Summary
The two biologically distinct strains of murine cytomegalovirus, “Smith” and “K181”, showed slightly different restriction-endonuclease profiles; but we could not detect strain differences in the numbers or sizes of the major immediate-early, early or late viral proteins. It is possible however that some of the minor late proteins may differ.
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References
Centifanto-Fitzgerald YM, Yamaguchi T, Kaufmann HE, Togon M, Roizman B (1982) Ocular disease pattern induced by herpes simplex virus is genetically determined by a specific region of viral DNA. J Exp Med 155: 475–489
Chantler JK, Hudson JB (1978) Proteins of murine cytomegalovirus: identification of structural and non-structural antigenes in infected cells. Virology 86: 22–36
Ebeling A, Keil GM, Knust E, Koszinowski UH (1983) Molecular cloning and physical mapping of murine cytomegalovirus DNA. J Virol 47: 421–433
Hudson JB (1979) The murine cytomegalovirus as a model for the study of viral pathogenesis and persistent infections. Arch Virol 62: 1–29
Hudson JB (1984) Factors involved in persistent murine cytomegalovirus infections—A review. In: Wittman G, Gaskell RM, Rziha J-J (eds) Latent herpesvirus infections in veterinary medicine. Martinus Nijhoff, The Hague, pp 127–147
Keil GM, Ebeling-Keil A, Koszinowski UH (1984) Temporal regulation of murine cytomegalovirus transcription and mapping of viral RNA synthesized at immediate-early times after infection. J Virol 50: 784–795
Keil GM, Ebeling-Keil A, Koszinowski UH (1987) Immediate-early genes of murine cytomegalovirus: location, transcripts and translation products. J Virol 61: 526–533
Lomniczi B, Watanabe S, Ben-Porat T, Kaplan AS (1984) Genetic basis of the neurovirulence of pseudorabies virus. J Virol 52: 198–205
Lomniczi B, Gielkens A, Csobai I, Ben-Porat T (1987) Evolution of pseudorabies virions containing genomes with an invertible long component after repeated passage in chicken embryo fibroblasts. J Virol 1772–1780
Marks JR, Mercer JR, Spector DH (1983) Transcription in mouse embryo cells permissively infected by murine cytomegalovirus. Virology 131: 247–254
Mercer JA, Marks JR, Spector DH (1983) Molecular cloning and restriction endonuclease mapping of the murine cytomegalovirus genome (Smith strain). Virology 129: 94–106
Misra V, Hudson JB (1980) Minor base sequence differences between the genomes of two strains of murine cytomegalovirus differing in virulence. Arch Virol 64: 1–8
Moon HM, Sapienza VJ, Carp RI, Kim KS (1979) Murine cytomegalovirus-induced protein synthesis. J Gen Virol 42: 159–169
Mosmann TR, Hudson JB (1974) Structural and functional heterogeneity of the murine cytomegalovirus genome. Virology 62: 175–183
Osborn JE (1982) CMV-herpesviruses of mice. In: Foster HL, Fox JG, Small JD (eds) The mouse in biomedical research, vol. 2. Academic Press, New York, pp 267–292
Rosen A, Ernst F, Koch H-G, Gelderblom H, Darai G, Hadar J, Tabor E, Ben-Hur T, Becker Y (1986) Replacement of the deletion in the genome (0.762–0.789 mu) of avirulent HSV-1 HFEM using cloned M1uI DNA fragment (0.7615–0.796 mu) of virulent HSV-1 F leads to generation of virulent intratypic recombinant. Virus Res 5: 157–175
Thompson RL, Devi-Rao GV, Stevens JG, Wagner EK (1985) Rescue of a herpes simplex virus type 1 neurovirulence function with a cloned DNA fragment. J Virol 55: 504–508
Walker D, Hudson JB (1987) Analysis of immediate-early and early proteins of murine cytomegalovirus in permissive and nonpermissive cells. Arch Virol 92: 103–119
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Hudson, J.B., Walker, D.G. & Altamirano, M. Analysis in vitro of two biologically distinct strains of murine cytomegalovirus. Archives of Virology 102, 289–295 (1988). https://doi.org/10.1007/BF01310834
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DOI: https://doi.org/10.1007/BF01310834