Summary
Marin County virus (MCV) was isolated from a stool suspension and serially propagated in human embryonic kidney cell cultures. MCV particles in stool and cell-propagated virus stocks showed reactivity by immune electron microscopy (IEM) with rabbit antiserum to astrovirus type 5. MCV antigen was also detected in two MCV stool samples by enzyme immunoassay (EIA) with an astrovirus group-specific monoclonal antibody. Acute and convalescent sera from 3 of 3 MCV-infected patients showed seroconversion to cell-propagated MCV by EIA. Immunofluorescence of MCV propagated in cell culture showed positive reactivity with an astrovirus group specific monoclonal antibody and astrovirus type 5 antiserum, with some cross-reactivity with astrovirus type 1. Similar results were obtained with the prototype strain of astrovirus type 5. However, in plaque-reduction assays, both the prototype astrovirus type 5 and MCV were neutralized by type 5 antiserum only. We conclude that MCV can be serially propagated by techniques used for previously described astroviruses and is serotypically an astrovirus type 5.
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Cubitt WD, McSwiggan DA, Moore W (1979) Winter vomiting disease caused by a calicivirus. Clin Pathol 32: 786–793
Herrmann JE, Hendry RM, Collins MF (1979) Factors involved in enzyme-linked immunoassay and evaluation of the method for identification of enteroviruses. J Clin Microbiol 10: 210–217
Herrmann JE, Hudson RW, Blacklow NR, Cubitt WD (1987) Marin County agent, an astrovirus. Lancet 2: 743
Herrmann JE, Hudson RW, Perron-Henry DM, Kurtz JB, Blacklow NR (1988) Antigenic characterization of cell-cultivated astrovirus serotypes and development of astrovirus-specific monoclonal antibodies. J Infect Dis 158: 182–185
Hudson RW, Herrmann JE, Blacklow NR (1989) Plaque quantitation and virus neutralization assays for human astroviruses. Arch Virol 108: 33–38
Kurtz JB, Lee TW (1984) Human astrovirus serotypes. Lancet 2: 1405
Lee TW, Kurtz JB (1981) Serial propagation of astroviruses in tissue culture with the aid of trypsin. J Gen Virol 57: 421–424
Oshiro LS, Haley CE, Roberto RR, Riggs JL, Croughan M, Greenberg H, Kapikian A (1981) A 27-nm virus isolated during an outbreak of acute infectious nonbacterial gastroenteritis in a convalescent hospital: a possible new serotype. J Infect Dis 143: 791–795
Wilson SA, Cubitt WD (1988) The development and evaluation of radioimmune assays for the detection of immune globulins M and G against astrovirus. J Virol Methods 19: 151–160
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Herrmann, J.E., Cubitt, W.D., Hudson, R.W. et al. Immunological characterization of the Marin County strain of astrovirus. Archives of Virology 110, 213–220 (1990). https://doi.org/10.1007/BF01311289
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DOI: https://doi.org/10.1007/BF01311289