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Interactions of Pseudorabies virus with swine alveolar macrophages I: virus replication

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Summary

The replication of Pseudorabies virus (PRV) in cultured swine alveolar macrophages (AM) was studied using 6 different virus strains. AM were highly permissive to PRV infection, with progeny virus titres of 107 TCID50/ml from some strains. Virus progeny titres were higher in cultures infected with the field strains S-62 and 4892 than in cultures infected with the strains Bartha or PRV-C. Virus replication, viral DNA synthesis and the concomitant cell damage were dependent upon virus input m.o.i.s. and virus strain. Furthermore, cells from 7 day old pigs yielded higher virus progeny titres than cells of 6 week old pigs. The results from this study provide support to the premise that PRV infection may predispose anials to respiratory disease.

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  1. G. Iglesias

    Present address: Department of Food Animal and Euqine Medicine, School of Veterinary Medicine, Raleigh, NC, USA

Authors and Affiliations

  1. Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota, USA

    G. Iglesias, C. Pijoan & T. Molitor

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  1. G. Iglesias
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  2. C. Pijoan
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  3. T. Molitor
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Iglesias, G., Pijoan, C. & Molitor, T. Interactions of Pseudorabies virus with swine alveolar macrophages I: virus replication. Archives of Virology 104, 107–115 (1989). https://doi.org/10.1007/BF01313812

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  • DOI: https://doi.org/10.1007/BF01313812

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