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Destabilization of herpes simplex virus type 1 virions by local anesthetics, alkaline pH, and calcium depletion

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Summary

The infectivity of herpes simplex virus type 1 (HSV-1) was found to be markedly reduced by treating virions with the tertiary amine local anesthetics lidocaine, dibucaine and tetracaine. These treatments induced a characteristic shift in the buoyant density of the HSV-1 particles from a “light” to a “heavy” population. HSV-1 virions were unstable at alkaline pH, and alkali treatment caused the same shift in buoyant density. Ca2+ stabilized the “light” population. These results suggest that the physicochemical status of the HSV-1 envelope which is sensitive to the treatments described above plays an important role for the integrity of the virion.

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Authors and Affiliations

  1. Department of Virology and Rickettsiology, National Institute of Health, Shinagawaku, Tokyo, Japan

    Kazuo Yanagi & Shizuko Harada

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  1. Kazuo Yanagi
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  2. Shizuko Harada
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Yanagi, K., Harada, S. Destabilization of herpes simplex virus type 1 virions by local anesthetics, alkaline pH, and calcium depletion. Archives of Virology 108, 151–159 (1989). https://doi.org/10.1007/BF01313753

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  • DOI: https://doi.org/10.1007/BF01313753

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