Pollen development in orchids 1. Cytoskeleton and the control of division plane in irregular patterns of cytokinesis

Summary

The cytokinetic apparatus in microsporogenesis lacks a preprophase band of microtubules and the selection of cytokinetic planes is dependent upon disposition of nuclei which define cytoplasmic domains via post-meiotic radial systems of microtubules. Meiotic cytokinesis was investigated in hybrid moth orchids (Phalaenopsis) exhibiting irregular patterns of cytokinesis. In these polliniate orchids, spindle orientation is imprecise, and the tetrad nuclei (therefore the microspores) may be in rhomboidal, tetrahedral or linear arrangement. The hybrid “Sabine Queen” (section Phalaenopsis) regularly undergoes simultaneous cytokinesis, as is common in orchids. The hybrid “Vista Rainbow” (section Amboinenses) produces either a complete dyad wall, a partial wall, or no wall after first nuclear division. In all cases, a first division phragmoplast is initiated in the interzonal region and expands centrifugally into the peripheral cytoplasm. Fluorescence microscopy shows that the phragmoplast consists of fusiform bundles of microtubules and Factin bisected by a non-fluorescent zone. If a cell plate fails to form, a band of organelles polarized in the equatorial region effectively divides the cell into two domains. The organelles disperse when a dyad wall is complete, but tend to remain polarized around an incomplete wall. In four-nucleate coenocytes, the usual interzonal microtubules between sister nuclei (primary) form slightly in advance of secondary arrays between non-sister nuclei. Phragmoplasts are initiated in sites defined by the post-meiotic microtubule arrays.