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Serine 71 of the glycoprotein HEF is located at the active site of the acetylesterase of influenza C virus

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Summary

The acetylesterase of influenza C virus has been reported recently to be inhibited by diisopropylfluorophosphate (DFP) [Muchmore EA, Varki A (1987) Science 236: 1293–1295]. As this inhibitor is known to bind covalently to the serine in the active site of serine esterases, we attempted to determine the serine in the active site of the influenza C acetylesterase. Incubation of purified influenza C virus with3H-DFP resulted in the selective labelling of the influenza C glycoprotein HEF. The labelled glycoprotein was isolated from a SDS-polyacrylamide gel. Following reduction and carboxymethylation, tryptic peptides of HEF were prepared and analyzed by reversed phase HPLC. The peptide containing the3H-DFP was subjected to sequence analysis. The amino acids determined from the NH2-terminus were used to locate the peptide on the HEF polypeptide. Radiosequencing revealed that3H-DFP is attached to amino acid 17 of the tryptic peptide. These results indicate that serine 71 is the active-site serine of the acetylesterase of influenza C virus.

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Authors and Affiliations

  1. Institut für Virologie, Philipps-Universität Marburg, Marburg, Federal Republic of Germany

    G. Herrler & H. -D. Klenk

  2. Zentrum für Molekulare Biologie, Universität Heidelberg, Heidelberg, Federal Republic of Germany

    G. Multhaup & K. Beyreuther

Authors
  1. G. Herrler
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  2. G. Multhaup
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  3. K. Beyreuther
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  4. H. -D. Klenk
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Herrler, G., Multhaup, G., Beyreuther, K. et al. Serine 71 of the glycoprotein HEF is located at the active site of the acetylesterase of influenza C virus. Archives of Virology 102, 269–274 (1988). https://doi.org/10.1007/BF01310831

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  • DOI: https://doi.org/10.1007/BF01310831

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