Summary
Genomic sequences of pseudorabies virus, a porcine herpesvirus, were amplified by the polymerase chain reaction from cells of infected cultures, nasal cells and organs from acutely diseased as well as from organs of latently infected pigs.
References
Belák S, Rockborn G, Wierup M, Belák K, Berg M, Linné T (1987) Aujeszky's disease diagnosed by a simple method of nucleic acid hybridization. J Vet Med B 34: 519–529
Belák S, Linné T (1988) Rapid detection of Aujeszky's disease (pseudorabies) virus infection in pigs by direct filter hybridization of nasal and tonsillar specimens. Res Vet Sci 44: 303–308
Belák K, Funa K, Kelly R, Belák S (1989) Rapid diagnosis of Aujeszky's disease by improved in situ hybridization using biotinylated probes on paraffin-embedded tissue sections. J Vet Med B 36: 10–20
Ben-Porat T, Deatly AM, Easterday DC, Galloway D, Kaplan AS, McGregor S (1984) Latency of pseudorabies virus. Curr Top Vet Med Anim Sci 27: 365–383
Davis LG, Dibner MD, Battey JF (1986) Basic methods in molecular biology. Elsevier, New York
Guitteny AF, Fouque B, Mougin C, Teoule R, Bloch B (1988) Histological detection of messenger RNAs with biotinylated synthetic oligonucleotide probes. J Histochem Cytochem 36: 563–571
Gutekunst DE (1979) Latent pseudorabies virus infection in swine detected by RNA-DNA hybridization. Am J Vet Res 40: 1568–1572
Linné T (1987) Diagnosis of pseudorabies infection in swine with specific DNA probes. Res Vet Sci 43: 150–156
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Misra V, Nelson R, Smith M (1988) Sequence of bovine herpesvirus type-1 glycoprotein gene that is homologous to the herpes simplex gene for the glycoprotein gB. Virology 166: 542–549
McFarlane RG, Thawley DG, Solorzano RF (1986) Detection of latent pseudorabies virus in porcine tissue, using a DNA hybridization dot-blot assay. Am J Vet Res 47: 2329–2336
McFerran JB, McCracken RM, Dow C (1984) The role of the carrier pig in the epidemiology of Aujeszky's disease. Curr Top Vet Med Anim Sci 27: 403–415
Mullis KB, Faloona FA (1987) Specific synthesis of DNA in vitro via a polymerase catalysed chain reaction. Methods Enzymol 155: 335–350
Robbins AK, Dorney DJ, Wathen MW, Whealy ME, Gold C, Watson RJ, Holland LE, Weed SD, Levine M, Glorioso JC, Enquist LW (1987) The pseudorabies virus gII gene is closely related to the gB glycoprotein gene of herpes simplex virus. J Virol 61: 2691–2701
Rock DL, Hagemoser WA, Osorio FA, McAllister HA (1988) Transcription from the pseudorabies virus genome during latent infection. Arch Virol 98: 99–106
Rziha H-J, Mettenleiter TC, Ohlinger V, Wittmann G (1986) Herpesvirus (pseudorabies virus) latency in swine: occurrence and physical state of viral DNA in neural tissue. Virology 155: 600–613
Saiki RK, Scharf S, Faloona FA, Mullis KB, Horn GT, Ehrlich HA, Arnheim N (1985) Enzymatic amplification of β-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 230: 1350–1354
Salimans MMM, Holsappel S, van de Rijke FM, Jiwa NM, Raap AK, Weiland HT (1989) Rapid detection of human parvovirus B19 DNA by dot-hybridization and the polymerase chain reaction. J Virol Methods 23: 19–28
Shibata DK, Arnheim N, Martin WJ (1988) Detection of human papilloma virus in paraffin-embedded tissue using the polymerase chain reaction. J Exp Med 167: 225–230
Whitbeck JC, Bello LJ, Lawrence WC (1988) Comparison of the bovine herpesvirus 1 gI gene and the herpes simplex virus type 1 gB gene. J Virol 62: 3319–3327
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On leave from: Department of Epizootiology, University of Veterinary Sciences, Budapest, Hungary.
On leave from: Department of Agricultural Chemical Technology, Technical University, Budapest, Hungary.
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Belák, S., Ballagi-Pordány, A., Flensburg, J. et al. Detection of pseudorabies virus DNA sequences by the polymerase chain reaction. Archives of Virology 108, 279–286 (1989). https://doi.org/10.1007/BF01310940
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DOI: https://doi.org/10.1007/BF01310940