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Comparison of the entire nucleotide and deduced amino acid sequences of the attenuated hog cholera vaccine strain GPE− and the wild-type parental strain ALD

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Summary

We have determined the complete nucleotide sequences of a live attenuated hog cholera virus (HCV) and its progenitor strain. The viral RNA of each strain consisted of 12 298 nucleotides including untranslated regions of 373 and 228 bases at the 5′ and 3′ end, respectively. There was a single large open reading frame spanning 11 697 nucleotides which could encode a large protein of 3 899 amino acids with a calculated molecular weight of 438-kDa. We have found 225 nucleotide difference between the two strains, of which six were located in the untranslated region. Four-sixths of these differences resulted in amino acid substitutions.

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References

  1. Bazab JF, Fletterick RJ (1989) Detection of a trypsin-like serine protease domain in flaviviruses and pestivirus. Virology 171: 637–639

    Google Scholar 

  2. Brock KV (1991) Detection of persistent bovine viral diarrhea virus infections by DNA hybridization and polymerase chain reaction assay. In: Liess B, Moennig V, Pohlenz J, Trautwein G (eds) Ruminant pestivirus infections. Virology, pathogenesis and perspectives. Springer, Wien New York, pp 199–208 (Arch Virol [Suppl] 3)

    Google Scholar 

  3. Collett MS, Larson R, Gold C, Strinck D, Anderson DK, Purchio AF (1988) Molecular cloning and nucleotide sequence of the pestivirus bovine viral diarrhea virus. Virology 165: 191–199

    Google Scholar 

  4. Darbyshire JH (1960) A serological relationship between swine fever and mucosal disease of cattle. Vet Rec 72: 332–333

    Google Scholar 

  5. Deng R, Brock KV (1992) Molecular cloning and nucleotide sequence of a pestivirus genome, noncytopathic bovine viral diarrhea virus strain SD-1. Virology 191: 867–879

    Google Scholar 

  6. Dinter Z (1963) Relationship between bovine viral diarrhea virus and hog cholera virus. Zentralbl Mikrobiol Hyg A 188: 475–486

    Google Scholar 

  7. Edwards JBDM, Delort J, Mallet J (1991) Oligodeoxyribonucleotide ligation to single-strand cDNAs-A new tool for cloning 5′-ends of messenger RNAs and for constructing cDNA libraries by invitro amplification. Nucleic Acids Res 19: 5227–5232

    Google Scholar 

  8. Edwards S, Moennig V, Wensvoort G (1991) The development of an international reference panel of monoclonal antibodies for the differentiation of hog cholera virus from other pestiviruses. Vet Microbiol 29: 101–108

    Google Scholar 

  9. Hahn CS, Dalrymple JM, Strauss JH, Rice CM (1987) Comparison of the virulent Asibi strain yellow fever with the 17D vaccine strain derived from it. Proc Natl Acad Sci USA 84: 2019–2023

    Google Scholar 

  10. Lipton HL, Calenoff M, Bandyopadhyay P, Miller SD, Canto MCD, Gerety S, Jensen K (1991) The 5′ noncoding sequences from a less virulent Theiler's virus dramatically attenuate GDVII neurovirulence. J Virol 65: 4370–4377

    Google Scholar 

  11. Lusting S, Jackson AC, Hahn CS, Griffin DE, Strauss EG, Strauss JH (1988) Molecular basis of Sindbis virus neurovirulence in mice. J Virol 62: 2329–2336

    Google Scholar 

  12. Macadam AJ, Pollard SR, Ferguson G, Dunn G, Skkuce R, Almond JW, Minor PD (1991) The 5′ noncoding region of the type 2 poliovirus vaccine strain contains determinants of attenuation and temperature sensitivity. Virology 181: 451–458

    Google Scholar 

  13. Meyers G, Rümenapf T, Thiel H-J (1989) Molecular cloning and nucleotide sequence of the genome of hog cholera virus. Virology 171: 555–567

    Google Scholar 

  14. Moormann RJM, Petra A, Warmerdam M, Bert VDM, Wim M, Schaaper M, Wensvoort G, Hulst MM (1990) Molecular cloning and nucleotide sequence of hog cholera virus strain Brescia and mapping of the genomic region encoding envelope protein E1. Virology 177: 184–198

    Google Scholar 

  15. Nomoto A, Omata T, Toyoda H, Kuge S, Horie H, Kataoka Y, Genba Y, Nakano Y, Imura N (1982) Complete nucleotide sequence of the attenuated poliovirus Sabin 1 strain genome. Proc Natl Acad Sci USA 79: 5793–5797

    Google Scholar 

  16. Okaniwa A, Nikagawa M, Shimizu Y, Furuuchi S (1969) Lesions in swine inoculated with attenuated hog cholera virus. Nat Inst Anim Hlth Q 9: 92–103

