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Provirus tagging as an instrument to identify oncogenes and to establish synergism between oncogenes

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Summary

Insertional mutagenesis is one of the mechanisms by which retroviruses can transform cells. Once a provirus was found in the vicinity of c-myc, with the concomitant activation of this gene, other proto-oncogenes were shown to be activated by proviral insertion in retrovirally-induced tumors. Subsequently, cloning of common proviral insertion sites led to the discovery of a series of new (putative) oncogenes. Some of these genes have been shown to fulfill key roles in growth and development. In this review I shall describe how proviruses can be used to identify proto-oncogenes, and list the loci, identified by this method. Furthermore, I shall illuminate the potential of provirus tagging by showing that it not only can mark new oncogenes, but can also be instrumental in defining sets of (onco)genes that guide a normal cell in a step-by-step fashion to its fully transformed, metastasizing, counterpart.

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Authors and Affiliations

  1. Division of Molecular Genetics, The Netherlands Cancer Institute, Amsterdam, The Netherlands

    A. Berns

  2. The Department of Biochemistry of the University of Amsterdam, Amsterdam, The Netherlands

    A. Berns

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Berns, A. Provirus tagging as an instrument to identify oncogenes and to establish synergism between oncogenes. Archives of Virology 102, 1–18 (1988). https://doi.org/10.1007/BF01315558

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  • DOI: https://doi.org/10.1007/BF01315558

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