiation. Int J Radiat Biol 35: 221–228

    Google Scholar 

  60. Schanne FAX, Kane AB, Young EE, Farber JL (1979) Calcium dependence of toxic cell death: a final common pathway. Science 206: 700–702

    Google Scholar 

  61. Schorn H, Bertsche U, Nüsse M, Bryant PE (1981) Cellular response to membrane-specific drugs and ionizing radiation. Radiat Environ Biophys 19: 301

    Google Scholar 

  62. Schweitzer K, Benkö Gy (1980) Personal communication

  63. Singer SJ, Nicolson GL (1972) The fluid mosaic model of the structure of cell membranes. Science 175: 720–731

    Google Scholar 

  64. Somosy Z, Kubasova T, Köteles GJ (1981) The effects of x-irradiations on the surface morphology of human embryo fibroblasts. 16th Ann. Meeting of ESRB, Krakow, Poland

  65. Somosy Z, Kubasova T, Köteles GJ (1982) Scanning electronmicroscopic appearance of x-irradiated human fibroblasts J Radiat Biol (in press)

  66. Stone D, Lin PS, Kwock L (1978) Radiosensitization of human erythrocytes bxy diethyldithiocarbamate. Int J Radiat Biol 33: 393–396

    Google Scholar 

  67. Strazhevskaya NB, Struchkov VA, Strelkov RB, Ronin M Ya, Chizhov A Ya (1977) Modification of radiosensitivity of DNA-membrane complexes in mammalian cells by means of protectors Proc. 4th Int. Congr. IRPA, Paris

    Google Scholar 

  68. Strazhevskaya NB, Struchkov VA (1977) Organization of supramolecular DNA complexes of the eucaryote chromatin and their role in the radiation effect. Radiobiologiya 17: 163–177

    Google Scholar 

  69. Strazhevskaya NB, Krasichkova ZI, Kruglova NL (1980) Radiation damage and repair of DNA-membrane complex in mammalian cells. Studia Biophys Berlin 76: 205–210

    Google Scholar 

  70. Symonyan MA, Nalbandyan RM (1979) The effect of x-rays on properties of superoxide dismutase. Biochem Biophys Res Commun 90: 1207–1213

    Google Scholar 

  71. Szamel M, Somogyi J, Resch K (1982) Role of plasmamembrane in the regulation of lymphocyte stimulation. Biol Rep Hung Acad Sci (in press)

  72. Szamel M, Resch K (1981) Modulation of enzyme activities in isolated lymphocyte plasma membranes by enzymatic modification of phospholipid fatty acids. J Biol Chem 256: 11618–11623

    Google Scholar 

  73. Szekely JG, Copps TP, Morash BD (1980) Radiation-induced invagination of the nuclear envelope. Radiat Res 83: 621–632

    Google Scholar 

  74. Szumiel I (1981) Intrinsic radiosensitivity of proliferating mammalian cells. Adv Radiat Biol 9: 281–321

    Google Scholar 

  75. Wallach DHF (1974) Radiation effects on Biomembranes. Biomembranes 5: 213–249

    Google Scholar 

  76. Westman G, Marklund SL (1980) Diethyldithiocarbamate, a superoxide dismutase inhibitor, decreases the radioresistance of chinese hamster cells. Radiat Res 83: 303–311

    Google Scholar 

  77. Wojtczak L, Nalecz MJ (1979) Surface charge of biological membranes as a possible regulator of membrane-bound enzymes. Eur J Biochem 94: 99–107

    Google Scholar 

  78. Yau TM (1979) Procaine-mediated modification of membranes and of the response to x-irradiation and hyperthermia in mammalian cells. Radiat Res 80: 523–541

    Google Scholar 

  79. Yau TM, Mencl J (1981) A study of the peroxidation of fatty acid micelles promoted by ionizing radiation, hydrogen peroxide and ascorbate. Int J Radiat Biol 40: 47–61

    Google Scholar 

  80. Yonei S, Furui H (1981) Lethal and mutagenic effects of malondialdehyde, a decomposition product of peroxidized lipids, on E. coli with different DNA-repair capacities Mutat Res 88: 23–32

    Google Scholar 

  81. Yukawa O, Nakazawa T (1980) Radiation-induced lipid peroxidation and membrane-bound enzymes in liver microsomes. Int J Radiat Biol 37: 621–631

    Google Scholar 

  82. Zamboglou N, Porschen W, Muhlensiepen H, Booz J, Feinendegen LE (1981) Low dose effect of ionizing radiation on incorporation of iododeoxyuridine into bone marrow cells. Int J Radiat Biol 39: 83–93

    Google Scholar 

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  1. “Frederic Joliot-Curie” National Research Institute for Radiobiology and Radiohygiene, H-1775, Budapest, Hungary

    G. J. Köteles

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Köteles, G.J. Radiation effects on cell membranes. Radiat Environ Biophys 21, 1–18 (1982). https://doi.org/10.1007/BF01338751

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