    Google Scholar 

  17. Pilipenko E, Blinov VM, Romanova LI, Sinyakov AN, Moslova SV, Agol VI (1989) Conserved structural domains in the 5′-untranslated region of picornaviral genomes: an analysis of the segment controlling translation and neurovirulence. Virology 168: 201–219

    Google Scholar 

  18. Polo JM, Johnston RE (1991) Mutational analysis of virulence locus in the E2 glycoprotein gene of Sindbis virus. J Virol 65: 6358–6361

    Google Scholar 

  19. Renard A, Dina D, Martinal JA (1987) Vaccines and diagnostics derived from bovine viral diarrhea virus. European patent application number 86870095.6. Publication number 0208672, 14 January 1987

  20. Rümenapf T, Unger G, Stauss JH, Thiel H-J (1993) Processing of the envelope glycoproteins of pestiviruses. J Virol 67: 3288–3294

    Google Scholar 

  21. Shimizu Y, Furuuchi S, Kumagai T, Sasahara J (1970) A mutant of hog cholera virus inducing interference in swine testicle cell cultures. Am J Vet Res 31: 1789–1794

    Google Scholar 

  22. Snyder MH, Betts RF, DeBorde D, Tierney EL, Clements ML, Herrington D, Sears SD, Dolin R, Maassab HF, Murphy BR (1988) Four viral genes independently contribute to attenuation of live influenza A/Ann Arbor/6/60 (H2N2) cold-adapted reassortant virus vaccines. J Virol 62: 448–495

    Google Scholar 

  23. Stark R, Meyers G, Rümenapf T, Thiel H-J (1993) Processing of pestivirus polyprotein: cleavage site between autoprotease and nucleocapsid protein of classical swine fever virus. J Virol 67: 7088–7095

    Google Scholar 

  24. Stokes A, Tierney EL, Sarris CM, Murphy BR, Hall SL (1993) The complete nucleotide sequences of two cold-adapted, temperature-sensitive attenuated mutant vaccine viruses (cp12 and cp45) derived from the JS strains of human parainfluenza virus type 3 (PIV 3). Virus Res 30: 43–52

    Google Scholar 

  25. Van Oirschot J (1986) Hog cholera. In: Leman AD, Straw B, Glock RD, Mengeling WL, Penny RHC, School E (eds) Diseases of swine. Iowa State University Press, Ames, pp 289–300

    Google Scholar 

  26. Westaway EG, Brinton MA, Gaidamovich SYA, Horzinek MC, Igarashi A, Kääriönen L, Lvov DK, Porterfield JS, Russel PK, Trent DW (1985) Togaviridae. Intervirology 24: 125–139

    Google Scholar 

  27. Westrop GD, Wareham KA, Evans DMA, Dunn G, Minor PD, McGrath DI, Traffs F, Marsden S, Skinner MA, Schild GC, Almond JW (1989) Genetic basis of attenuation of the Sabin type 3 oral poliovirus vaccine. J Virol 63: 1338–1344

    Google Scholar 

  28. Wiskerchen M, Collett MS (1991) Pestivirus gene expression: protein p80 of bovine viral diarrhea virus is a proteinase involved in polyprotein processing. Virology 184: 1–10

    Google Scholar 

  29. Yamada A, Takeuchi K, Tanabayashi K, Hishiyama M, Sugiura A, (1989) Sequence variation of the P gene among mumps virus strains. Virology 172: 374–376

    Google Scholar 

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Authors and Affiliations

  1. National Veterinary Assay Laboratory, Tokyo

    K. Ishikawa, H. Nagai, M. Tsutsui, A. Saitoh, M. Takagi, K. Gotoh & M. Muramatsu

  2. Basic Research Division, Biomedical Laboratories, Inc., Saitama

    K. Katayama

  3. Department of Viral Disease and Vaccine Control, National Institute of Health, Tokyo, Japan

    K. Tanabayashi, K. Takeuchi, M. Hishiyama & A. Yamada

Authors
  1. K. Ishikawa
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  2. H. Nagai
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  3. K. Katayama
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  4. M. Tsutsui
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  5. K. Tanabayashi
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  6. K. Takeuchi
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  7. M. Hishiyama
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  8. A. Saitoh
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  9. M. Takagi
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  10. K. Gotoh
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  11. M. Muramatsu
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  12. A. Yamada
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Ishikawa, K., Nagai, H., Katayama, K. et al. Comparison of the entire nucleotide and deduced amino acid sequences of the attenuated hog cholera vaccine strain GPE− and the wild-type parental strain ALD. Archives of Virology 140, 1385–1391 (1995). https://doi.org/10.1007/BF01322665

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  • DOI: https://doi.org/10.1007/BF01322665

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