Summary
Passage of FMDV in BHK suspended cells was confirmed to induce an antigenic deficiency on the virion. By immunodiffusion experiments with complete virus, with trypsin-treated virus and with 12S virus fraction it was shown that the induced antigenic deficiency is located on the trypsin-removable part of the virion. These results were confirmed by absorption experiments.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Bachrach, H. L., Trautman, R., Breese, S. S.: Chemical and physical properties of virtually pure foot and mouth disease. Amer. vet. Res.25, 333–342 (1964).
Bachrach, H. L., Van de Woude, G. F.: Amino acid composition and C-terminal sequence of foot and mouth disease virus protein. Virology34, 282–289 (1968).
Barteling, S. J.: A simple method for preparation of agarose. Clin. Chem.15, 1002 (1969).
Barteling, S. J.: The use of frozen cells in the agar-cell suspension titration technique. Arch. ges. Virusforsch.38, 271–273 (1972).
Barteling, S. J., Meloen, R. H.: A simple method for the quantification of 140S particles of foot and mouth disease virus. Arch. ges. Virusforsch.45, 362–364 (1974).
Barteling, S. J.: Use of polyethylene glycol-treated serum for production of foot and mouth disease (FMDV) in growing BHK-suspended cell cultures. Bull. Off. int. Epiz.81, 1243–1254 (1974).
Brown, F.: A β-globulin antibody in the sera of guinea pigs and cattle infected with foot and mouth disease. J. Immunol.85, 298–303 (1960).
Brown, F., Smale, C. J.: Demonstration of three specific sites on the surface of foot and mouth disease virus by antibody complexing. J. gen. Virol.7, 115–127 (1970).
Cowan, K. M.: Immunochemical studies of foot and mouth disease. V. Antigenic variants of virus demonstrated by immune diffusion analyses with 19S but not 7S antibodies. J. exp. Med.129, 333–350 (1969).
Cowan, K. M.: Immunochemical studies of foot and mouth disease. VI. Differences in antigenic determinant site recognition by guinea pig 19S and 7S antibodies. J. Immunol.104, 423–431 (1970).
Cowan, K. M., Erol, N., Whiteland, A. P.: Heterogenity of type Asia-1 foot and mouth disease virus and BHK-21 cells and the relationship to vaccine preparation. Bull. Off. int. Epiz.81, 1271–1298 (1974).
Fish, R. C., van Bekkum, J. G., Lehmann, R. P., Richardson, G. V.: Immunologic response in Dutch cattle vaccinated with FMDV vaccins under field conditions. Neutralising antibody responses to O, A and C types. Amer. J. vet. Res.30, 2115–2123 (1969).
Martin, W. B., Chapman, W. G.: Tissue culture colour test for assaying the virus and neutralising antibody of foot and mouth disease and its applications to the measurement of immunity in cattle. Res. vet. Sci.2, 53–61 (1961).
Ouchterlony, O.: Antigen—Antibody reactions in gels. Arkiv. för Kemi, Mineralogi och Geologi26 B, 1 (1949).
Wild, T. F., Brown, F.: Nature of inactivating action of trypsin on foot and mouth disease virus. J. gen. Virol.1, 247–250 (1967).
Wild, T. F., Burroughs, J. N., Brown, F.: Surface structure of Foot-and-Mouth Disease virus. J. gen. Virol.4, 313–320 (1969).
Author information
Authors and Affiliations
Additional information
With 14 Figures
Rights and permissions
About this article
Cite this article
Meloen, R.H. Localisation on foot-and-mouth disease virus (FMDV) of an antigenic deficiency induced by passage in BHK cells. Archives of Virology 51, 299–306 (1976). https://doi.org/10.1007/BF01317933
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01317933