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Immunogold localization of light-harvesting and photosystem I complexes in the thylakoids ofFucus serratus (Phaeophyceae)

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Summary

The repartition of light-harvesting complex (LHC) and photosystem I (PS I) complex has been examined in isolated plastids ofFucus serratus by immunocytochemical labelling. LHC is distributed equally all along the length of thylakoid membranes, without any special repartition in the appressed membranes of the three associated thylakoids ofFucus. PS I is present on all the thylakoid membranes, but the external membranes of the three associated thylakoids are largely enriched relatively to the inner ones. This specific repartition of PSI on non-appressed membranes can be compared to the localization of PSI on stroma thylakoid membranes of higher plants and green algae. Consequently, although they share some common features with those of higher plants and green algae, the appressions of thylakoids in brown algae has neither the same structure nor the same functional role as typical grana stacked membranes in the repartition of the harvested energy.

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Abbreviations

BSA:

bovine serum albumin

GAR:

goat anti-rabbit immunoglobulin G

LHC:

light-harvesting complex

PBS:

phosphatebuffered saline

PS I:

photosystem I

PS II:

photosystem II

References

  • Alberte RS, Friedman AL, Gustafson DL, Rudnick MS, Lyman H (1981) Light-harvesting systems of brown algae and diatoms. Isolation and characterization of chlorophylla/c and chlorophylla/fucoxanthin pigment-protein complexes. Biochim Biophys Acta 635: 304–316

    Google Scholar 

  • Anderson JM, Barrett J (1986) Light-harvesting pigment-protein complexes of algae. In: Staehelin LA, Arntzen CJ (eds) Photosynthesis III. Photosynthetic membranes and light-harvesting systems. Springer, Berlin Heidelberg New York Tokyo, pp 269–284 [Pirson A, Zimmermann MH (eds) Encyclopedia of plant physiology, NS, vol 19]

    Google Scholar 

  • Andersson B, Anderson JM (1980) Lateral heterogeneity in the distribution of chlorophyll-protein complexes of the thylakoid membranes of spinach chloroplasts. Biochim Biophys Acta 593: 427–440

    Google Scholar 

  • Armond PA, Staehelin LA, Arntzen CJ (1977) Spatial relationship of photosystem I, photosystem II, and the light-harvesting complex in chloroplast membranes. J Cell Biol 73: 400–418

    Google Scholar 

  • Barrett J, Anderson JM (1977) Thylakoid membrane fragments with different chlorophylla, chlorophyllc and fucoxanthin compositions isolated from the brown seaweedEcklonia radiata. Plant Sci Lett 9: 275–283

    Google Scholar 

  • Bassi R, Simpson D (1987) Chlorophyll-protein complexes of barley photosystem I. Eur J Biochem 163: 221–230

    Google Scholar 

  • —, Rigoni F, Giacometti GM (1990) Chlorophyll binding proteins with antenna function in higher plants and green algae. Photochem Photobiol 52: 1187–1206

    Google Scholar 

  • Berkaloff C, Duval JC (1980) Comparative study between green plant and brown alga chloroplasts. Photosynthesis Res 1: 127–135

    Google Scholar 

  • —, Caron L, Rousseau B (1990) Subunit organization of PSI particles from brown algae and diatoms: polypeptide and pigment analysis. Photosynthesis Res 23: 181–193

    Google Scholar 

  • —, Duval JC, Hauswirth N, Rousseau B (1983) Freeze-fracture study of thylakoids ofFucus serratus. J Phycol 19: 96–100

    Google Scholar 

  • — —, Rousseau B (1984) Active PS II particles from a brown alga: spectral and electrophoretic characterization. In: Sybesma S (ed) Advances in photosynthesis research, vol 1. Martinus Nijhoff/ Dr W Junk, The Hague, pp 449–452

    Google Scholar 

  • Callahan FE, Wergin WP, Nelson N, Edelman M, Mattoo AK (1989) Distribution of thylakoid proteins between stromal and granal lamellae inSpirodela. Dual location of photosystem II components. Plant Physiol 91: 629–635

    Google Scholar 

  • Caron L, Dubacq JP, Berkaloff C, Jupin H (1985) Subchloroplast fractions from the brown algaFucus serratus: phosphatidylglycerol contents. Plant Cell Physiol 26: 131–139

    Google Scholar 

  • —, Rémy R, Berkaloff C (1988) Polypeptide composition of lightharvesting complexes from some brown algae and diatoms. FEBS Lett 229: 11–15

    Google Scholar 

  • Duval JC, Jupin H, Berkaloff C (1983) Photosynthetic properties of plastids isolated from macrophytic brown seaweeds. Physiol Veg 6: 1145–1157

    Google Scholar 

  • Fawley MW, Grossman AR (1986) Polypeptides of light-harvesting complex of the diatomPheodactylum tricornutum are synthesized in the cytoplasm of the cell as precursors. Plant Physiol 81: 149–155

