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Advances in the use of nucleic acid probes in diagnosis of viral diseases of man

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Summary

A variety of methods are now available for the preparation and labelling of viral nucleic acids for use as probes in diagnostic virology. Some of these are assessed including the use of synthetic oligonucleotides in place of molecularly cloned nucleic acids, and alternatives to labelling with radioactive isotopes such as biotin, enzymes and fluorochromes. Dot blot, sandwich, indirect sandwich and in situ hybridization are covered, and examples given of the current use of nucleic acid probes in detection of human viral infections. The potential and limitations of nucleic acid hybridization are discussed in the light of these new methods.

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References

  1. Aksamit AJ, Mourrain P, Sever JL, Major EO (1985) Progressive multifocal leukoencephalopathy investigation of three cases using in situ hybridization with JC virus biotinylated DNA probe. Ann Neurol 18: 490–496

    Google Scholar 

  2. Al-Nakib W, Stanway G, Forsyth M, Hughes PJ, Almond JW, Tyrrell DAJ (1986) Detection of human rhinoviruses and their molecular relationship using cDNA probes. J Med Virol 20: 289–296

    Google Scholar 

  3. Ambinder RF, Charache P, Staal S, Wright P, Forman M, Hayward SD, Hayward GS (1986) The vector homology problem in diagnostic nucleic acid hybridization of clinical specimens. J Clin Microbiol 24: 16–20

    Google Scholar 

  4. Ambinder RF, Wingard JR, Burns WH, Hayward SD, Sarai R, Perry HR, Santos GW, Hayward GS (1985) Detection of Epstein Barr virus DNA in mouth washes by hybridization. J Clin Microbiol 21: 353–356

    Google Scholar 

  5. Anand A, Gray ES, Brown T, Clewley JP, Cohen BJ (1987) Human parvovirus infection in pregnancy and hydrops fetalis. N Engl J Med 316: 183–186

    Google Scholar 

  6. Anderson MJ, Jones SE, Minson AC (1985) Diagnosis of human parvovirus infection by dot-blot hybridization using cloned viral DNA. J Med Virol 15: 163–172

    Google Scholar 

  7. Bauman JFG, Wiegant J, van Duijn P (1981) Cytochemical hybridization with fluorochrome-labelled RNA. I. Development of a method using nucleic acid bound to agarose beads as a model. J Histochem Cytochem 29: 227–237

    Google Scholar 

  8. Bauman JG, Wiegant J, van Duijn P (1981) Cytochemical hybridization with fluorochrome-labelled RNA. II. Applications. J Histochem Cytochem 29: 227–237

    Google Scholar 

  9. Beckmann AM, Myerson D, Daling JR, Kiviat N, Fenoglio CM, McDougall JK (1985) Detection and localization of human papilloma virus DNA in human genital condylomas by in situ hybridization with biotinylated probes. J Med Virol 16: 265–273

    Google Scholar 

  10. Bornkamm GW, Desgranges C, Gissmann L (1983) Nucleic acid hybridization for the detection of viral genomes. Curr Top Microbiol Immunol 104: 287–298

    Google Scholar 

  11. Brechot C, Hadchouel M, Scotts J, Degos F, Charnay P, Trepo C, Tiollais P (1981) Detection of hepatitis B DNA in liver and serum: a direct appraisal of the chronic carrier state. Lancet 2: 765–768

    Google Scholar 

  12. Bresser J, Doering J, Gillespie D (1983) Quick-blot—selective messenger-RNA or DNA immobilization from whole cells. DNA 2: 243–254

    Google Scholar 

  13. Chowdhury SI, Hammerschmidt W, Ludwig H, Thein P, Buhk H-J (1986) Rapid method for the identification and screening of herpesvirus by DNA finger printing combined with blot hybridization. J Virol Methods 14: 285–291

    Google Scholar 

  14. Conner BJ, Reyes AA, Murin C, Itakura K, Teplitz RL, Wallace RB (1983) Detection of sickle cell βS-globulin allele by hybridization with synthetic oligonucleotides. Proc Natl Acad Sci USA 80: 278–282

