Skip to main content
SpringerLink
Log in

Short-term effects of organic silicon on trabecular bone in mature ovariectomized rats

  • Laboratory Investigations
  • Published:
Calcified Tissue International Aims and scope Submit manuscript

Summary

Silicon is known to ensure an essential role in the formation of cross-links between collagen and proteoglycans during bone growth. In this study, we have evaluated the short-term effects of a preventive treatment with silanol, a soluble organic silicon (Si), on trabecular bone in mature ovariectomized rats. Three-month-old rats were shamoperated (sham) or were ovariectomized (OVX) and treated with 10 μg/kg/day of 170 estradiol (E2), or with 0.1 mg Si/kg/day or 1.0 mg Si/kg/day of silanol for 1 month. Plasma alkaline phosphatase and osteocalcin levels were increased by 50% in OVX rats compared with sham rats and were corrected by E2 but not by silanol treatment. The trabecular bone volume measured at the tibial metaphysis was decreased by 48%, and histomorphometric indices of bone resorption and formation were increased in OVX rats compared with sham, and these parameters were corrected by E2 treatment. Treatment of OVX rats with silanol decreased the osteoclast surface by 31% and the number of osteoclasts by 20%. The mineral apposition rate, the bone formation rate, and the osteoblast surface at the tibia metaphyseal area were increased by 30% at the higher dose of silanol compared with OVX rats. In contrast, silanol treatment had no effect on the periosteal apposition rate. The reduction of the metaphyseal bone resorption and the increased bone formation induced by silanol resulted in a slight improvement of the trabecular bone volume (+ 14%) compared with controls. The results indicate that a short-term preventive treatment with the organic silicon silanol partially prevented the trabecular bone loss in mature OVX rats by reducing bone resorption and increasing bone formation, possibly through stimulatory effects on the formation and/or the stability of the organic bone matrix.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Parfitt AM (1984) Age-related structural changes in trabecular and cortical bone: cellular mechanisms and biochemical consequences. Calcif Tissue Int 36:S123-S128

    Google Scholar 

  2. Wronski TJ, Cintron M, Doherty AL, Dann LM (1988) Estrogen treatment prevents osteopenia and depresses bone turnover in ovariectomized rats. Endocrinology 123:681–686

    Google Scholar 

  3. Wronski TJ, Dan LM, Scott KS, Crooke LIZ (1989) Endocrine and pharmacological suppressors of bone turnover protect against osteopenia in ovariectomized rats. Endocrinology 125:810–816

    Google Scholar 

  4. Hayashi T, Yamamuro T, Okumurah, Kasai R, Toda K (1989) Effect of eel calcitonin on the prevention of osteoporosis induced by combination of immobilization and ovariectomy in the rat. Bone 10:25–28

    Google Scholar 

  5. Wronski TJ, Yen CF, Burton KW, Mehta RC, Newman PS, Soltis EE, DeLuca PP (1991) Skeletal effects of calcitonin in ovariectomized rats. Endocrinology 129:2246–2250

    Google Scholar 

  6. Hock JM, Gera I, Fonseca J, Raisz LG (1988) Human PTH (1–34) increases bone in ovariectomized and orchidectomized rats. Endocrinology 122:2899–2903

    Google Scholar 

  7. Hori M, Usawa T, Morita K, Noda T, Takahashi H, Inoue J (1988) Effect of human parathyroid hormone (PTH 1–34) on experimental osteopenia of rats induced by ovariectomy. J Bone Miner Res 3:193–199

    Google Scholar 

  8. Modrowski D, Miravet L, Feuga M, Bannié F, Marie PJ (1992) Effect of fluoride on bone and bone cells in ovariectomized rats. J Bone Miner Res 7:961–969

    Google Scholar 

  9. Ke HZ, Li M, Jee WSS (1992) Prostaglandin E2 prevents ovariectomy-induced cancellous bone loss in rats. Bone Miner 19:45–62

    Google Scholar 

  10. Mori S, Jee WSS, Li XJ (1992) Production of new trabecular bone in osteopenic ovariectomized rats by prostaglandin E2. Calcif Tissue Int 50:80–87

    Google Scholar 

  11. Marie PJ, Hott M, Modrowski D, De Pollack C, Guillemain J, Deloffre P, Tsouderos Y (1993) An uncoupling agent containing strontium (S 12911) prevents bone loss by depressing bone resorption and maintaining bone formation in estrogen-deficient rats. J Bone Miner Res 8:607–615

    Google Scholar 

  12. Laponche AM, Corriol S, Chauvin M, Eisinger J (1977) Osteoporoses: technique d'exploration et therapeutique nouvelle. Lyon Mediter Med 13:1349–1351

    Google Scholar 

  13. Schiano A, Eisinger F, Detolle P, Laponche AM, Brisou B, Eisinger J (1979) Silicium, tissu osseux et immunité. Rev Rhumat 46:483–486