    Google Scholar 

  • Gibbs SP (1970) The comparative ultrastructure of the algal chloroplast. Ann N Y Acad Sci 175: 454–473

    Google Scholar 

  • Goodchild DJ, Andersson B, Anderson JM (1985) Immunocytochemical localization of polypeptides associated with the oxygen evolving system of photosynthesis. Eur J Cell Biol 36: 294–298

    Google Scholar 

  • Grebvy C, Axelsson L, Sundqvist C (1989) Light-independent plastid differentiation in the brown algaLaminaria saccharina (Phaeophyceae). Phycologia 28: 375–384

    Google Scholar 

  • Lam E, Ortiz W, Mayfield S, Malkin R (1984) Isolation and characterization of a light-harvesting chlorophylla/b protein complex associated with photosystem I. Plant Physiol 74: 650–655

    Google Scholar 

  • Lichtlé C, McKay RML, Gibbs SP (1992) Immunogold localization of photosystem I and of photosystem II light-harvesting complexes in cryptomonad thylakoids. Biol Cell (in press)

  • —, Passaquet C, Thomas JC, Lemoine Y (1989) Immunological studies on light-harvesting complexes of brown algae. Physiol Plantarum 76: p 78

    Google Scholar 

  • Ludwig M, Gibbs SP (1989) Localization of phycoerythrin at the lumenal surface of the thylakoid membrane inRhodomonas lens. J Cell Biol 108: 875–884

    Google Scholar 

  • Owens TG (1986) Light-harvesting function in the diatomPheodactylum tricornutum. II Distribution of excitation energy between the two photosystems. Plant Physiol 80: 739–746

    Google Scholar 

  • Passaquet C, Thomas JC, Caron L, Hauswirth N, Puel F, Berkaloff C (1991) Light-harvesting complexes of brown algae: biochemical characterization and immunological relationships. FEBS Lett 280: 21–26

    Google Scholar 

  • Rhiel E, Kunz J, Wehrmeyer W (1989) Immunocytochemical localization of phycoerythrin 545 and of a chlorophylla/c lightharvesting complex inCryptomonas maculata (Cryptophyceae). Bot Acta 102: 46–53

    Google Scholar 

  • Roth J (1982) The protein A-gold (pAg) technique-a qualitative and quantitative approach for antigen localization on thin sections. In: Bullock GR, Petruz P (eds) Techniques in immunocytochemistry, vol 1. Academic Press, New York, pp 107–133

    Google Scholar 

  • Spear-Bernstein L, Miller KR (1989) Unique location of the phycobiliprotein light-harvesting pigment in the Cryptophyceae. J Phycol 25: 412–419

    Google Scholar 

  • Staehelin LA (1986) Chloroplast structure and supramolecular organization of photosynthetic membranes. In: Staehelin LA, Arntzen CJ (eds) Photosynthesis III. Photosynthetic membranes and light-harvesting systems. Springer, Berlin Heidelberg New York Tokyo, pp 1–84 [Pirson A, Zimmermann MH (eds) Encyclopedia of plant physiology, NS, vol 19]

    Google Scholar 

  • Thornber JP (1986) Biochemical characterization and structure of pigment-proteins of photosynthetic organisms. In: Staehelin LA, Arntzen CJ (eds) Photosynthesis III. Photosynthetic membranes and light-harvesting systems. Springer, Berlin Heidelberg New York Tokyo, pp 98–142 [Pirson A, Zimmermann MH (eds) Encyclopedia of plant physiology, NS, vol 19]

    Google Scholar 

  • Towbin H, Staehelin LA, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76: 4350–4354

    Google Scholar 

  • Vallon O, Wollman FA, Olive J (1985) Distribution of intrinsic and extrinsic subunits of the PS II protein complex between appressed and non-appressed regions of the thylakoid membrane: an immunocytochemical study. FEBS Lett 183: 245–250

    Google Scholar 

  • Vallon O, Wollman FA, Olive J (1986) Lateral distribution of the main complexes of the photosynthetic apparatus inChlamydomonas reinhardtii and in spinach: an immunocytochemical study using intact thylakoid membranes and a PS II enriched membrane preparation. Photobiochem Photobiophys 12: 203–220

    Google Scholar 

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Authors and Affiliations

  1. Laboratoire des Biomembranes et Surfaces Cellulaires Végétales, (UA C.N.R.S. DO 311), Ecole Normale Supérieure, Paris

    Christiane Lichtlé, Agnès Spilar & J. C. Duval

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  1. Christiane Lichtlé
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  2. Agnès Spilar
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  3. J. C. Duval
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Lichtlé, C., Spilar, A. & Duval, J.C. Immunogold localization of light-harvesting and photosystem I complexes in the thylakoids ofFucus serratus (Phaeophyceae). Protoplasma 166, 99–106 (1992). https://doi.org/10.1007/BF01320148

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  • DOI: https://doi.org/10.1007/BF01320148

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