Summary
Rabies virus strains isolated from a European bat (Eptesicus serotinus) in Denmark (DBV), a North American big brown bat (Eptesicus fuscus) in New York State (NY-bat), and a human in South Africa (Duvenhage strain (DUV-1) were studied by using a panel of monoclonal antibodies and by inoculating mice, cats, and dogs. The ten Danish virus isolates from the same bat species reacted identically with a panel of monoclonal antibodies. Immunofluorescence, monoclonal antibody, and histopathologic studies showed that the Danish bat isolates were similar to Duvenhage, and to some degree, to classical rabies virus. All isolates produced fatal infections in mice when inoculated by the intracerebral, footpad, and oral routes. Dogs and cats inoculated intracerebrally with the DBV and DUV-1 virus strains died of rabies-like illnesses within 10 days. Although no dogs that were inoculated intramuscularly or intravenously showed signs of disease, all developed neutralizing antibodies and resisted challenge with lethal dose of street rabies virus. All dogs inoculated with the NY-bat virus, with the exception of those inoculated intravenously, showed classical signs of rabies and one of the intramuscularly inoculated dogs recovered. Cats inoculated intramuscularly also died of rabies-like illness within 15 days. At necropsy, rabies antigen was detected by immunofluorescence in frozen sections of several organs, including brain and salivary glands. Histopathologic and electron microscopic studies of the central nervous system of mice, dogs and cats that died of DBV infection showed neuronal cytoplasmic changes considered to be a form of spongiosis.
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References
Andrews JM, Gardner MB (1974) Lower motor neuron degeneration associated with type C RNA virus infection in mice: neuropathological features. J Neuropathol Exp Neurol 33: 285–307
Baer GM (1975) Bovine paralytic rabies and rabies in the vampire bat. In: Baer GM (ed) Natural history of rabies, vol 2. Academic Press, New York, pp 155–175
Baer GM (1975) Rabies in non-hemotophagous bats. In: Baer GM (ed) Natural history of rabies, vol 2. Academic Press, New York, pp 79–89
Beck E, Daniel PM, Asher DM, Gajdusek DC, Gibbs CJ Jr (1973) Experimental kuru in the chimpanzee. A neuropathological study. Brain 96: 441–462
Beck E, Daniel PM (1979) Kuru and Creutzfeldt-Jakob disease: neuropathological lesions and their significance. In: Pruisner SB, Hadlow WT (eds) Slow transmissible diseases of the neurous system, vol 1. Academic Press, New York, pp 253–270
Beck E, Daniel PM, Davey AJ, Gajdusek DC, Gibbs CJ Jr (1982) The pathogenesis of transmissible spongiform encephalopathy: an ultrastructural study. Brain 105: 755–786
Boulger LR, Porterfield JS (1958) Isolation of a virus from Nigeria fruit bats. Trans R Soc Trop Med 52: 421–424
Charlton KM (1984) Rabies: spongiform lesions in the brain. Acta Neuropathol (Berl) 63: 198–202
Constantine DG (1962) Rabies transmission by nonbite route. Public Health Rep 77: 287–289
Constantine DG, Woodall DF (1966) Transmission experiments with bat rabies isolates: reactions of certain carnivora, opossum, rodents and bats to rabies virus of red bat origin when exposed by bat bite or intramuscular inoculation. Am J Vet Res 27: 24–32
Constantine DG (1966) Transmission of rabies to foxes and coyotes by freetail bats. Am J Vet Res 27: 20–23
Familusi JB, Moore DL (1972) Isolation of a rabies-related virus from the cerebrospinal fluid of a child with aseptic meningitis. Afr J Med Sci 3: 93–96
Familusi JB, Osunkoya BO, Moore DL, Kemp GE, Fabiyi A (1972) A fatal human infection with Mokola virus. Am J Trop Med Hyg 21: 959–963
Fischman HR, Ward FE (1968) Oral transmission of rabies virus in experimental animals. Am J Epidemiol 88: 132–138
Fekadu M, Chandler FW, Harrison AK (1982) Pathogenesis of rabies in dogs inoculated with an Ethiopian rabies virus strain. Immunofluorscence, histopathologic, and ultrastructural studies of the central nervous system. Arch Virol 71: 109–126
Fekadu M, Greer PW, Chandler FW, Sanderlin DW (1988) Use of the avidin-biotin peroxidase system to detect rabies antigen in formalin-fixed paraffin-embedded tissues. J Virol Methods 19: (in print)
Foggin CM (1980) Atypical rabies virus in cats and a dog in Zimbabwe. Vet Rec 110: 338
Foggin CM (1983) Mokola virus infection in cats and a dog in Zimbabwe. Vet Rec 113: 115
Foggin CM, Swanepoel R (1985) Rabies in Africa with emphasis on rabies-related viruses. In: Koprowski H, Plotkin SA (eds) World's debt to Pasteur. Alan R Liss, Philadelphia, pp 219–234
