Summary
If rabbits were given total body irradiation and infected with vaccinia virus (strain Elstree) a severe disease developed with a viraemia lasting up to 12 days. The clearance of the virus from the peripheral blood was severely impaired by x-ray doses above 800 R. The attenuated vaccinia virus strain MVA did not turn virulent, if it was injected to irradiated rabbits. With caution it can be assumed that live vaccines, containing attenuated viruses, may be given to immunosuppressed persons. Rats are not susceptible to ectromelia-virus (mouse-poxvirus); overt clinical symptoms, however, with a mortality of 30 per cent developed in irradiated rats. This proofs that specific poxviruses can be transferred to another species. As the experimental conditions are unnatural, this may occur only rarely in immunosuppressed persons. After intracerebral infection of Balb-C-mice with low doses of vaccinia virus two types of infection were seen: 1. a severe cytocidal infection of leptomeninges, chorioid plexus and vessels; 2. a noncytocidal, latent infection of glial cells and neurons. Several animals developed a picture resembling experimental allergic encephalomyelitis. It seems that irradiation altered the antigenic conditions of the cytoplasmic membranes in non-cytocidally infected cells. The model might explain some processes in the pathogenesis of demylinating diseases.
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References
Adams, R. D.: A comparison of the morphology of the human demyelinating disease and experimental allergic encephalomyelitis. In:Kies, M. W., Alford, E. C. (eds.), Allergic encephalomyelitis, 183–209. Springfield, Ill.: Ch. C Thomas 1959.
Anderson, R. E., Warner, N. L.: Ionizing radiation and immune response. Adv. Immunol.24, 216–320 (1976).
Bandlow, G., Fischer, W., Thompssen, R.: Gesteigerte Immunogenität von Plasmamembranen vacciniainfizierter BHK-Zellen. Arch. ges. Virusforsch.38, 63–71 (1973).
Blinzinger, K., Hochstein-Mintzel, V., Anzill, A. P.: Experimental vaccinia virus meningoencephalitis in adult albino mice. Acta neuropath. (Berl.)40, 193 to 205 (1977).
Bøyum, A.: Separation of leucocytes from blood and bone-marrow. Scand. J. clin. Lab. Invest.21, suppl. 97, 1–12 (1968).
Burnet, F. M., Lush, D.: Inapperent (subclinical) infection of the rat with the virus of infectious ectromelia of mice. J. Path. Bact.42, 469–480 (1936).
Downie, A. W., Kempe, C. H.: Poxviruses. In:Lenette, E. H., Schmidt, N. J. (eds.), Diagnostic procedures for viral and rickettsial infections, 4th ed., 314–316. New York: American Health Association 1969.
Dupuy, J. M., Levey-Leblond, E., Le Prevost, C.: Immunopathology of mouse hepatitis virus type 3 infection. J. Immunol.114, 226–230 (1975).
Ginsberg, A. M., Johnson, K. P.: Vaccinia virus meningitis in mice after intracerebral inoculation. Infect. Immun.13, 1221–1227 (1976).
Harter, D. H., Choppon, P. W.: Possible mechanisms in the pathogenesis of “postinfectious” incephalomyelitis. In:Rowland, L. P. (ed.), Immunological disorders of the nervous system, 342–357. Baltimore: The Williams and Wilkins Co. 1971.
Hirsch, M. S., Proffitt, M. R.: Autoimmunity in viral infections. In:Notkins, A. L. (ed.), Viral immunology and immunopathology, 419–434. New York: Academic Press 1975.
Hochstein-Mintzel, V.: Infektionsdosis, Inkubationszeit sowie Morbiditätsrate bei Viruserkrankungen unter besonderer Berücksichtigung der Strahlenbelastung. Rep. to the Min. of Def. (Insan), (1979).
Hochstein-Mintzel, V., Hänichen, T., Huber, H. C., Stickl, H.: Vaccinia- and variolaprotektive Wirkung des modifizierten Vaccinia-Stammes MVA bei intramuskulärer Immunisierung. Zbl. Bakt. Hyg., I. Abt. Orig.A230, 283–297 (1975).
Jentzsch, U., Rau, G., Weitzenegger, E.: Dose measurements in a dummy for the irradiation of test animals in a reactor. Kerntechn.20, 61–69 (1978).
Kundin, W. D.: A pilot study to investigate the effects of x-irradiation on the course of pathogenesis of viral disease as studied by immunofluorescence. TID-20 774 (1964).
Mayr, A.: Experimentelle Arbeiten über das hämagglutinierende Prinzip bei den Tierpockenviren. Arch. ges. Virusforsch.6, 439–481 (1955).
Petersen, P. O., Berenzina, O. N., Kozlova, J. A.: The effect of ionizing radiation on virus infection and antivirus immunity. USAEC Report, AEC-tr-5579 (1963).
Quintans, J., Kaplan, D.: Differential effects of sublethal irradiation on the humoral immune response of adult and neonatal mice. Cell. Immunol.40, 236 to 242 (1978).
Shafikova, R. A., Marennikova, S. S.: Comparative studies on the properties of variola virus strain II. Acta virol.15, 321–323 (1971).
Simon, J., Hochstein-Mintzel, V., Huber, H. C.: Suppression der experimentellen allergischen Encephalomyelitis durch Vaccinia-virus induzierte Hirnantikörper. Z. Immun. Forsch.152, 23–35 (1977).
Simon, J., Werner, G. T.: Vaccinia Virus infection of the CNS in x-irradiated mice. Infect. Immun.25, 1035–1042 (1979).
Stickl, H., Hochstein-Mintzel, V., Mayr, A., Huber, H. C., Schäfer, H., Holzner, A.: MVA-Stufenimpfung gegen Pocken, Deutsch. med. Wschr.99, 2386–2392 (1974).
Svet-Moldavskaya, I. A., Chimishkyan, K. I.: “Vaccinial disease” of mice. Acta Virol.13, 557–559 (1969).
Tagliaferro, W. H., Tagliaferro, L. G., Jaroslow, B. N.: Radiation and immune mechanisms. New York: Academic Press 1964.
Tarro, G., Sabin, A. B.: Increase in preexisting cellular antigencombining groups at different times after infection with different viruses. Proc. Nat. Acad. Sci. Wash.67, 631–737 (1970).
Ueda, Y., Ito, M., Tagaya, I.: A specific surface antigen induced by poxvirus. Virology39, 180–182 (1969).
Wustrow, T., Werner, G. T., Wagner, N., Messerschmidt, O.: Untersuchungen über Kombinationsschäden. Immunität und Antikörperspiegel bei Kaninchen, die durch Ganzkörperbestrahlung in Kombination mit einer Hautwunde behandelt wurden. Strahlenther. (in press).
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Dedicated to Prof. Dr. Dr. Dr. h. c. R. Haas at his 70th birthday.
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Werner, G.T., Jentzsch, U., Metzger, E. et al. Studies on poxvirus infections in irradiated animals. Archives of Virology 64, 247–256 (1980). https://doi.org/10.1007/BF01322704
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DOI: https://doi.org/10.1007/BF01322704