Skip to main content
SpringerLink
Log in

The effect of cordycepin on the multiplication of Semliki Forest virus and on polyadenylation of viral RNA

  • Original Papers
  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

Cordycepin (3′-deoxyadenosine), at a concentration of 20 µg/ml, has a marked effect on Semliki Forest virus multiplication. The appearance of plaque forming units is delayed by about 2 hours and the yield greatly reduced. The incorporation of [3H] uridine into intracellular viral RNAs reaches less than 50 per cent of controls. However, no specific effect on poly (A) synthesis could be detected. The binding efficiency of viral RNAs on nitrocellulose membranes and poly (U) sepharose is not affected by cordycepin. The average poly (A) length of total intracellular viral RNA was calculated on the basis of the ratio of the radioactivity of adenosine-monophosphate: adenosine and found to be about 35 nucleotides in treated and untreated cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Penman, S., Rosbash, M., Penman, M.: Messenger and heterogeneous nuclear RNA in HeLa cells: differential inhibition by cordycepin. Proc. Nat. Acad. Sci. U.S.A.67, 1878–1885 (1970).

    Google Scholar 

  2. Philipson, L., Wall, R., Glickman, G., Darnell, J.: Addition of polyadenylate sequences to virusspecific RNA during Adenovirus replication. Proc. Nat. Acad. Sci. U.S.A.68, 2806–2809 (1971).

    Google Scholar 

  3. Huez, G., Marbaix, G., Hubert, E., Leclercq, M., Nudel, U., Soreq, H., Salomon, R., Lebleu, B., Revel, M., Littauer, U. Z.: Role of the polyadenylate segment in the translation of globin messenger RNA in Xenopus oocytes. Proc. Nat. Acad. Sci. U.S.A.71, 3143–3146 (1974).

    Google Scholar 

  4. Sheiness, D., Puckett, L., Darnell, J. E.: Possible relationship of poly(A) shortening to mRNA turnover. Proc. Nat. Acad. Sci. U.S.A.72, 1077–1081 (1975).

    Google Scholar 

  5. Stoltzfus, C. M., Shatkin, A. J., Banerjee, A. K.: Absence of polyadenylic acid from reovirus messenger ribonucleic acid. J. biol. Chem.248, 7993–7998 (1973).

    Google Scholar 

  6. Spector, D. H., Baltimore, D.: Requirement of 3′-terminal poly (adenylic acid) for the infectivity of Poliovirus RNA. Proc. Nat. Acad. Sci. U.S.A.71, 2983–2987 (1974).

    Google Scholar 

  7. Goldstein, N. O., Pardoe, I. U., Burness, A. T. H.: Requirement of an Adenylic Acid-Rich Segment for the Infectivity of Encephalomyocarditis Virus RNA. J. gen. Virol.31, 271–276 (1976).

    Google Scholar 

  8. Hruby, D. E., Roberts, W. K.: Encephalomyocarditis Virus RNA: Variations in Polyadenylic Acid Content and Biological Activity J. Virol.19, 325–330 (1976).

    Google Scholar 

  9. Nair, C. N., Owens, M. J.: Preliminary observations pertaining to polyadenylation of rhinovirus RNA. J. Virol.13, 535–537 (1974).

    Google Scholar 

  10. Mahy, B. W. J., Cox, N. J., Armstrong, S. J., Barry, R. D.: Multiplication of influenza virus in the presence of cordycepin, an inhibitor of cellular RNA synthesis. Nature (London) N. Biol.243, 172–174 (1973).

    Google Scholar 

  11. Rutherford, R. B., Hare, J. D.: The synthesis and turnover of virus-specific polyadenylated RNA in Polyoma-infected cells. Biochem. biophys. Res. Comm.62, 789–797 (1975).

    Google Scholar 

  12. Nevins, J. R., Joklik, W. K.: Poly(A) sequences of Vaccinia virus messenger RNA: Nature, mode of addition and function during translationin vitro andin vivo. Virology63, 1–14 (1975).

    Google Scholar 

  13. van Oortmerssen, E. A. C., Regensburg, B. A., Tasseron-de Jong, J. G., Bosch, L.: Differential Inhibition of thein vivo Synthesis of Adenovirus Type 5-Specific Ribonucleic Acids by Cordycepin. Virology65, 238–247 (1975).

    Google Scholar 

  14. Ehrenfeld, E.: Polyadenylation of Vesicular Stomatitis Virus mRNA. J. Virol.13, 1055–1060 (1974).

    Google Scholar 

  15. Murphy, M. F., Lazzarini, R. A.: Synthesis of Viral mRNA and Polyadenylate by a Ribonucleoprotein Complex from Extracts of VSV-Infected Cells. Cell3, 77–84 (1974).

    Google Scholar 

  16. Weiss, S. R., Bratt, M. A.: Effect of Cordycepin (3′-Deoxyadenosine) on Virus-Specific RNA Species Synthesized in Newcastle Disease Virus-Infected Cells. J. Virol.16, 1575–1583 (1975).

    Google Scholar 

  17. Clegg, J. C. S., Kennedy, S. I. T.: Polyadenylic acid sequences in the virus RNA species of cells infected with Semliki Forest virus. J. gen. Virol.22, 331–345 (1974).

    Google Scholar 

  18. Brawerman, G., Mendecki, J., Lee, S. Y.: A procedure for the isolation of mammalian messenger ribonucleic acid. Biochem. (U.S.A.)11, 637–641 (1972).

