Summary
Virulent and attenuated strains of yellow fever virus were compared for their ability to grow in cultures of unstimulated leucocytes and monocytes derived from human peripheral blood, and of a macrophage-like cell line of human origin, U937. The extent of virus growth in leucocyte cultures varied depending on the strain of virus, multiplicity of infection, presence of diluted antibody in the culture medium but independently of the flavivirus immune status of the donor. The same pattern of differential growth was observed in the three types of cultures used. Although strain related variation in growth occurred within both virulent and attenuated strains, most of the attenuated strains produced higher virus yields than the virulent ones, suggesting that replication in this cell system is not related to the expression of virulence for the host. Replication in human monocytes as anin vitro marker of immunogenity for substrains of 17D vaccine virus is discussed.
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References
Boyum, A.: Isolation of mononuclear cells and granulocytes from human blood. Scand. J. Clin. Lab. Invest.21 (Suppl. 97), 77–89 (1968).
Brandt, W. E., Mc Cown, J. M., Top, F. J., Jr., Bancroft, W. H., Russell, P. K.: Effect of passage history on dengue-2 virus replication in subpopulations of human leucocytes. Infect. Immun.26, 534–541 (1979).
Daniels, C. A., Kleinerman, E. S., Shyderman, R.: Abortive and productive infections of human mononuclear phagocytes by type 1 herpes simplex virus. Am. J. Pathol.91, 119–129 (1978).
Dimmock, N. J.: Initial stages in infection with animal viruses. J. gen. Virol.59, 1–22 (1982).
Halstead, S. B., Marchette, N. J., Sung Chow, J. S., Lolekha, S.: Dengue virus replication enhancement in peripheral blood leucocytes from immune human beings. Proc. Soc. Exp. Biol. Med.151, 136–139 (1976).
Halstead, S. B., O'Rourke, E. J., Allison, A. C.: Dengue viruses and mononuclear phagocytes. II Identity of blood and tissue leucocytes supportingin vitro infection. J. Exp. Med.146, 201–217 (1977).
Halstead, S. B., Tom, M. C., Elm, J. L.:In vitro virulence marker: growth of dengue-2 virus in numan leucocyte suspension cultures. Infect. Immun.31, 102–106 (1981).
Hsiung, G. D.: Diagnostic virology. An illustrated handbook, 35–40, revised ed. New Haven: Yale University Press 1973.
Liprandi, F.: Isolation of plaque variants differing in virulence from the 17D strain of yellow fever virus. J. gen. Virol.56, 363–370 (1981).
Marker, S. C., Jahrling, P. B.: Correlation between virus-cell receptor properties of alphavirusesin vitro and virulencein vivo. Arch. Virol.62, 53–62 (1979).
Marchette, N. J., Halstead, S. B., Chow, J. S.: Replication of dengue viruses in cultures of peripheral blood leucocytes from dengue-immune rhesus monkeys. J. Infect. Dis.133, 274–282 (1976).
Mogensen, S. C.: Role of macrophages in natural resistance to virus infections. Microbiol. Rev.43, 1–26 (1979).
Monath, T. P., Brinker, K. R., Chandler, F. W., Kemp, G. E., Cropp, C. B.: Pathophysiologic correlation in a rhesus monkey model of yellow fever. Am. J. Trop. Med. Hyg.30, 431–443 (1981).
Peiris, J. S. M., Porterfield, J. S.: Antibody-mediated enhancement of flavivirus replication in macrophage-like cell lines. Nature282, 509–511 (1981).
Plaeger-Marshall, S., Wilson, L. A., Smith, J. W.: Permissiveness of rabbit monocytes and macrophages for herpes simplex virus type 1. Infect. Immun.35, 151–156 (1982).
Rodgers, B., Mims, C. A.: Interaction of influenza virus with mouse macrophages. Infect. Immun.31, 751–757 (1981).
Russell, P. K., Nisalak, A., Sukhanavachana, P., Vivona, S.: A plaque reduction neutralization for dengue virus neutralizing antibodies. J. Immunol.99, 285–290 (1967).
Schlesinger, J. J., Brandriss, M. W.: Antibody-mediated infection of macrophages and macrophage-like cell lines with 17D yellow fever virus. J. Med. Virol.8, 103–117 (1981).
Schlesinger, J. J., Brandriss, M. W.: Growth of 17D yellow fever virus in a macrophage-like cell line U937: role of FC and viral receptors in antibody mediated infection. J. Immunol.127, 659–665 (1981).
Scott, McN. R., Nisalak, A., Cheamudon, U., Seridhoranakul, S., Nimmannitya, S.: Isolation of dengue viruses from peripheral blood leucocytes of patients with hemorrhagic fever. J. Infect. Dis.141, 1–6 (1980).
Sundstrom, C., Nilsson, K.: Establishment and characterization of a human histiocytic lymphoma cell line (U 937). Int. J. Cancer17, 565–577 (1976).
Theiler, M.: The virus. In:Strode, G. (ed.), Yellow fever, 39–136. McGraw-Hill 1951.
Theiler, M., Smith, H. H.: Effect of prolonged cultivationin vitro upon pathogenicity of yellow fever virus. J. Exp. Med.65, 767–786 (1937).
Tigertt, W. D., Berge, T. O., Gochenouz, W. S., Gleiser, C. A., Eveland, W. C., Bruegge, C. V., Smetana, H. F.: Experimental yellow fever. Trans. N. Y. Acad. Sci.22, 323–333 (1960).
Wheelock, E. F., Edelman, R.: Specific role of each leucocyte type in viral infection. III 17D yellow fever virus replication and interferon production in homogenous leucocyte cultures treated with phytohemagglutinin. J. Immunol.103, 429–436 (1969).
World Health Organization: Technical reports series no. 479, WHO Expert Committee on Yellow Fever. Third Report, 1971.
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Liprandi, F., Walder, R. Replication of virulent and attenuated strains of yellow fever virus in human monocytes and macrophage-like cells (U937). Archives of Virology 76, 51–61 (1983). https://doi.org/10.1007/BF01315703
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DOI: https://doi.org/10.1007/BF01315703