Summary
An improved rapid focus reduction neutralization test for Japanese encephalitis (JE) virus in microtiter system was developed. 96-well tissue culture plates were used for preparation of cell monolayers and PAP (peroxidase-antiperoxidase) staining technique was used for visualization of foci of infected cells. As the test has many advantages over other methods, it might be applied widely for rapid diagnosis and seroepidemiological studies.
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References
Fukunaga, T., Rojanasuphot, S., Wungkorbkiat, S., Thammanichanon, A., Kan, T., Otsu, K., Tuchinda, P., Jatanasen, S., Chiowanich, P.: Japanese encephalitis vaccination in Thailand. Biken J.17, 21–31 (1974).
Graham, R. C., Jr., Karnovsky, M. J.: The early stages of adsorption of injected horseradish peroxidase in the proximal tubules of monkey kidney: ultrastructural cytochemistry by a new technique. J. Histochem. Cytochem.14, 291–302 (1966).
Gunakasem, P., Chantrasri, C., Simasathien, P., Chaiyanun, S., Jatanasen, S., Pariyanonth, A.: Surveillance of Japanese encephalitis cases in Thailand. Southeast Asian J. Trop. Med. Pub. Hlth.12, 333–337 (1981).
Igarashi, A.: Isolation of a Singh'sAedes albopictus cell clone sensitive to dengue and chikungunya viruses. J. gen. Virol.40, 531–544 (1978).
Igarashi, A., Ogata, T., Fujita, N., Fukunaga, T., Mori, A., Uzuka, Y., Supawadee, J., Chiowanich, P., Peerakome, S., Leechanachai, P., Charoensook, O., Chanyasanha, C.: Flavivirus infections in Chiang Mai area, Thailand, in 1982. Southeast Asian J. Trop. Med. Pub. Hlth.14, 470–480 (1983).
Juang, R. F., Okuno, Y., Fukunaga, T., Tadano, M., Fukai, K., Baba, K., Tsuda, N., Yamada, A., Yabuuchi, H.: Neutralizing antibody responses to Japanese encephalitis vaccine in children. Biken J.26, 25–34 (1983).
Kalimuddin, Md., Narayan, K. G., Choudhary, P.: Possible relation of seropositivity to Japanese encephalitis in pigs with the 1978 epidemic of human encephalitis in Bihar. Trans. R. Soc. Trop. Med. Hyg.76, 639–640 (1982).
Okuno, Y., Fukunaga, T., Srisupaluck, P., Kasemsarn, P., Dharakul, C., Sangkawibha, N.: Serological and virological studies on patients with dengue hemorrhagic fever (DHF) in Chanthaburi Province, Thailand. I. Serological studies on paired sera from DHF patients by neutralization (N), hemagglutination inhibition (HI) and staining tests. Biken J.23, 113–121 (1980).
Okuno, Y., Fukunaga, T., Tadano, M., Fukai, K., Ikeda, T., Sekii, K., Ariyoshi, H.: Serological studies on volunteers inoculated experimentally with dengue virus strain in 1943. Biken J.26, 161–163 (1983).
Okuno, Y., Igarashi, A., Fukai, K.: Neutralization tests for dengue and Japanese encephalitis viruses by the focus reduction method using peroxidase-anti-peroxidase staining. Biken J.21, 137–147 (1978).
Okuno, Y., Yamanishi, K., Lwin, S., Takahashi, M.: Micro-neutralization test for mumps virus using 96-well tissue culture plate and PAP (peroxidase-anti-peroxidase) staining technique. Microbiol. Immunol.29, (1985). (In press.)
Quina, M. A., Thein, S., Auwanich, W., Okuno, Y., Igarashi, A., Fukai, K.: Changes in dengue and Japanese encephalitis (JE) antibody after JE vaccination. Biken J.21, 149–159 (1978).
Susilowati, S., Okuno, Y., Fukunaga, T., Tadano, M., Juang, R. F., Fukai, K.: Neutralization antibody responses induced by Japanese encephalitis vaccine. Biken J.24, 137–145 (1981).
Tadano, M., Okuno, Y., Fukunaga, T., Fukai, K.: Retrospective serological studies on dengue epidemics in Osaka and Okinawa. Biken J.26, 165–167 (1983).
Tanishita, O., Takahashi, Y., Okuno, Y., Yamanishi, K., Takahashi, M.: Evaluation of focus reduction neutralization test with peroxidase-antiperoxidase staining technique for hemorrhagic fever with renal syndrom virus. J. Clin. Microbiol.20, 1213–1215 (1984).
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Okuno, Y., Fukunaga, T., Tadano, M. et al. Rapid focus reduction neutralization test of Japanese encephalitis virus in microtiter system. Archives of Virology 86, 129–135 (1985). https://doi.org/10.1007/BF01314119
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DOI: https://doi.org/10.1007/BF01314119