    Google Scholar 

  15. Danos O, Katinka M, Yaniv M (1980) Molecular cloning, refined physical map and heterogeneity of methylation sites of papilloma virus type 1 a DNA. Eur J Biochem 109: 475–461

    Google Scholar 

  16. de Villiers E-M, Gissmann L, zur Hausen H (1981) Molecular cloning of viral DNA from human genital warts. J Virol 40: 932–935

    Google Scholar 

  17. Diaz-Mitroma F, Preiksaitis JK, Leung WC, Tyrrell DLJ (1987) DNA dot hybridization to detect Epstein-Barr virus in throat washings. J Infect Dis 155: 297–303

    Google Scholar 

  18. Diegutis PS, Keirnan E, Burnett L, Nightingale BN, Cossart YE (1986) False-positive results with hepatitis B DNA dot-hybridization in hepatitis B surface antigennegative specimes. J Clin Microbiol 23: 797–799

    Google Scholar 

  19. Durst M, Kleinheinz A, Hotz M, Gissmann L (1985) The physical state of human papillomavirus type 16 DNA in benign and malignant genital tumours. J Gen Virol 66: 1515–1522

    Google Scholar 

  20. Fleckenstein B, Muller I, Collins J (1982) Cloning of the complete human cytomegalovirus in cosmids. Gene 18: 39–46

    Google Scholar 

  21. Flewett TH (1985) Rapid diagnosis of virus diseases. Br Med Bull 41: 315–321

    Google Scholar 

  22. Forster AC, McInnes JL, Skingle DC, Symons RH (1985) Non-radioactive hybridization probes prepared by the chemical labelling of DNA and RNA with a novel reagent, photobiotin. Nucleic Acids Res 13: 745–761

    Google Scholar 

  23. Galloway DA, Swain M (1980) Cloning of Herpes simplex virus type 2 DNA fragments in a plasmid vector. Gene 11: 253–257

    Google Scholar 

  24. Gendelman HE, Moench TR, Narayan O, Graffin DE, Clements JE (1985) A double labeling technique for performing immunocytochemistry and in situ hybridization in virus infected cell cultures and tissues. J Virol Methods 11: 93–103

    Google Scholar 

  25. Goldin AL, Sandri-Goldin RM, Levine M, Glorioso JC (1981) Cloning of herpes simplex type 1 sequences representing the whole genome. J Virol 38: 50–58

    Google Scholar 

  26. Gomes SA, Sascimin JP, Siqueira MM, Krawczuk MM, Pereira HG, Russell WC (1985) In situ hybridization with biotinylated DNA probes: a rapid diagnostic test for adenovirus upper respiratory infection. J Virol Methods 12: 105–110

    Google Scholar 

  27. Habermehl K-O (ed) (1985) Rapid methods and automation in microbiology and immunology. Springer Verlag, Berlin Heidelberg New York Tokyo

    Google Scholar 

  28. Henchal EA, Narupiti S, Feighny R, Padmanabhan P, Vakharia V (1987) Detection of dengue virus RNA using nucleic acid hybridization. J Virol Methods 15: 187–200

    Google Scholar 

  29. Hernandez-Jairegui P, Eriksson A, Perez RT, Pettersson U, Moreno-Lopez J (1987) Human papillomavirus type 13 DNA in focal epithelial hyperplasia among Mexicans. Arch Virol 93: 131–137

    Google Scholar 

  30. Hyypia T, Stalhandske P, Vainionpaa R, Pettersson U (1984) Detection of Enteroviruses by spot hybridization. J Clin Microbiol 19: 436–438

    Google Scholar 

  31. Khan AM, Wright PJ (1987) Detection of flavivirus RNA in infected cells using photobiotin-labelled hybridization probes. J Virol Methods 15: 121–130

    Google Scholar 

  32. Kulski JK, Norval M (1985) Nucleic acid probes in diagnosis of viral diseases of man. Arch Virol 83: 3–15

    Google Scholar 

  33. Langdale JA, Malcolm ADB (1985) A rapid method of gene detection using DNA bound to Sephacryl. Gene 36: 201–210

    Google Scholar 

  34. Lehtomaki K, Julkunen I, Sandelin K, Salonen J, Virtanen M, Ranki M, Hovi T (1986) Rapid diagnosis of respiratory adenovirus infection in young adult men. J Clin Microbiol 24: 108–111

