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Microtubule organization during development of stomatal complexes inLolium rigidum

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Summary

Microtubule (MT) arrays in stomatal complexes ofLolium have been studied using cryosectioning and immunofluorescence microscopy. This in situ analysis reveals that the arrangement of MTs in pairs of guard cells (GCs) or subsidiary cells (SCs) within a complex is very similar, indicating that MT deployment is closely coordinated during development. In premitotic guard mother cells (GMCs), MTs of the transverse interphase MT band (IMB) are reorganized into a longitudinal array via a transitory array in which the MTs appear to radiate from the cell edges towards the centre of the walls. Following the longitudinal division of GMCs, cortical MTs are reinstated in the GCs at the edge of the periclinal and ventral walls. The MTs become organized into arrays which radiate across the periclinal walls, initially from along the length of the ventral wall and later only from the pore site. As the GCs elongate, the organization of MTs and the patterns of wall expansion differ on the internal and external periclinal walls. A final reorientation of MTs from transverse to longitudinal is associated with the elongation and constriction of GCs to produce mature complexes. During cytokinesis in the subsidiary mother cells (SMCs), MTs appear around the reforming nucleus in the daughter epidermal cells but appear in the cortex of the SC once division is complete. Our results are thus consistent with the idea that interphase MTs are nucleated in the cell cortex in all cells of the stomatal complex but not in adjacent epidermal cells.

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Abbreviations

GMC:

guard mother cell

GC:

guard cell

IMB:

interphase microtubule band

MT:

microtubule

PPB:

preprophase band

SMC:

subsidiary mother cell

SC:

subsidiary cell

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Authors and Affiliations

  1. Plant Cell Biology Group, Research School of Biological Sciences, The Australian National University, Canberra

    A. L. Cleary & A. R. Hardham

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  1. A. L. Cleary
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  2. A. R. Hardham
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Cleary, A.L., Hardham, A.R. Microtubule organization during development of stomatal complexes inLolium rigidum . Protoplasma 149, 67–81 (1989). https://doi.org/10.1007/BF01322979

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  • DOI: https://doi.org/10.1007/BF01322979

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