Summary
The antigenic relationships between three similar parvoviruses, canine parvovirus type 2 (CPV), feline panleukopenia virus (FPV) and mink enteritis virus (MEV) were investigated. Antisera against all 3 viruses and monoclonal antibodies (mAb) to CPV were prepared and the viruses compared using several serological methods. When conventional sera were used in the hemagglutination-inhibition and agar gel precipitin (AGP) tests there were no differences between the CPV viral isolates studied, but antigenic differences were revealed between the CPV isolates and the FPV or MEV. Of 16 mAb produced against CPV, six reacted only with the CPV. The other 10 mAb reacted with all three parvoviruses. Additionally, an antigenic difference was detected by AGP tests between one FPV isolate and the other FPV and MEV isolates. Including both conventional sera and mAb to CPV in a single AGP test with the CPV, MEV and FPV antigens permitted the comparison of results obtained with the different antibodies. The results reported revealed antigenic differences between CPV and FPV or MEV that were most clearly defined using mAb.
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Appel, M. J. G., Scott, F. W., Carmichael, L. E.: Isolation and immunisation studies of a canine parvo-like virus from dogs with haemorrhagic enteritis. Vet. Rec.105, 156–159 (1979).
Azetaka, M., Hirasawa, T., Konishi, S., Ogata, M.: Studies on canine parvovirus isolation, experimental infection and serologic survey. Jpn. J. Vet. Sci.43, 243–255 (1981).
Binn, L. N., Marchwicki, R. H., Stephenson, E. H.: Establishment of a canine cell line: derivation, characterization and viral spectrum. Am. J. Vet. Res.41, 855–860 (1980).
Burger, D., Gorham, J. R.: Mink virus enteritis. In:Davis, J. W., Karstad, L. H., Trainer, D. O. (eds.), Infectious diseases of wild mammals, 76–84. Ames, Iowa: The Iowa State University Press 1970.
Carmichael, L. E., Joubert, J. C., Pollock, R. V. H.: Hemagglutination by canine parvovirus: serologic studies and diagnostic applications. Am. J. Vet. Res.41, 784–791 (1980).
Casals, J.: Antigenic variants of eastern equine encephalitis virus. J. Exp. Med.119, 547–564 (1964).
Farrell, R. K., Burger, D., Hartsough, G. R., Gorham, J. R.: Relationship of mink virus enteritis virus and feline panleukopenia virus: rapid onset of mink virus enteritis virus protection after feline panleukopenia virus infection. Am. J. Vet. Res.33, 2351–2352 (1972).
Fastier, L. B.: A single radial haemolysis test for measuring canine parvovirus antibody. Vet. Rec.108, 299–301 (1981).
Flower, R. L. P., Wilcox, G. E., Robinson, W. F.: Antigenic differences between canine parvovirus and feline panleukopenia virus. Vet. Rec.107, 254–256 (1980).
Gerhard, W.: Fusion of cells in suspension and outgrowth of hybrids in conditioned medium. In:Kennett, R. H., McKearn, T. J., Bechtol, K. B. (eds.), Monoclonal antibodies, 370–371. New York: Plenum 1980.
Johnson, R. H.: Isolation of a virus from a condition simulating feline panleukopaenia in a leopard. Vet. Rec.76, 1008–1012 (1964).
Johnson, R. H.: Feline panleukopaenia virus —in vitro comparison of strains with a mink enteritis virus. J. Small Anim. Pract.8, 319–324 (1967).
Johnson, R. H., Siegl, G., Gautaschi, M.: Characteristics of feline panleukopaenia virus strains enabling definitive classification as parvoviruses. Arch. ges. Virusforsch.46, 315–324 (1974).
Johnson, R. H., Spradbrow, P. B.: Isolation from dogs with severe enteritis of a parvovirus related to feline panleukopaenia virus. Aust. Vet. J.55, 151 (1979).
King, D. A., Croghan, D. L.: Immunofluorescence of feline panleukopenia virus in cell culture: determination of immunological status of felines by serum neutralization. Can. J. Comp. Med.29, 85–89 (1965).
Konishi, S., Mochizuki, M., Ogata, M.: Studies on feline panleukopenia I. Isolation and properties of virus strains. Jpn. J. Vet. Sci.37, 247–257 (1975).
Lenghaus, C., Studdert, M. J.: Relationships of canine panleukopaenia (enteritis) and myocarditis parvoviruses to feline panleukopaenia virus. Aust. Vet. J.56, 152–153 (1980).
McMaster, G. K., Traschin, I-D., Siegl, G.: Comparison of canine parvovirus with mink enteritis virus by restriction site mapping. J. Virol.38, 368–371 (1981).
Moraillon, A., Moraillon, R., Person, L. M., Parodi, A. L.: Parvovirose canine: L'ingestion d'organes de vison atteint d'entérite à virus declenche chez le chien une maladie identique à la maladie spontanée. Recl. Med. Vet.156, 539–548 (1980).
Pollock, R. V. H.: Canine parvovirus: Host-response and immunoprophylaxis. PhD Thesis, Cornell University, Ithaca, N.Y., 1981.
Shulman, M., Wilde, C. D., Köhler, G.: A better cell line for making hybridomas secreting specific antibodies. Nature276, 269–270 (1978).
Takas, B.: Electrophoresis of proteins in polyacrylamide slab gels. In:Lefkovits, I., Pernis, B. (eds.), Immunological Methods, 81–105. New York: Academic Press 1979.
Tattersall, P., Cawte, P. J., Shatkin, A. J., Ward, D. C.: Three structural polypeptides coded for by minute virus of mice, a parvovirus. J. Virol.20, 273–289 (1976).
Walker, S. T., Feilen, C. P., Sabine, M., Love, D. N., Jones, R. F.: A serological survey of canine parvovirus infection in New South Wales, Australia. Vet. Rec.106, 324–325 (1980).
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Parrish, C.R., Carmichael, L.E. & Antczak, D.F. Antigenic relationships between canine parvovirus type 2, feline panleukopenia virus and mink enteritis virus using conventional antisera and monoclonal antibodies. Archives of Virology 72, 267–278 (1982). https://doi.org/10.1007/BF01315223
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DOI: https://doi.org/10.1007/BF01315223