    Google Scholar 

  14. Schwarz K (1973) A bound form of silicon in glycosaminoglycans and polyuronides. Proc Natl Acad Sci 70:1608–1612

    Google Scholar 

  15. Carlisle EM (1976) In vivo requirement for silicon in articular cartilage and connective tissue formation in the chick. J Nutr 106:478–484

    Google Scholar 

  16. Carlisle EM (1981) Silicon in bone formation, vol 4. In: Simpson TL, Volcani BE (eds) Springer Verlag, New York pp 69–94

    Google Scholar 

  17. Allain P, Cailleux A, Mauras Y, Renier JC (1983) Etude de l'absorption digestive du silicium après administration unique chez l'homme sous forme de salicylate de methylsilanetriol. Therapie 38:171–174

    Google Scholar 

  18. Kalu DN (1991) The ovariectomized rat model of postmenopausal bone loss. Bone Miner 15:175–192

    Google Scholar 

  19. Wronski TJ, Yen C-F (1991) The ovariectomized rat as an animal model for postmenopausal bone loss. Cells Mater S1:69–74

    Google Scholar 

  20. Wronski TJ, Schenck PA, Cintron M, Walsh CC (1987) Effect of body weight on osteopenia in ovariectomized rats. Calcif Tissue Int 40:155–159

    Google Scholar 

  21. Modrowski D, Del Pozo E, Miravet L (1992) Dynamics of circulating osteocalcin in rats during growth and under experimental conditions. Horm Met Res 24:474–477

    Google Scholar 

  22. Baron R, Vignery A, Neff L, Silverglate A, Maria AS (1983) Processing of undecalcified bone specimens for bone histomorphometry. In: Recker RR (ed) Bone histomorphometry. CRC Press Inc, Boca Raton, FL, pp 13–36

    Google Scholar 

  23. Parfitt AM, Drezner MK, Glorieux FH, Kanis JA, Malluche HH, Meunier PJ, Ott SM, Recker RR (1987) Bone histomorphometry: standardization of nomenclature, symbols and units. J Bone Miner Res 6:595–610

    Google Scholar 

  24. Turner TT, Vandersteenhoven JJ, Bell NH (1984) The effect of ovariectomy and l7β estradiol on cortical bone histomorphometry in growing rats. J Bone Miner Res 2:115–122

    Google Scholar 

  25. Wronski TJ, Cintron M, Dann LM (1988) Temporal relationship between bone loss and increased bone turnover in ovariectomized rats. Calcif Tissue Int 43:179–183

    Google Scholar 

  26. Chow JWM, Lean JM, Chambers TJ (1992) 17β-estradiol stimulates cancellous bone formation in female rats. Endocrinology 130:3025–3082

    Google Scholar 

  27. Turner RT, Colvard DS, Spelsberg TC (1990) Estrogen inhibition of periosteal bone formation in rat long bones: downregulation of gene expression for bone matrix proteins. Endocrinology 127:1346–1351

    Google Scholar 

  28. Eisinger J, Clairet D, Zakarian H, Ouaniche J, Beyssac M, Arroyo P, Arroyo H (1990) Evolution a court terme des osteoporoses traitées: étude absorptiométrique. Médecine du Sud Ouest 26:323–325

    Google Scholar 

  29. Keeting PE, Oursler MJ, Wiegand KE, Bonde SK, Spelsberg TC, Riggs BL (1992) Zeolite A increases proliferation, differentiation and transforming growth factor β production in normal human adult osteoblast-like cells in vitro. J Bone Miner Res 7:1281–1289

    Google Scholar 

  30. Brady MC, Dobson PRM (1991) Zeolite A stimulates proliferation and protein synthesis in human osteoblast-like cells and the osteosarcoma cell line MG 63. J Bone Miner Res 6:S139

    Google Scholar 

  31. Modrowski D, Miravet L, Feuga M, Marie PJ (1993) Increased proliferation of osteoblast precursor cells in estrogen-deficient rats. Am J Physiol 264 (Endocrinol Metab 27):E190-E196

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Unité 349 INSERM, Cell and Molecular Biology of Bone and Cartilage, Hôpital Lariboisière, 6 rue Guy Patin, 75010, Paris, France

    Monique Hott, Cinderella de Pollak, Dominique Modrowski & Pierre J. Marie

Authors
  1. Monique Hott
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Cinderella de Pollak
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Dominique Modrowski
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Pierre J. Marie
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hott, M., de Pollak, C., Modrowski, D. et al. Short-term effects of organic silicon on trabecular bone in mature ovariectomized rats. Calcif Tissue Int 53, 174–179 (1993). https://doi.org/10.1007/BF01321834

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01321834

Key words

Search

Navigation