Skip to main content
SpringerLink
Log in

Conformational specificity of monoclonal antibodies used in the diagnosis of tomato mosaic virus

  • Original Papers
  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

Ten monoclonal antibodies (McAbs) were raised to the tobamovirus, tomato mosaic virus (ToMV). Seven of the McAbs reacted with ToMV only when tested in an ELISA format, involving antibody-coated plates, which preserved the quaternary conformation of the viral protein. Two McAbs which reacted with ToMV only in an ELISA using virus-coated plates recognized an antigenic determinant present only on the disassembled form of the viral coat protein. One McAb reacted with both the assembled and dissociated forms of ToMV protein.

All McAbs reacted in an identical manner with the 15 ToMV strains and isolates tested, confirming the previously established serological homogeneity of the ToMV species. Only two of the McAbs cross-reacted with two other tobamoviruses: tobacco mosaic virus (TMV) and ribgrass mosaic virus. Seven variations of ELISA were compared for their suitability to detect ToMV and TMV in tomato plants. Double antibody sandwich ELISA could be performed with a single McAb to ToMV as enough accessible epitopes are present on the virus to bind simultaneously to the coating and biotin-labelled antibody.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Al Moudallal Z, Briand JP, Van Regenmortel MHV (1982) Monoclonal antibodies as probes of the antigenic structure of tobacco mosaic virus. EMBO J 1: 1005–1010

    Google Scholar 

  2. Altschuh D, Al Moudallal Z, Briand JP, Van Regenmortel MHV (1985) Immunochemical studies of tobacco mosaic virus. VI. Attempts to localize viral epitopes with monoclonal antibodies. Mol Immunol 22: 329–337

    Google Scholar 

  3. Broadbent L (1965) The epidemiology of ToMV. II. Seed transmission of TMV. Ann Appl Biol 56: 177–205

    Google Scholar 

  4. Broadbent L (1976) Epidemiology and control of tomato mosaic virus. Ann Rev Phytopath 14: 75–96

    Google Scholar 

  5. Brunt AA (1986) Tomato mosaic virus. In:Van Regenmortel MHV, Fraenkel-Conrat H (eds) The plant viruses, vol 2. Plenum Press, New York, pp 181–197

    Google Scholar 

  6. Burgyan J, Beczner L, Gaborjanyi R (1978) Relationship among some tobamoviruses I. A symptomatological and serological comparison. Acta Phytopath Hung 13: 75–85

    Google Scholar 

  7. Diaco R, Lister RM, Hill JH, Durand DP (1986) Demonstration of serological relationship among isolates of barley yellow dwarf virus by using polyclonal and monoclonal antibodies. J Gen Virol 67: 353–362

    Google Scholar 

  8. Dietzgen RG (1986) Characterization of antigenic structures on arabis mosaic virus with monoclonal antibodies. Arch Virol 91: 163–173

    Google Scholar 

  9. Fazekas de St. Groth S, Scheidegger D (1980) Production of monoclonal antibodies: strategy and tactics. J Immunol Methods 35: 1–21

    Google Scholar 

  10. Friguet B, Djavadi-Ohaniance L, Goldberg ME (1984) Some monoclonal antibodies raised with a native protein bind preferentially to the denatured antigen. Mol Immunol 21: 673–677

    Google Scholar 

  11. Halk EL, Hsu HT, Aebig T, Franke J (1984) Production of monoclonal antibodies against three ilarviruses and alfalfa mosaic virus and their use in serotyping. Phytopathology 74: 367–372

    Google Scholar 

  12. Hill EK, Hill JH, Durand DP (1984) Production of monoclonal antibodies to viruses in the potyvirus group: use in radioimmunoassay. J Gen Virol 65: 525–532

    Google Scholar 

  13. Hollings M, Huttinga H (1976) Tomato mosaic virus. AAB/CMI Description of Plant Viruses, p 156

  14. Jaegle M, Van Regenmortel MHV (1985) Use of ELISA for measuring the extent of serological cross-reactivity between plant viruses. J Virol Methods 46: 63–68

