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An analysis of the distribution and dose response of chromosome aberrations in human lymphocytes after in vitro exposure to137Cesium gamma radiation

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Summary

The chromosome aberration yield for human lymphocytes exposed in vitro to various doses of137Cesium has been studied. Dicentric, total acentric, and excess acentric data were seen to follow a Possion distribution. Calculated total hits demonstrated over-dispersion which could possibly be accounted for by a greater occurrence of single-hit phenomena being repaired than two-hit exchange processes. The resulting distribution generally contained an under-representation of cells with odd numbers of hits and an over-representation of zero- and even-hit classes as compared with Poisson predicted values. The relationship between dicentric yield and dose received in rads was fitted to the linear-quadratic formulaY =αD +βD2 for dicentrics, yielding values of (20.1 ± 3.8) × 10−4 (aberrations/cell)/rad and (1.89 ± 0.75) × 10−6 (aberrations/cell)/rad2 forα andβ respectively. A plot of percent ‘normal’ cells versus the dose in rads resembled cell survival curves and was fitted to the relationP(D) = 100 e−Y whereY =αD +βD2 withα = (23 ± 11) × 10−4 rad−1 andβ = (8.3 ± 2.5) × 10−6 rad−2. A possible use of scoring ‘normal’ cells for purposes of biological dosimetry is presented.

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Author notes

  1. Norman A. Doggett

    Present address: Department of Pathology, School of Medicine, University of North Carolina, 27514, Chapel Hill, North Carolina

Authors and Affiliations

  1. Department of Genetics, North Carolina State University, 27650, Raleigh, NC, USA

    Norman A. Doggett & Wendell H. McKenzie

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  1. Norman A. Doggett
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  2. Wendell H. McKenzie
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Additional information

This study was supported in part by U.S. Environmental Protection Agency Grant R-805739-03

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Doggett, N.A., McKenzie, W.H. An analysis of the distribution and dose response of chromosome aberrations in human lymphocytes after in vitro exposure to137Cesium gamma radiation. Radiat Environ Biophys 22, 33–51 (1983). https://doi.org/10.1007/BF01323759

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  • DOI: https://doi.org/10.1007/BF01323759

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