Skip to main content
SpringerLink
Log in

Comparative nucleotide and deduced amino acid sequence analysis of VP7 gene of the NCDV Cody (I-801) strain of group A bovine rotavirus

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

The prevalent G serotypes of group A bovine rotavirus (BRV) reported are G6, G10, and less commonly, G8. Neonatal Calf Diarrhea Virus (NCDV), Lincoln and Cody strains were first isolated from diarrheic calves in Nebraska. The NCDV Lincoln strain is the currently used U.S. vaccine strain and has a G6 serotype. In this study, the complete nucleotide sequence of the VP7 gene of NCDV Cody (I-801 strain) was determined using the primer extension method. The VP7 gene nucleotide sequence homologies between Cody I-801 and established G8 rotaviruses, A5 (Thailand BRV), 678 (UK BRV), B37 (human RV) and 69M (human RV) were 84.7%, 86.4%, 84.7% and 85.9%, respectively. The deduced VP7 amino acid sequence of Cody I-801 was similar to that of A5, 678, B37 and 69M (93.6%, 95.7%, 92.6% and 95.1%, respectively). The VP7 gene nucleic acid sequence homologies between NCDV Cody (I-801) and NCDV Lincoln or B223 (G10) was 76.2% and the deduced VP7 amino acid sequence homologies between Cody I-801 and NCDV Lincoln or B223 were 82.5% and 81.3%, respectively. Thus, our sequence data suggests that the VP7 gene of Cody I-801 strain of BRV is genetically most similar to G8 rotaviruses and unrelated to the NCDV Lincoln G6 rotavirus strain.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Albert MJ, Unicomb LE, Bishop RF (1987) Cultivation and characterization of human rotaviruses with super-short RNA patterns. J Clin Microbiol 25: 183–185

    Google Scholar 

  2. Blackhall J, Bellinzoni R, Mattion N, Estes MK, LaTorre JL, Magnusson G (1992) A bovine rotavirus serotype 1: serological characterization of the virus and nucleotide sequence determination of the structural glycoprotein VP7 gene. Virology 189: 833–837

    Google Scholar 

  3. Dyall-Smith ML, Lazdins I, Tregar GW, Holmes IH (1986) Location of the major antigenic sites involved in rotavirus serotype-specific neutralization. Proc Natl Acad Sci USA 83: 3465–3468

    Google Scholar 

  4. Gerna G, Steele AD, Hoshino Y, Sereno M, Garcia D, Sarasini A, Flores J (1994) A comparison of the VP7 gene sequences of human and bovine rotaviruses. J Gen Virol 75: 1781–1784

    Google Scholar 

  5. Green KY, Midthun K, Gorziglia M, Hoshino Y, Kapikian AZ, Chanock RM, Flores J (1987) Comparison of the amino acid sequences of the major neutralization protein of four human rotavirus serotypes. Virology 161: 153–159

    Google Scholar 

  6. Gubler U, Hoffman BJ (1983) A simple and very effective method for generating cDNA libraries. Gene 25: 263–269

    Google Scholar 

  7. Hussein HA, Parwani AV, Rosen BI, Lucchelli A, Saif LJ (1993) Detection of rotavirus serotypes G1, G2, G3 and G11 in faces of diarrheic calves by using polymerase chain reaction derived cDNA probes. J Clin Microbiol 31: 2491–2496

    Google Scholar 

  8. Li B, Clark HF, Gouvea V (1994) Amino acid sequence similarity of the VP7 protein of human rotavirus HCR3 to that of canine and feline rotaviruses. J Gen Virol 75: 215–219

    Google Scholar 

  9. Matsuno S, Hasegawa A, Mukoyama A, Inouye S (1985) A candidate for a new serotype of human rotavirus. J Virol 54: 623–624

    Google Scholar 

  10. Mebus CA, Underdahl NR, Twiehaus MJ (1969) Calf diarrhea (scours): reproduced with a virus from a field outbreak. Univ Nebraska Res Bull 233: 1–6

    Google Scholar 

  11. Nagesha HS, Huang J, Holmes IH (1992) A variant serotype G3 rotavirus isolated from an unusually severe outbreak of diarrhoea in piglets. J Med Virol 38: 79–85

    Google Scholar 

  12. Ohshima A, Takagi T, Nakagomi T, Matsuno S, Nakagomi O (1990) Molecular characterization by RNA-RNA hybridization of a serotype 8 human rotavirus with “super-short” RNA electropherotype. J Med Virol 30: 107–112

    Google Scholar 

  13. Saif LJ, Rosen BI, Parwani AV (1994) Animal rotaviruses. In: Kapikian AZ (ed) Viral infections of the gastrointestinal tract, 2nd edn. Marcel Dekker, New York, pp 289–314

    Google Scholar 

  14. Shen S, Burke B, Desselberger U (1993) Nucleotide sequences of the VP4 and VP7 genes of a chinese lamb rotavirus: evidence for a new P type in a G10 type virus. Virology 197–500

  15. Snodgrass DR, Fitzgerald T, Cambell I, Scott FMM, Browning GF, Miller DL, Herring AJ, Greenberg HB (1990) Rotavirus serotypes 6 and 10 predominate in cattle. J Clin Microbiol 28: 504–507

    Google Scholar 

  16. Taniguchi K, Urasawa T, Pongsuwanna Y, Choonthanom M, Jayavasu C, Urasawa S, (1991) Molecular and antigenic analysis of serotype 8 and 10 bovine rotaviruses in Thailand. J Gen Virol 72: 2929–2937

    Google Scholar 

  17. Webster RG, Laver WG, Air GM, Schild GC (1982) Molecular mechanisms of variation in influenza viruses. Nature 296: 115–121

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Food Animal Health Research Program, Department of Veterinary Preventive Medicine, Ohio Agricultural Research and Development Center, The Ohio State University, 444691, Wooster, Ohio, USA

    K. O. Chang, A. V. Parwani &  L. J. Saif

Authors
  1. K. O. Chang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. V. Parwani
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. L. J. Saif
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chang, K.O., Parwani, A.V. & Saif, L.J. Comparative nucleotide and deduced amino acid sequence analysis of VP7 gene of the NCDV Cody (I-801) strain of group A bovine rotavirus. Archives of Virology 140, 1279–1283 (1995). https://doi.org/10.1007/BF01322753

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01322753

Keywords

Search

Navigation