Summary
Measles virus grown in Vero cell cultures was examined at the ultra-structural level after immunoperoxidase staining with antiserum against the matrix protein. The antiserum clearly preferentially labeled the fuzzy material surrounding cytoplasmic nucleocapsids, but not the nucleocapsids themselves.
References
Brown HR, Thormar H (1976) A non-productive subacute sclerosing panence-phalitis (SSPE) virus of human and ferret. Acta Neuropathol 34: 339–351
Brown HR, Thormar H, Lin FH (1980) Immunolabeling of SSPE and wild-type measles viruses in ferret brain cell culture. Acta Neuropathol 50: 181–186
Dubois-Dalcq M, Barbosa LH (1973) Immunoperoxidase stain of measles antigen in tissue culture. J Virol 12: 909–918
Dubois-Dalcq M, Horta-Barbosa L, Hamilton R, Sever JL (1974) Comparison between productive and latent SSPE viral infectionin vitro. Lab Invest 30: 241–250
Johnson KP, Norrby E, Swoveland P, Carrigan DR (1981) Experimental subacute sclerosing panencephalitis: selective disappearance of measles virus matrix protein from the central nervous system. J Infect Dis 144: 161–169
McLean IW, Nakane PK (1974) Periodate-lysine-paraformaldehyde. A new fixative for immunoelectron microscopy. J Histochem Cytochem 22: 1077–1083
McSharry JJ, Compans RW, Lackland H, Choppin PW (1975) Isolation and characterization of nonglycosylated membrane protein and a nucleocapsid complex from the paramyxovirus SV 5. Virology 67: 365–374
Stallcup KC, Wechsler SL, Fields BN (1979) Purification of measles virus and characterization of subviral components. J Virol 30: 166–176
Tyrrell DLJ, Rafter DJ, Orvell C, Norrby E (1980) Isolation and immuno-logical characterization of the nucleocapsid and membrane proteins of measles virus. J Gen Virol 51: 307–315
Varsanyi TM, Utter G, Norrby E (1984) Purification, morphology and antigenic characterization of measles virus envelope components. J Gen Virol 65: 355–366
Yoshida T, Nagai Y, Yoshii S, Maeno K, Matsumoto T, Hoshino M (1976) Membrane (M) protein of HVJ: its role in virus assembly. Virology 71: 141–161
Author information
Authors and Affiliations
Additional information
With 4 Figures
Rights and permissions
About this article
Cite this article
Brown, H.R., Goller, N., Thormar, H. et al. Fuzzy material surrounding measles virus nucleocapsids identified as matrix protein. Archives of Virology 94, 163–168 (1987). https://doi.org/10.1007/BF01313735
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01313735