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Studies on glycopeptides of herpes simplex virus infected cells

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Summary

Trypsinates from HSV infected cells, radioactively labeled with glucosamine and galactose were further digested by pronase. The digests were subjected to gel filtration, and it was shown that some of the eluted material consisted of saccharides, highly devoid of residual peptide. This material eluted between linear dextran markers of 19,000 and 3000 daltons. Only slight differences between uninfected and HSV infected cells in galactose and glucosamine derived radioactivity profiles of the chromatograms were detected. High molecular weight glucosamine labeled material, present in digest from uninfected cells, was not detectable in digests from HSV infected cells.

The concanavalin A adsorbability of eluted fractions was tested. Changes in the relative adsorbability between materials from HSV and uninfected cells were present, especially for the glucosamine labeled saccharides.

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References

  1. Brennan, P. J., Steiner, S. M., Courtney, R. J., Skelly, J.: Metabolism of galactose in herpes simplex virus-infected cells. Virology69, 216–228 (1976).

    Google Scholar 

  2. Buck, C. A., Glick, M. C., Warren, L.: A comparative study of glycoproteins from the surface of control and Rous sarcoma virus transformed hamster cells. Biochem.9, 4567–4576 (1970).

    Google Scholar 

  3. Courtney, R. J., Steiner, S. M., Benyesh-Melnick, M.: Effects of 2-deoxy-D-glucose on Herpes simplex virus replication. Virology52, 447–455 (1973).

    Google Scholar 

  4. Doyle, R. J., Kan, T.-J.: Interaction between concanavalin A and heparin. FEBS letters20, 22–24 (1972).

    Google Scholar 

  5. Etchison, J. R., Holland, J. J.: Carbohydrate composition of the membrane glycoprotein of vesicular stomatitis virus grown in four mammalian cell lines. Proc. Nat. Acad. Sci. U.S.A.71, 4011–4014 (1974).

    Google Scholar 

  6. Günalp, A.: Growth and cytopathic effect of rubella virus in a line of Green Monkey kidney cells. Proc. Soc. exp. Biol. Med.118, 85–90 (1965).

    Google Scholar 

  7. Heine, J. W., Spear, P. G., Roizman, B.: Proteins specified by herpes simplex virus. VI. Viral proteins in the plasma membrane. J. Virol.9, 431–439 (1972).

    Google Scholar 

  8. Honess, R. W., Roizman, B.: Proteins specified by herpes simplex virus. XIII. Glycosylation of viral polypeptides. J. Virol.16, 1308–1326 (1975).

    Google Scholar 

  9. Keegstra, K., Sefton, B., Burke, D.: Sindbis virus glycoproteins: Effect of the host cell on the oligosaccharides. J. Virol.16, 613–620 (1975).

    Google Scholar 

  10. Keller, J. M., Spear, P. G., Roizman, B.: Proteins specified by herpes simplex virus, III. Viruses differing in their effects on the social behaviour of infected cells specify different membrane glycoproteins. Proc. Nat. Acad. Sci. U.S.A.65, 865–871 (1970).

    Google Scholar 

  11. Kobayashi, Y., Maudsley, D. V.: Practical aspects of liquid scintillation counting. In:Glick, D. (ed.), Methods of biochemical analysis,17, 55–133. New York: John Wiley and Sons 1969.

    Google Scholar 

  12. Ogata, S.-I., Muramatsu, T., Kobata, B.: Fractionation of glycopeptides by affinity column chromatography on concanavalin A-Sepharose. J. Biochem. (Tokyo)78, 687–696 (1975).

    Google Scholar 

  13. Savage, T., Roizman, B., Heine, J. W.: Immunological specificity of the glycoproteins of herpes simplex virus subtypes 1 and 2. J. gen. Virol.17, 31–48 (1972).

    Google Scholar 

  14. Schmid, P.: Extraction and purification of lipids: II. Why is chloroform-methanol such a good lipid solvent? Physiol. Chem. Physic5, 141–150 (1973).

    Google Scholar 

  15. So, L. L., Goldstein, I. J.: Protein-carbohydrate interaction. XIII. The interaction of concanavalin A with α-mannans from a variety of microorganisms. J. biol. Chem.243, 2003–2007 (1968).

    Google Scholar 

  16. Spear, P. G., Keller, J. M., Roizman, B.: Proteins specified by herpes simplex virus. II. Viral glycoproteins associated with cellular membranes. J. Virol.5, 123–131 (1970).

    Google Scholar 

  17. Spear, P. G., Roizman, B.: Proteins specified by herpes simplex virus. V. Purification and structural proteins of the herpesvirion. J. Virol.9, 143–159 (1972).

    Google Scholar 

  18. Spiro, R. G.: The carbohydrate units of thyroglobulin. J. biol. Chem.240, 1603–1610 (1965).

    Google Scholar 

  19. Spiro, R. G.: Glycoproteins. Adv. Protein Chem.27, 349–467 (1973).

    Google Scholar 

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Authors and Affiliations

  1. Department of Virology, Institute of Medical Microbiology, University of Göteborg, Göteborg, Sweden

    S. Olofsson & J. Blomberg

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  1. S. Olofsson
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  2. J. Blomberg
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Olofsson, S., Blomberg, J. Studies on glycopeptides of herpes simplex virus infected cells. Archives of Virology 55, 293–304 (1977). https://doi.org/10.1007/BF01315051

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  • DOI: https://doi.org/10.1007/BF01315051

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