Summary
Intracutaneous immunization of BALB/c mice with inactivated Semliki Forest virus mixed with the cationic, surface active lipid dimethyl dioctadecyl ammoniumbromide produced a strong enhancement of delayed type hypersensitivity without detectable antibodies in serum. Furthermore these mice were more protected against intraperitoneal challenge than normal immunized mice. This protection might be explained by an enhancement of cellular immunity against Semliki Forest virus. Dimethyl dioctadecyl ammoniumbromide by itself has no effect on footpad swelling, mortality and mean survival time.
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References
Acheson, N. H., Tamm, I.: Replication of Semliki Forest virus: An electron microscopic study. Virology32, 128–143 (1967).
Ashwell, G., Morell, A. G.: The role of surface carbohydrates in the hepatic recognition and transport of circulating glycoproteins. Adv. Enzym.41, 99–128 (1974).
Bradish, C. J., Allner, K., Maber, H. B.: The virulence of original and derived strains of Semliki Forest virus for mice, guinea-pigs and rabbits. J. gen. Virol.12, 141–160 (1971).
Dailey, M. O., Hunter, R.: The role of lipid in the induction of hapten-specific delayed hypersensitivity and contact sensitivity. J. Immunol.112, 1526–1534 (1974).
Dailey, M. O., Post, W., Hunter, R. L.: Induction of cell-mediated immunity to chemically modified antigens in guinea-pigs. II. The interaction between lipid-conjugated antigens, macrophages and T-lymphocytes. J. Immunol.118, 963–970 (1977).
Fleming, P.: Age-dependent and strain-related differences of virulence of Semliki Forest virus in mice. J. gen. Virol.37, 93–105 (1977).
Garoff, H., Simons, K., Renkonen, O.: Isolation and characterization of the membrane proteins of Semliki Forest virus. Virology61, 493–504 (1974).
Kennedy, S. I. T.: The effect of enzymes on structural and biological properties of Semliki Forest virus. J. gen. Virol.23, 129–143 (1974).
Koster, F. T., McGregor, D. D., Mackaness, G. B.: The mediator of cellular immunity. II. Migration of immunologically committed lymphocytes into inflammatory exudates. J. exp. Med.133, 400–409 (1971).
Kraaijeveld, C. A., Harmsen, M., Khader Boutahar-Trouw, B.: Delayed-type hypersensitivity against Semliki Forest virus in mice. Infect. Immun.23, 219–223 (1979).
Kraaijeveld, C. A., Harmsen, M., Khader Boutahar-Trouw, B.: Cellular immunity against Semliki Forest virus in mice. Infect. Immun.23, 213–218 (1979).
Lagrange, P. B., Mackaness, G. B., Miller, T. E.: Influence of dose and route of antigen injection on the immunological induction of T cells. J. exp. Med.139, 528–542 (1974).
Laine, R., Kettunen, M., Gahmberg, C. G., Kääriänen, L., Renkonen, O.: Fatty chains of different lipid classes of Semliki Forest virus and host cell membranes. J. Virol.10, 433–438 (1972).
Luukkonen, A., Gahmberg, C. G., Renkonen, O.: Surface labeling of Semliki Forest virus glycoproteins using galactose oxidase. Virology76, 55–59 (1977).
Neurath, A. R., Hashimoto, N., Prince, A. M.: Sialyl residues in hepatitis B antigen: their role in determining the life span of the antigen in serum and in eliciting an immunological response. J. gen. Virol.27, 81–91 (1975).
Pusztai, R., Gould, E. A., Smith, H.: Infection patterns in mice of an avirulent and virulent strain of Semliki Forest virus. Brit. J. exp. Pathol.52, 669–677 (1971).
Renkonen, O., Kääriänen, L., Simons, K., Gahmberg, C. G.: The lipid class composition of Semliki Forest virus and of plasma membranes of the host cells. Virology46, 318–326 (1971).
Sela, M.: The antigens, Vol. II. New York: Academic Press 1974.
Silberberg-Sinakin, I., Baer, R. L., Thorbecke, G. J.: Langerhans cells. A review of their nature with emphasis on their immunologic functions. Progr. Allergy24, 268–294 (1978).
Snippe, H., Belder, M., Willers, J. M. N.: Dimethyl dioctadecyl ammonium-bromide as adjuvant for delayed hypersensitivity in mice. Immunology33, 931 to 936 (1977).
Turk, J. L., Poulter, L. W.: Selective depletion of lymphoid tissue by cyclophosphamide. Clin. exp. Immunol.10, 285–296 (1972).
Utermann, G., Simons, K.: Studies on the amphipathic nature of the membrane proteins in Semliki Forest virus. J. mol. Biol.85, 569–587 (1974).
Van Dijk, H., Versteeg, H., Hennink, H. J.: A semi-electronic device for measuring delayed hypersensitivity in the mouse by footpad swelling. J. immunol. Meth.12, 261–265 (1976).
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Kraaijeveld, C.A., Snippe, H., Harmsen, M. et al. Dimethyl dioctadecyl ammoniumbromide as an adjuvant for delayed type hypersensitivity and cellular immunity against Semliki Forest virus in mice. Archives of Virology 65, 211–217 (1980). https://doi.org/10.1007/BF01314537
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DOI: https://doi.org/10.1007/BF01314537