    Google Scholar 

  15. Jerne NK (1960) Immunological speculations. Ann Rev Microbiol 14: 341–358

    Google Scholar 

  16. McCullough KC, Crowther JR, Butcher RN (1985) Alteration in antibody reactivity with foot-and-mouth disease virus (FDMV) 146 S antigen before and after binding to a solid phase or complexing with specific antibody. J Immunol Methods 82: 91–100

    Google Scholar 

  17. Mierendorf RC Jr, Dimond RL (1983) Functional heterogeneity of monoclonal antibodies obtained using different screening assays. Anal Biochem 135: 221–229

    Google Scholar 

  18. Pelham J (1972) Strain-genotype interaction of tobacco mosaic virus in tomato. Ann Appl Biol 71: 219–228

    Google Scholar 

  19. Rast ATB (1975) Variability of tobacco mosaic virus in relation to control of tomato mosaic in glasshouse tomato crops by resistance breeding and cross protection. Agric Res Rep (Wageningen) 834, p. 76

    Google Scholar 

  20. Sander E, Dietzgen RG (1984) Monoclonal antibodies against plant viruses. Adv Virus Res 29: 131–168

    Google Scholar 

  21. Stocker JW, Foster HK, Miggiano V, Stahli C, Staiger G, Takacs B, Staehelin Th (1982) Generation of two new myeloma cell lines “PAI” and “PAI-0” for hybridoma production. Res Disclosure 217: 155–157

    Google Scholar 

  22. Torrance L, Pead MT (1986) The application of monoclonal antibodies to routine tests for two plant viruses. In:Jones RAC, Torrance L (eds) Association of Applied Biologists. Wellesbourne, England, pp 103–118

    Google Scholar 

  23. Vaidya HC, Dietzler DN, Ladenson JH (1985) Inadequacy of traditional ELISA for screening hybridoma supernatants for murine monoclonal antibodies. Hybridoma 4: 271–276

    Google Scholar 

  24. Van Regenmortel MHV (1982) Serology and immunochemistry of plant viruses. Academic Press, New York, p 302

    Google Scholar 

  25. Van Regenmortel MHV (1984) Monoclonal antibodies in plant virology. Microbiol Sci 1: 73–78

    Google Scholar 

  26. Van Regenmortel MHV (1985) Antigenic structure of plant viruses. In:Van Regenmortel MHV, Neurath AR (eds) Immunochemistry of viruses. The basis for serodiagnosis and vaccines. Elsevier, Amsterdam, pp 467–478

    Google Scholar 

  27. Van Regenmortel MHV (1986) The potential for using monoclonal antibodies in the detection of plant viruses. In:Jones RAC, Torrance L (eds) Association of Applied Biologists. Wellesborune, England, pp 89–101

    Google Scholar 

  28. Van Regenmortel MHV, Burckard J (1980) Detection of a wide spectrum of tobacco mosaic virus strains by indirect enzyme-linked immunosorbent assays (ELISA). Virology 106: 327–334

    Google Scholar 

  29. Wang AL, Knight CA (1967) Analysis of protein components of tomato strains of tobacco mosaic virus. Virology 31: 101–106

    Google Scholar 

  30. Zrein M, Burckard J, Van Regenmortel MHV (1986) Use of the biotin-avidin system for detecting a broad range of serologically related plant viruses by ELISA. J Virol Methods 13: 121–128

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratoire d'Immunochimie, Institut de Biologie Moléculaire et Cellulaire, Strasbourg, France

    E. L. Dekker, I. Dore, C. Porta & M. H. V. van Regenmortel

Authors
  1. E. L. Dekker
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. I. Dore
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C. Porta
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. H. V. van Regenmortel
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

With 3 Figures

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dekker, E.L., Dore, I., Porta, C. et al. Conformational specificity of monoclonal antibodies used in the diagnosis of tomato mosaic virus. Archives of Virology 94, 191–203 (1987). https://doi.org/10.1007/BF01310713

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01310713

Keywords

Search

Navigation