Goldwasser RA, Kissling RE (1958) Fluorescent antibody staining of street and fixed rabies virus antigen. Proc Soc Exp Biol Med 98: 219–223
Johnson RT (1983) Viral infections of the nervous system. Raven Press, New York, pp 271–293
Kemp GE, Moore DL, Odelola A, Fabiyi A (1972) Further studies on the 27377, a new rabies-related etiologic agent of zoonosis in Nigeria. Am J Trop Med Hyg 21: 356–359
Kemp GE, Lee VH, Moore DL, Shope RE, Causey OR, Murphy RA (1973) Kontonkan, a new rhabdovirus related to Mokola virus of the rabies group. Am J Epidemiol 98: 43–49
Kemp GE, Moore DL, Isoun TT, Fabiyi A (1973) Mokola virus: experimental infection and transmission studies with the shrew, a natural host. Arch Ges Virusforsch 43: 242–250
Kemp GE, Causey OR, Setzer HW, Moore DL (1974) Isolation of viruses from wild mammals in West Africa, 1966–1970. J Wildl Dis 10: 279–293
Kliiver H, Barrera EA (1953) Methods for the combined staining of cells and fibers in the nervous system. J Neuropathol Exp Neurol 12: 400–403
Lampert PW, Gajdusek DC, Gibbs CJ Jr (1972) Subacute spongiform virus encephalopathies. Scrapie, Kuru, and Creutzfeldt-Jakob diseases: a review. Am J Pathol 68: 626–652
Meredith CD, Rossouw AP, Van Praag Koch H (1971) An unusual case of human rabies thought to be of chiropteran origin. S Afr Med J 45: 767–769
Mollenhauer HH (1964) Plastic embedding mixtures for use in electron microscopy. Stain Technol 39: 111
Mollgaard S (1985) Bat rabies in Denmark. Rabies Bulletin Europe, Rabies Surveillance Report 3/85, 8–11
Murphy FA, Bauer SP, Harrison AK, Winn WC (1973) Comparative pathogenesis of rabies and rabies-like viruses. Viral infection and transit from inoculation site to the central nervous system. Lab Invest 28: 361–376
Murphy FA, Harrison AK, Winn WC, Bauer SP (1973) Comparative pathogenesis of rabies and rabies-like viruses. Infection of the central nervous system and centrifugal spread to peripheral tissues. Lab Invest 29: 1–16
Murphy FA, Bell JF (1977) Pathogenesis, brief review. Arch Virol 54: 279–297
Percy DH, Bhatt PN, Tignor GH, Shope RE (1973) Experimental infection of dogs and monkeys with two rabies serogroup viruses, Lagos bat and Mokola (IbAn 27377). Gross pathologic and histopathologic changes. Vet Pathol 10: 534–549
Rabies Bulletin Europe 4: 17–18, 1985
Schneider LG, Bernard BJH, Schneider HP (1983) Application of monoclonal antibodies for epidemiologic investigations and oral vaccine studies. I. African viruses. Proc Int Conf Rabies in the Tropics, Tunis, 3–6 October, 1983
Shope RE, Murphy FA, Harrison AK, Causey OR, Kemp RE, Simpson DL, Moore DL (1970) Two African viruses serologically and morphologically related to rabies virus. J Virol 6: 690–692
Selimov MA, Tatarov AG, Antonova LA, Shcherbak YN, Shablovskaya EA, Smekhov AM, Mogilevsky BY (1986) To the issue of chiropteric rabic infection. Rabies Information Exchange 14 (June 1986): 9–12
Smith JS, Sumner JW, Roumillat LF, Baer GM, Winkler WG (1984) Antigenic characteristics of isolates associated with a new epizootic of raccoon rabies in the United States. J Infect Dis 149: 769–774
Smith JS, Yager PA, Baer GM (1973) A rapid reproducible test for determining rabies neutralizing antibody. Bull WHO 48: 535–541
Tignor GH, Murphy FA, Clark HF Shope RE, Madore P, Bauer SP, Buckley SM, Meredith CD (1977) Duvenhage virus: morphological, biochemical, histopathological, and antigenic relationships to the rabies serogroup. J Gen Virol 37: 595–611
Tignor GH, Shope RE, Bhatt PN, Percy DH (1973) Experimental infection of dogs and monkeys with two rabies serogroup viruses, Lagos bat and Mokola (IbAn 27377): clinical, serologic, virologic, and fluorescent antibody studies. J Infect Dis 128: 471–478
Trimarchi CV, Rudd RJ, Abelseth MK (1986) Experimentally induced rabies in four cats inoculated with a rabies virus isolated from a bat. Am J Vet Res 47: 777–780
Van der Merwe M (1982) Bats as vectors of rabies. S Afr J Sci 78: 421–422
Wiktor TJ, Flamand A, Koprowski H (1980) Use of monoclonal antibodies in diagnosis of rabies virus infection and differentiation of rabies and rabies related viruses. J Virol Methods 1: 33–46
World Health Organization (WHO) (1982) World survey of rabies 21: 1982/83. WHO, Geneva, Switzerland
Winkler WG (1968) Airborne rabies virus isolation. Bull Wildl Dis 4: 37–40
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Fekadu, M., Shaddock, J.H., Chandler, F.W. et al. Pathogenesis of rabies virus from a Danish bat (Eptesicus serotinus): neuronal changes suggestive of spongiosis. Archives of Virology 99, 187–203 (1988). https://doi.org/10.1007/BF01311069
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DOI: https://doi.org/10.1007/BF01311069