    Google Scholar 

  35. Lin HJ, Wu P-C, Lai C-L (1987) An oligonucleotide probe for the detection of hepatitis B virus DNA in serum. J Virol Methods 15: 139–149

    Google Scholar 

  36. Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory, New York

    Google Scholar 

  37. McCance DJ (1986) Human papillomaviruses and cancer. Biochem Biophys Acta 823: 195–205

    Google Scholar 

  38. McKeating JA, Al-Nakib W, Greenaway PJ, Griffith PD (1985) Detection of cytomegalovirus by DNA-DNA hybridization employing probes labelled with32-phosphorus or biotin. J Virol Methods 11: 207–216

    Google Scholar 

  39. Meanwell CA, Cox MF, Blackledge G, Maitland N (1987) HPV 16 DNA in normal and malignant cervical epithelium: implication for the aetiology and behaviour of cervical neoplasia. Lancet 1: 703–707

    Google Scholar 

  40. Meinkoth J, Wahl G (1984) Hybridization of nucleic acids immobilized on solid supports. Anal Biochem 138: 267–284

    Google Scholar 

  41. Messing J (1983) New M 13 vectors for cloning. In: Wu R, Grossman L, Moldave K (eds) Methods in enzymology, vol 101. Academic Press, New York, pp 20–78

    Google Scholar 

  42. Mitchell R, Ambros P, Gosden JR, Morten JE, Porteous DJ (1986) Gene mapping and physical arrangements of human chromation in transformed, hybrid cells: fluorescent and autoradiographic in situ hybridization compared. Somatic Cell Mol Genet 12: 313–324

    Google Scholar 

  43. Moench TR, Gendelman HE, Clements JE, Narayan O, Griffin DE (1985) Efficiency of in situ hybridization as a function of probe size and fixation method. J Virol Methods 11: 119–130

    Google Scholar 

  44. Monroe JE, Andrews C, Sullivan JL, Mulder C (1987) Use of cytoplasmic dot-blot hybridization to detect human immunodeficiency virus RNA sequences in cultures of peripheral blood. J Infect Dis 155: 320–322

    Google Scholar 

  45. Morace G, van der Helm K, Jilg W, Deinhardt F (1985) Detection of hepatitis B viral DNA in serum by a rapid-filtration hybridization assay. J Virol Methods 12: 235–242

    Google Scholar 

  46. Parkkinen S, Mantyjarvi R, Syrjanen K, Ranki M (1986) Detection of human papillomavirus DNA by the nucleic acid sandwich hybridization method from cervical scrapings. J Med Virol 20: 279–288

    Google Scholar 

  47. Pettersson U, Hyypia T (1985) Nucleic acid hybridization — an alternative tool in diagnostic microbiology. Immunol Today 6: 268–272

    Google Scholar 

  48. Ranki M, Virtanen M, Palva A, Laaksonen M, Pettersson R, Kaariainen L, Halonen P, Soderlund H (983) Nucleic acid sandwich hybridization in adenovirus diagnosis. Curr Top Microbiol Immunol 104: 307–318

  49. Renz M, Kurz C (1984) A colorimetric method for DNA hybridization. Nucleic Acids Res 12: 3435–3444

    Google Scholar 

  50. Richman DD, Cleveland PH, Redfield DC, Oxman MN, Wahl GM (1984) Rapid viral diagnosis. J Infect Dis 149; 298–310

    Google Scholar 

  51. Rotbard HA, Levin MJ, Villarreal LP, Tracy SM, Semler BL, Wimmer E (1985) Factors affecting the detection of enteroviruses in cerebrospinal fluid with Coxsackie B3 and poliovirus 1 cDNA probes. J Clin Microbiol 22: 220–224

    Google Scholar 

  52. Schuster V, Matz B, Wiegand H, Traub B, Kampa D, Neumann-Haefelin D (1986) Detection of human cytomegalovirus in urine by DNA-DNA and RNA-DNA hybridizations. J Infect Dis 154: 309–314

