Summary
Black locust (Robinia pseudoacacia L.), a member of the legume tribe Robinieae, has indeterminate root nodules and is primarily an amide exporter. However, in greenhouse-grown seedlings inoculated with rhizobia, ureide-N comprises approximately 8% of the total soluble-N in the xylem sap. Ultrastructurally, young interstitial cells (i.e., uninfected cells in the infected region near the nodule meristem) develop enlarged peroxisomes and abundant tubular ER, properties heretofore found to be characteristic only of members of the Phaseoleae, which have determinate nodules and are exporters principally of ureides. Many of the peroxisomes in the interstitial cells of black locust nodules react strongly for uricase (EC 1.7.3.3) activity in a cytochemical test employing diaminobenzidine, but some of the peroxisomes, particularly those farther back from the meristem, react weakly or not at all, even though enlarged. The ultrastructural specializations of the interstitial cells seem disproportionately large in comparison to the relatively low levels of ureides in the xylem transport stream. We suggest that similar specialization of some of the interstitial cells might be found in other legumes that export low amounts of ureides.
Similar content being viewed by others
Abbreviations
- DAB:
-
3,3'diaminobenzidine
- ER:
-
endoplasmic reticulum
References
Allen ON, Allen EK (1981) The leguminosae. A source book of characteristics, uses and nodulation. University of Wisconsin Press, Madison
Hanks JF, Tolbert NE, Schubert KR (1981) Localization of enzymes of ureide biosynthesis in peroxisomes and microsomes of nodules. Plant Physiol 68: 65–69
Hoagland DR, Snyder WC (1933) Nutrition of strawberry plant under controlled conditions. Proc Am Soc Hort Sci 30: 288–349
McClure PR, Israel DW (1979) Transport of nitrogen in the xylem of soybean plants. Plant Physiol 64: 411–416
Mothes K (1961) The metabolism of urea and ureides. Can J Bot 39: 1785–1806
Newcomb EH, Tandon SR (1981) Uninfected cells of soybean root nodules: ultrastructure suggests key role in ureide production. Science 212: 1394–1396
— —, Kowal RR (1985) Ultrastructural specialization for ureide production in uninfected cells of soybean root nodules. Protoplasma 125: 1–12
Reinbothe H, Mothes K (1962) Urea, ureides and guanidines in plants. Annu Rev Plant Physiol 13: 129–150
Schubert KR (1981) Enzymes of purine synthesis and catabolism inGlycine max. I. Comparison of activities with N2 fixation and composition of xylem exudate during nodule development. Plant Physiol 68: 1115–1122
—, Boland MJ (1990) The ureides. In: Miflin BJ (ed) The biochemistry of plants, vol 15. Academic Press, New York (in press)
Shelp BJ, Atkins CA, Storer PJ, Canvin DT (1983) Cellular and subcellular organization of pathways of ammonia assimilation and ureide synthesis in nodules of cowpea [(Vigna unguiculata) Walp.]. Arch Biochem Biophys 224: 429–441
Thomas RJ, Feller U, Erismann KH (1979) The effect of different inorganic nitrogen sources and plant age on the composition of bleeding sap ofPhaseolus vulgaris. New Phytol 82: 657–669
Vogels GD, Van der Drift C (1970) Differential analysis of glyoxylate derivatives. Anal Biochem 33: 143–157
Yokota S, Nagata T (1977) Urate oxidase. In: Hayat MA (ed) Electron microscopy of enzymes, vol 5. Van Nostrand Reinhold, New York, pp 72–97
Webb MA, Newcomb EH, Schubert KR (1987) Purification of allantoinase from soybean root nodules. Plant Physiol 83: 21
Author information
Authors and Affiliations
Additional information
Dedicated to the memory of Professor Oswald Kiermayer
Rights and permissions
About this article
Cite this article
Kaneko, Y., Newcomb, E.H. Specialization for ureide biogenesis in the root nodules of black locust (Robinia pseudoacacia L.), an amide exporter. Protoplasma 157, 102–111 (1990). https://doi.org/10.1007/BF01322642
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01322642