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Evidence for endocytosis-independent infection by human rotavirus

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Summary

The effects of five lysosomotropic drugs (NH4Cl, chloroquine, methylamine, amantadine and dansylcadaverine) and cytochalasin B on human rotavirus (HRV KUN strain) and vesicular stomatitis virus (VSV Indiana strain) infection in monkey MA 104 cells were examined. These drugs had little effect on HRV yield but greatly reduced VSV yield. The results strongly suggest that HRV does not require endocytotic activity and intracellular acidic vesicles for the initial stage of infection and support our postulate that HRV enters the target cell by direct penetration of its nucleoid through the cell membrane.

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Authors and Affiliations

  1. Department of Bacteriology, Tohoku University School of Medicine, Sendai, Japan

    N. Fukuhara & O. Yoshie

  2. Department of Pediatries, Research Institute for Tuberculosis and Cancer, Tohoku University, Sendai, Japan

    S. Kitaoka & T. Konno

  3. Tohoku University, Sendai, Japan

    N. Ishida

Authors
  1. N. Fukuhara
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  2. O. Yoshie
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  3. S. Kitaoka
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  4. T. Konno
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  5. N. Ishida
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Fukuhara, N., Yoshie, O., Kitaoka, S. et al. Evidence for endocytosis-independent infection by human rotavirus. Archives of Virology 97, 93–99 (1987). https://doi.org/10.1007/BF01310737

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  • DOI: https://doi.org/10.1007/BF01310737

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