Summary
The infection rate of an established colony ofCulicoides variipennis to oral infection with bluetongue virus (BTV) type 4 was found to be highly variable and ranged from 0 to 51.6 per cent. This type of wide variation has previously only been reported to occur between individual populations of a species and not between samples from a single population. This must be taken into account when such colonies are used as reference standards for interpretation of field and laboratory data, particularly relating to studies of vector efficiency.
Study of the response of individual susceptibleC. variipennis to BTV infection demonstrated considerable variation in the level of virus multiplication that individual females are able to support. Virus concentrations varied from less than 1.1 to 5.1 log10 TCID50/fly up to 10 days post infection. 43.6 per cent of females contained less than 2.5 log10 TCID50 virus. It is suggested that such insects would have a mesenteron escape barrier to infection and would be incapable of transmitting BTV. The implications of these results for vector research are discussed.
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References
Boorman J (1974) The maintenance of laboratory colonies ofCulicoides variipennis (Coq.)C. nubeculosus (Mg). andC. riethi (Kieff) (Diptera, Ceratopogonidae). Bull Entomol Res 64: 371–377
Finney DJ (1964) Statistical methods in biological assay. 2nd edn. Griffin, London, p 668
Foster NM, Jones RH (1973) Bluetongue virus transmission withCulicoides variipennis via embryonating chicken eggs. J Med Entomol 10: 529–532
Foster NM, Jones RH, Walton TE (1981)Culicoides variipennis infection and transmission rates for virulent bluetongue virus. Abstr Ann Meet Am Soc Microbiol 1981, p 250
Gargan TP, Bailey CL, Higbee GA, Gad A, El Said S (1983) The effect of laboratory colonisation on the vector pathogen interaction of EgyptianCulex pipiens and Rift valley fever virus. Am J Trop Med Hyg 32: 1154–1163
Jennings M, Boorman J (1980) Use of the indirect fluorescent antibody technique for the detection of bluetongue virus antigen in tissue smears fromCulicoides variipennis (Diptera, Ceratopogonidae). Vet Microbiol 5: 13–18
Jones RH, Foster NM (1971) Oral infection ofCulicoides variipennis with bluetongue virus: Development of susceptible and resistant lines from a colony population. J Med Entomol 11: 316–323
Jones RH, Foster NM (1978a) Relevance of laboratory colonies of the vector in arbovirus research—Culicoides variipennis and bluetongue. Am J Trop Med Hyg 27: 168–177
Jones RH, Foster NM (1978b) Heterogeneity ofCulicoides variipennis field populations to oral infection with bluetongue virus. Am J Trop Med Hyg 27: 178–183
Kramer LD, Hardy JL, Presser SB, Houk EJ (1981) Dissemination barrier for Western Equine Encephalomyelitis inCulex tarsalis infected after ingestion of low virus doses. Am J Trop Med Hyg 30: 1130–1139
Mellor PS, Boorman J, Loke R (1974) The multiplication of Maindrain virus in two species ofCulicoides (Diptera, Ceratopogonidae). Arch Ges Virusforsch 46: 105–110
Parker J, Herniman KAJ, Gibbs EPJ, Sellers RF (1975) An experimental inactivated vaccine against bluetongue. Vet Rec 96: 284–287
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Jennings, D.M., Mellor, P.S. Variation in the responses of Culicoides variipennis (Diptera, Ceratopogonidae) to oral infection with bluetongue virus. Archives of Virology 95, 177–182 (1987). https://doi.org/10.1007/BF01310778
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DOI: https://doi.org/10.1007/BF01310778