Summary
Cells infected with four strains (LEC, Biken, IP-3 and DR) of subacute sclerosing panencephalitis (SSPE) virus were compared with wild type measles virus (Edmonston) with respect to titers of extracellular virus, morphology of the cytopathic effect (CPE) and occurrence of different measles virus antigens within infected cells as determined by immune fluorescence. Murine monoclonal antibodies with specifities for the nucleocapsid (NP), polymerase (P), matrix (M), hemagglutinin (H), and fusion (F) proteins as well as specific hyperimmune sera prepared in rabbits against the NP, H and M proteins were used in immune fluorescence analyses of the various strains. All of the strains produced large amounts of NP and P. Only the NP antigen occurred in nuclei of cells. The Edmonston and LEC strains also showed bright fluorescence with the antibodies against the H, F, and M antigens. Immune fluorescent intensity was variably reduced in cells infected with the Biken, IP-3, and DR strains labelled with anti M, H, or F antibodies. The Biken strain produced moderate titers of extracellular virus and moderate amounts of M, H, and F antigens whereas the DR strain produced no extracellular virus and contained no detectable M or F and only trace amounts of H antigen. The IP-3 strain was intermediate both in antigen expression and in production of extracellular virus.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Barbanti-Brodano, G., Oyanagi, S., Katz, M., Koprowski, H.: Presence of two different viral agents in brain cells of patients with subacute sclerosing panencephalitis. Proc. Soc. Exp. Biol. Med.134, 230–236 (1969).
Burnstein, F., Jacobsen, L. B., Zeman, W., Chen, T. T.: Persistent infection of BSC-1 cells by defective measles virus derived from subacute sclerosing panencephalitis. Infect. Immun.10, 1378–1382 (1974).
Byington, D. P., Johnson, K. P.: Experimental subacute sclerosing panencephalitis in the hamster: Correlation of age with chronic inclusion-cell encephalitis. J. Infect. Dis.126, 18–26 (1972).
Carrigan, D. R., Johnson, K. P.: Experimental relapsing myelitis in hamsters associated with a variant of measles virus. In:Fields, B., Jaenisch, R., Fox, C. F. (eds.), Animal Virus Genetics. ICN-UCLA Symposia on Molecular and Cellular Biology, Vol. XVIII, 759–768. New York: Academic Press 1980.
Carrigan, D. R., Johnson, K. P.: A mathematical model for the hemolysis of measles virus. J. Virol. Meth.3, 71–82 (1981).
Carrigan, D. R., McKendall, R. R., Johnson, K. P.: CNS disease following dissemination of SSPE measles virus from intraperitoneal inoculation of suckling hamsters. J. Med. Virol.2, 347–357 (1978).
Hall, W. W., Choppin, P. W.: Evidence for lack of synthesis of the M polypeptide of measles virus in brain cells in subacute sclerosing panencephalitis. Virology99, 443–447 (1979).
Hall, W. W., Choppin, P. W.: Measles virus proteins in the brain tissue of patients with subacute sclerosing panencephalitis: Absence of the M protein. N. Engl. J. Med.304, 1152–1155 (1981).
Hardwick, J. M., Bussell, R. H.: Glycoproteins of measles virus under reducing and non-reducing conditions. J. Virol.25, 687–692 (1978).
Johnson, K. P., Norrby, E.: Subacute sclerosing panencephalitis (SSPE) agent in hamsters. III. Induction of defective measles infection in hamster brain. Exp. Mol. Pathol.21, 166–178 (1974).
Johnson, K. P., Norrby, E., Swoveland, P., Carrigan, D. R.: Experimental subacute sclerosing panencephalitis: Selective disappearance of measles virus matrix protein from the central nervous system. J. Infect. Dis.144, 161–169 (1981).
Lin, F. H., Thormar, H.: Absence of M protein in a cell-associated subacute sclerosing panencephalitis virus. Nature285, 490–492 (1980).
Machamer, C. E., Hayes, E. C., Zweerink, H. J.: Cells infected with a cell-associated subacute sclerosing panencephalitis virus do not express M protein. Virology108, 515–520 (1981).
Miller, C. A., Carrigan, D. R.: Reversible repression and activation of measles virus infection in neural cells. Proc. Nat. Acad. Sci. U.S.A. (in press, 1982).
Norrby, E., Chen, S., Togashi, T., Sheshberadaran, H., Johnson, K. P.: Five measles virus antigens demonstrated by use of mouse hybridoma antibodies in productively infected tissue culture cells. Arch. Virol.71, 1–11 (1982).
Reed, L., Muench, M.: A simple method of estimating fifty percent endpoints. Amer. J. Hyg.27, 493–497 (1938).
Robbins, S. J., Rapp, F.: Inhibition of measles virus replication by cyclic AMP. Virology106, 317–326 (1980).
Stephenson, J. R., Siddell, S. G., ter Meulen, V.: Persistent and lytic infections with SSPE virus: A comparison of the synthesis of virus-specific polypeptides. J. gen. Virol.57, 191–197.
Thormar, H., Jervin, G. A., Karl, S. C., Brown, H. R.: Passage in ferrets of encephalitogenic cell-associated measles virus isolated from brain of a patient with subacute sclerosing panencephalitis. J. Infect. Dis.127, 678–685 (1973).
Togashi, T., Örvell, C., Vartdal, F., Norrby, E.: Production of antibodies against measles virions by use of the mouse hybridoma technique. Arch. Virol.67, 149–157 (1981).
Tyrrell, J. L. D., Rafter, J. D., Örvell, C., Norrby, E.: Isolation and characterization of the nucleocapsid and membrane proteins of measles virus. J. gen. Virol.51, 307–315 (1980).
Ueda, S., Okuno, Y., Hamamoto, Y., Ohya, H.: Subacute sclerosing panencephalitis (SSPE): Isolation of a defective variant of measles virus from brain obtained at autopsy. Biken J.18, 113–122 (1975).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Johnson, K.P., Norrby, E., Swoveland, P. et al. Expression of five viral antigens in cells infected with wild-type and SSPE strains of measles virus: Correlation with cytopathic effects and productivity of infections. Archives of Virology 73, 255–262 (1982). https://doi.org/10.1007/BF01318079
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01318079