    Google Scholar 

  19. Perry, R. P., la Torre, J., Kelly, D. E., Greenberg, J. R.: On the lability of poly(A) sequences during extraction of messenger RNA from polyribosomes. Biochim. biophys. Acta262, 220–226 (1972).

    Google Scholar 

  20. Koblet, H., Kohler, U., Wyler, R.: Optimization of the interferon assay using inhibition of Semliki Forest virus ribonucleic acid synthesis. Appl. Microbiol.24, 323–327 (1972).

    Google Scholar 

  21. Koblet, H., Kohler, U., Wyler, R.: Stimulation of ribo-nucleic acid synthesis in chick-embryo fibroblasts by exogenous adenosine 3′:5′-monophosphate. Europ. J. Biochem.37, 134–142 (1973).

    Google Scholar 

  22. Lindenmann, J., Gifford, G. E.: Studies on vaccinia virus plaque formation and its inhibition by interferon. Virology19, 302–309 (1963).

    Google Scholar 

  23. Eaton, B. T., Faulkner, P.: Heterogeneity in the poly(A) content of the genome of Sindbis virus. Virology50, 865–873 (1972).

    Google Scholar 

  24. Baltimore, D., Girard, M.: An intermediate in the synthesis of poliovirus RNA. Proc. Nat. Acad. Sci. U.S.A.56, 741–748 (1966).

    Google Scholar 

  25. Adesnik, M., Salditt, M., Thomas, W., Darnell, J. E.: Evidence that all messenger RNA molecules (except histone messenger RNA) contain poly(A) sequences and that the poly(A) has a nuclear function. J. mol. Biol.71, 21–30 (1972).

    Google Scholar 

  26. Keith, J., Gleason, M., Fraenkel-Conrat, H.: Characterization of the end groups of RNA of Rous Sarcoma virus. Proc. Nat. Acad. Sci. U.S.A.71, 4371–4375 (1974).

    Google Scholar 

  27. Bruton, C. J., Kennedy, S. I. T.: Semliki Forest virus intracellular RNA: Properties of the multi-stranded RNA species and kinetics of positive and negative strand synthesis. J. gen. Virol.28, 111–127 (1975).

    Google Scholar 

  28. Glazer, R. I.: The action of cordycepin on nascent nuclear RNA and poly(A) synthesis in regenerating liver. Biochim. biophys. Acta418, 160–166 (1976).

    Google Scholar 

  29. Blatti, S. P., Ingles, C. J., Lindell, T. J., Morris, P. W., Weaver, R. F., Weinberg, F., Rutter, W. J.: Structure and regulatory properties of eukaryotic RNA polymerase. Cold Spring Harbor Symposia on quantitative Biology35, 649–657 (1970).

    Google Scholar 

  30. Fouquet, H., Wick, R., Bohme, R., Sauer, H. W., Scheller, K.: Effects of Cordycepin on RNA Synthesis in Physarum polycephalum. Arch. Biochem. Biophys.168, 273–280 (1975).

    Google Scholar 

  31. Maale, G., Stein, G., Mans, R.: Effects of Cordycepin and cordycepintriphosphate on polyadenylic and ribonucleic acid-synthesizing enzymes from eukaryotes. Nature255, 80–82 (1975).

    Google Scholar 

  32. Colby, D. S., Finnerty, V., Lucas-Lenard, J.: Fate of mRNA of L-cells infected with Mengovirus. J. Virol.13, 858–869 (1974).

    Google Scholar 

  33. la Torre, J., Perry, R. P.: The relationship between polyadenylated heterogeneous nuclear RNA and messenger RNA: Studies with actinomycin D and cordycepin. Biochim. biophys. Acta335, 93–101 (1973).

    Google Scholar 

  34. Follett, E. A. C., Pringle, C. R., Pennington, T. H.: Virus development in enucleate cells: Echovirus, Poliovirus, Pseudorabies virus, Reovirus, Respiratory Syncytial virus and Semliki Forest virus. J. gen. Virol.26, 183–196 (1975).

    Google Scholar 

  35. Nair, C. N., Panicali, D. L.: Polyadenylate Sequences of Human Rhinovirus and Poliovirus RNA and Cordycepin Sensitivity of Virus Replication. J. Virol.20, 170–176 (1976).

    Google Scholar 

  36. Sawicki, D. L., Gomatos, P. J.: Replication of Semliki Forest Virus: Polyadenylate in Plus-Strand RNA and Polyuridylate in Minus-Strand RNA. J. Virol.20, 446–464 (1976).

    Google Scholar 

  37. Spector, D. H., Baltimore, D.: Polyadenylic Acid on Poliovirus RNA IV. Poly(U) in Replicative Intermediate and Double-Stranded RNA. Virology67, 498–505 (1975).

    Google Scholar 

  38. Deborde, D. C., Leibowitz, R. D.: Polyadenylic Acid Size and Position found in Sindbis Virus Genome and mRNA Species. Virology72, 80–88 (1976).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Virologie der Universität Zürich, Zürich, Switzerland

    R. Wittek, A. Menna & R. Wyler

  2. Institut für Hygiene und medizinische Mikrobiologie der Universität Bern, Bern, Switzerland

    H. Koblet

Authors
  1. R. Wittek
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. Koblet
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. Menna
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. R. Wyler
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

With 2 Figures

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wittek, R., Koblet, H., Menna, A. et al. The effect of cordycepin on the multiplication of Semliki Forest virus and on polyadenylation of viral RNA. Archives of Virology 54, 95–106 (1977). https://doi.org/10.1007/BF01314382

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01314382

Keywords

Search

Navigation