Summary
Venezuelan Equine Encephalitis (Mucambo) virus was able to multiply at 28° C in cultured mosquito cells(Aedes aegypti andAedes albopictus) without inducing any apparent cytopathology. The kinetics of virus multiplication was similar in mosquito and mammalian (Vero) cells. Twelve to twenty four hours post-infection, a maximum of 10–50 per cent of the cells were engaged in virus production; however, almost immediately this proportion fell abruptly tending to stabilize in the order of 0.01 to 0.05 per cent. No significant increase of infectious centers was observed after cell subculturing. Study of subpopulations of mosquito cells revealed a differential suceptibility to virus infection, which suggests that a balance of susceptible and virus resistant cells may play a role in the establishment and persistence of the chronic infection.
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References
Artsob, H., Spence, L.: Growth of Vesicular Stomatitis virus in mosquito cell lines. Canad. J. Microbiol.20, 329–336 (1974).
Artsob, H., Spence, L.: Persistent infection of mosquito cell lines with Vesicular Stomatitis virus. Acta virol.10, 331–340 (1974).
Bergold, G. H., Mazzali, R.: Plaque formation by arboviruses. J. gen. Virol.2, 273–284 (1968).
Bergold, G. H., Suarez, O. M., Munz, K.: Multiplication in and transmission byAedes aegypti of Vesicular Stomatitis virus. J. invertebr. Pathol.11, 406–429 (1968).
Bidwell, D. E.: Multiplication of Semliki Forest virus in the tissues ofAedes aegypti culturedin vitro. Ann. Trop. Med. Parasitol.67, 409–417 (1973).
Davey, M. W., Dalgarno, L.: Semliki Forest virus replication in culturedAedes albopictus cells: Studies on the establishment of persistence. J. gen. Virol.24, 453–463 (1974).
Enzmann, P.-J.: Induction of an interferon-like substance in persistently infectedAedes albopictus cells. Arch. ges. Virusforsch.41, 382–389 (1973).
Kitamura, S.: Thein vitro cultivation of tissues from the mosquitoes. Kobe J. Med. Sci.12, 63–70 (1966).
Macpherson, I., Stoker, M.: Polyoma transformation of hamster cell clones. An investigation of genetic factors affecting cell competence. Virology16, 147–151 (1962).
Mussgay, M.: Growth cycle of arboviruses in vertebrate and arthropod cells. Progr. med. Virol.6, 193–267 (1964).
Peleg, J.: Growth of arboviruses in monolayers from subcultured mosquito embryo cells. Virology35, 617–619 (1968).
Peleg, J.: Inapparent persistent virus infection in continuously grownAedes aegypti mosquito cells. J. gen. Virol.5, 463–471 (1969).
Peleg, J., Shahar, A.: Morphology and behaviour of culturedAedes aegypti mosquito cells. Tissue and Cell4, 55–62 (1972).
Raghow, R. S., Davey, M. W., Dalgarno, L.: The growth of Semliki Forest virus in cultured mosquito cells: Ultrastructural observations. Arch. ges. Virusforsch.43, 165–168 (1973).
Singh, K. R. P.: Cell cultures derived from larvae ofAedes albopictus (Skuse) andAedes aegypti (L.) Curr. Sci.36, 506–508 (1967).
Singh, K. R. P.: Growth of arboviruses in arthropod tissue culture. Adv. Virus Res.17, 187–206 (1972).
Stollar, V., Shenk, T. E., Stollar, B. D.: Double-stranded RNA in hamster, chick and mosquito cells infected with Sindbis virus. Virology27, 122–132 (1972).
Young, N. A., Johnson, K. M.: Antigenic variants of Venezuelan Equine Encephalitis virus: Their geographic distribution and epidemiologic significance. Amer. J. Epidemiol.89, 286–307 (1969).
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Esparza, J., Sánchez, A. Multiplication of Venezuelan Equine Encephalitis (Mucambo) virus in cultured mosquito cells. Archives of Virology 49, 273–280 (1975). https://doi.org/10.1007/BF01317545
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DOI: https://doi.org/10.1007/BF01317545