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In situ localisation of beet necrotic yellow vein virus (BNYVV) in rootlets of susceptible and resistant beet plants

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Summary

Mechanisms of resistance to beet necrotic yellow vein virus (BNYVV) were studied by comparing the multiplication and distribution of BNYVV in root tissue of some beet accessions. Seedlings were infected either by soil containing resting spores ofPolymyxa betae with BNYVV, or by a viruliferous zoospore suspension. With both inoculation methods high virus concentrations were obtained in rootlets of the susceptible cultivar ‘Regina’. Using infested soil, low virus concentrations were found in the partially resistant cultivar ‘Rima’ and in the resistant accessions Holly and WB42. When a zoospore suspension was used, similar virus concentrations occurred in ‘Rima’ and Holly as in ‘Regina’, while a low virus concentration was found in WB42. By in situ localisation studies, using immunogold-silver labelling, virus was detected in ‘Regina’ after infection by soil or a zoospore suspension, but it could only be detected in the resistant accessions after infection by a zoospore suspension. In rootlets of ‘Regina’, ‘Rima’ and Holly, virus was found in the epidermis, cortex parenchyma, endodermis, and interstitial parenchyma, but in general not inside the vascular tissue. In WB42 the virus, occurring in small aggregates, seemed to be restricted to the epidermis and some cortex parenchyma cells. Comparing both the multiplication and distribution of BNYVV in rootlets of the accessions studied, it is concluded that the virus resistance mechanism in ‘Rima’ and Holly is different from that in WB42.

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Authors and Affiliations

  1. DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), Wageningen, The Netherlands

    O. E. Scholten & H. Paul

  2. Department of Virology, Wageningen Agricultural University, Wageningen, The Netherlands

    D. Peters, J. W. M. Van Lent & R. W. Goldbach

Authors
  1. O. E. Scholten
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  2. H. Paul
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  3. D. Peters
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  4. J. W. M. Van Lent
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  5. R. W. Goldbach
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Scholten, O.E., Paul, H., Peters, D. et al. In situ localisation of beet necrotic yellow vein virus (BNYVV) in rootlets of susceptible and resistant beet plants. Archives of Virology 136, 349–361 (1994). https://doi.org/10.1007/BF01321063

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