    Google Scholar 

  53. Spector SA, Rua JA, Spector DH, McMillan R (1984) Detection of human cytomegalovirus in clinical specimens by DNA-DNA hybridization. J Infect Dis 150: 121–126

    Google Scholar 

  54. Stalhandske P, Hyypia T, Gadler H, Halonen P, Pettersson U (1983) The use of molecular hybridization for demonstration of adenovirus in human stools. Curr Top Microbiol Immunol 104: 229–306

    Google Scholar 

  55. Stenlund A, Perricaudet M, Tiollais P, Pettersson U (1980) Construction of restriction enzyme fragment libraries containing DNA from human adenovirus types 2 and 5. Gene 10: 47–52

    Google Scholar 

  56. Straus SE, Owens J, Ruyechan WT, Takiff HE, Casey TA, vande Woude GF, Hay J (1982) Molecular cloning and physical mapping of varicella zoster virus DNA. Proc Natl Acad Sci USA 79: 993–997

    Google Scholar 

  57. Thomas HC, Karayian P, Fowler MJ, Monjardi I (1985) Clinical uses of hepatitis B virus-DNA assays. J Virol Methods 10: 291–294

    Google Scholar 

  58. Tourtellotte WW, Verity AN, Schmid P, Martinez S, Shapshak P (1987) Covalent binding of formalin fixed paraffin embedded brain tissue sections to glass slides suitable for in situ hybridization. J Virol Methods 15: 87–100

    Google Scholar 

  59. Unger ER, Budgeon LR, Myerson D, Brigati DJ (1986) Viral diagnosis by in situ hybridization. Am J Surg Pathol 10: 1–8

    Google Scholar 

  60. Virtanen M, Palva A, Laaksonen M, Halonen P, Soderlund H, Ranki M (1983) Novel test for rapid viral diagnosis: detection of adenovirus in nasopharyngeal mucus aspirates by means of nucleic-acid sandwich hybridization. Lancet 1: 381–383

    Google Scholar 

  61. Virtanen M, Syvanen A-C, Oram J, Soderlund H, Ranki M (1984) Cytomegalovirus in urine: detection of viral DNA by sandwich hybridization. J Clin Microbiol 20: 1083–1088

    Google Scholar 

  62. Viscidi RP, Connelly CJ, Yolken RH (1986) Novel chemical method for the preparation of nucleic acid for non-isotopic hybridization. J Clin Microbiol 23: 311–317

    Google Scholar 

  63. Vonsover A, Leventon-Kriss S, Langer A, Smetana Z, Zaizov R, Potaznick D, Cohen IJ, Gotlieb-Stematsky T (1987) Detection of varicella zoster virus in lymphocytes by DNA hybridization. J Med Virol 21: 57–66

    Google Scholar 

  64. Wickenden C, Steele A, Malcolm AD, Coleman DV (1985) Screening for wart virus infection in normal and abnormal cervices by DNA hybridization of cervical scrapes. Lancet 1: 65–67

    Google Scholar 

  65. Wolf H, Leser U, Haus M, Gu SY, Pathmanathan R (1986) Sandwich nucleic acid hybridization: a method with a universally usable labelled probe for various specific tests. J Virol Methods 13: 1–8

    Google Scholar 

  66. Yousaf SI, Carroll AR, Clarke BE (1984) A new and improved method for 3′-end labelling DNA using [α-32P] dd ATP. Gene 27: 309–313

    Google Scholar 

  67. Ziegler T, Hukkanen V, Artstila P, Auvinen P, Jalava A, Hyypia T (1985) Typing of herpes simplex virus isolates with monoclonal antibodies and by nucleic acid hybridization. J Virol Methods 12: 169–177

    Google Scholar 

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Authors and Affiliations

  1. Department of Bacteriology, University of Edinburgh Medical School, Edinburgh

    Mary Norval

  2. Department of Veterinary Pathology, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh

    R. W. Bingham

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  1. Mary Norval
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Norval, M., Bingham, R.W. Advances in the use of nucleic acid probes in diagnosis of viral diseases of man. Archives of Virology 97, 151–165 (1987). https://doi.org/10.1007/BF01314418

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  • DOI: https://doi.org/10.1007/BF01314418

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